Aliases &
Descriptions
for AKT1S1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| AKT1 substrate 1 (proline-rich)1 2 | | OTTHUMP000001967602 | | PRAS401 2 3 | | OTTHUMP000001967562 | | MGC28651 2 | | proline-rich AKT1 substrate 12 | | Lobe1 2 | | proline-rich Akt substrate, 40 kDa2 | | 40 kDa proline-rich AKT substrate2 3 | | |
Export aliases for AKT1S1 gene to outside databasesPrevious GC identifers: GC19M055067 GC19M055071 GC19M055073 GC19M050372 |
Summaries
for AKT1S1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for AKT1S1:
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) whenphosphorylated (Kovacina et al., 2003 (PubMed 12524439)).(supplied by OMIM)
UniProtKB/Swiss-Prot: AKTS1_HUMAN, Q96B36Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals.mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by RagGTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growthfactor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to theactivation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 andtargeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependenton its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substratefor AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nervegrowth factor-mediated neuroprotection
Gene Wiki entry for AKT1S1 |
Genomic Views
for AKT1S1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the AKT1S1 gene promoter:
p300 Sp1 NF-kappaB1 NF-kappaB NF-kappaB2 p53 FOXO3a FOXO3b GATA-1 Elk-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for AKT1S1
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for AKT1S1  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19q13.33 Ensembl cytogenetic band: 19q13.33 HGNC cytogenetic band: 19q13.33AKT1S1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19M046748: view genomic region
(about GC identifiers)
Start:
|
46,748,633 bp from pter |
End:
|
46,756,980 bp from pter |
Size:
|
8,348 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr19- 50,372,295-50,381,716 |
RefSeq DNA sequence:- NC_000019.9 NT_011109.16
|
Proteins
for AKT1S1 gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: AKTS1_HUMAN, Q96B36 (See
protein sequence)Recommended Name: Proline-rich AKT1 substrate 1 Size: 256 amino acids; 27383 Da
Subunit: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains FRAP1, LST8, RAPTOR and AKT1S1.mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interactswith 14-3-3 proteins
Subcellular location: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (Bysimilarity)
Sequence caution: Sequence=AAH00031.2; Type=Erroneous initiation;
Secondary accessions: A8MTQ1 B2RE93 Q96BI4 Q96IK7 Q96NG2 Q9BWR5Alternative splicing: 2 isoforms: Q96B36-1 Q96B36-2 Explore the universe of human proteins at neXtProt for AKT1S1: NX_Q96B36
Post-translational modifications:
Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC11
View phosphorylation sites using PhosphoSite2
REFSEQ proteins (3 alternative transcripts):
NP_001092102.1 NP_001092103.1 NP_115751.2
ENSEMBL proteins: ENSP00000375709 ENSP00000341698 ENSP00000375708 ENSP00000375711 ENSP00000375707 ENSP00000375706 ENSP00000375710
Human Recombinant Proteins
4 Gene Ontology (GO) cellular component terms (GO ID links to tree view): About this table
AKT1S1 for ontologies About GeneDecksing
Antibodies for AKT1S1:
Assays for AKT1S1: |
Protein
Domains/
Families
for AKT1S1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| ProtoNet protein and cluster: Q96B36 |
Gene Function
for AKT1S1 gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: AKTS1_HUMAN, Q96B36Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals.mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by RagGTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growthfactor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to theactivation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 andtargeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependenton its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substratefor AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nervegrowth factor-mediated neuroprotection
1 Gene Ontology (GO) molecular function term (GO ID links to tree view): About this table
AKT1S1 for ontologies About GeneDecksing
|
Pathways & Interactions
for AKT1S1 gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
AKT1S1 for pathways About GeneDecksing
1  Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathway for AKT1S1
1 
Cell Signaling Technology (CST) Pathway for AKT1S1
SABiosciences Pathway-Focused PCR Array including AKT1S1: PAHS-098A
Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for AKT1S1
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for AKT1S1
5/12 Interacting proteins for AKT1S1 (Q96B362 ENSP000003416983) via UniProtKB, MINT, and/or STRING (see all 12)About this table
5/8 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 8): About this table
AKT1S1 for ontologies About GeneDecksing
|
Drugs & Compounds
for AKT1S1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for AKT1S1
|
Transcripts
for AKT1S1 gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for AKT1S1 gene (3 alternative transcripts): NM_001098632.1 NM_001098633.1 NM_032375.3 Additional cDNA sequence: AK055511.1 AK092610.1 AK123687.1 AK226004.1 AK316603.1 BC000031.2 BC007416.2 BC015562.1 BC016043.1 BC022241.1 BC051844.2 CR595556.1 CR602166.1 CR603738.1 CR604133.1 CR606632.1 CR608170.1 CR608835.1 CR612202.1 CR619923.1 18 DOTS entries: DT.100701463 DT.95136694 DT.100878766 DT.91756880 DT.97765189 DT.91914533 DT.95245335 DT.97780687 DT.100878754 DT.95081511 DT.40225168 DT.121429460 DT.75107616 DT.95136686 DT.121429315 DT.121429347 DT.121429357 DT.100878747 24/756 AceView cDNA sequences (see all 756): AI139287 AA435912 AI307671 AX747627 BE790884 AI869032 AY358933 BU184116 BQ879836 NM_012346 BU161047 CD370347 AA731646 BE890106 BU542272 BI259746 BF436037 AI215639 BE256940 AA487508 AU132207 BU556816 BQ898301 AI864949
 highest scoring ESTs for AKT1S1:AI762096 AA577237 AI468603 AI824210 AV734459 BC015562 BC016043 BE267732 BE274297 BE276006 Unigene Cluster for AKT1S1: AKT1 substrate 1 (proline-rich) Hs.515542 [show with all ESTs]Unigene Representative Sequence: BC022241
GeneLoc Exon Structure
8 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000391833 ENST00000344175 ENST00000391832 ENST00000391835(uc002pqn.3 uc002pqm.3)
ENST00000391831 ENST00000391830 ENST00000482622 ENST00000391834(uc002pql.3)
|
Expression
for AKT1S1 gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| AKT1S1 expression in normal and diseased human tissues
1 / 2 / 3 6 probe-sets matching AKT1S1 gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 48732_at2, 3
|
U95-C |
1 |
1.00 |
1.00 |
0.62 |
1.20 |
W73033 |
0.60 |
1.00 |
0.82 |
1 |
| 89138_at2, 3
|
U95-E |
1 |
1.00 |
1.00 |
0.42 |
1.00 |
AI762096 |
1.00 |
0.87 |
0.94 |
1 |
| 66113_r_at2, 3
|
U95-C |
1 |
0.44 |
1.00 |
0.84 |
0.82 |
AI824210 |
0.60 |
1.00 |
0.82 |
1 |
| 224982_at2, 3
|
U133-B |
1 |
1.00 |
1.00 |
-- |
-- |
BE790884 |
0.60 |
0.78 |
0.69 |
1 |
| 1555821_a_at2
|
U133Plus2 |
1 |
1.00 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 224982_at2
|
U133Plus2 |
1 |
1.00 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
AKT1S1 for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: CGCGCGCTGG
SOURCE GeneReport for Unigene cluster: Hs.515542
UniProtKB/Swiss-Prot: AKTS1_HUMAN, Q96B36Tissue specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levelsin cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293)
SABiosciences Expression via Pathway-Focused PCR Array including AKT1S1: PAHS-098A
|
Orthologs
for AKT1S1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for AKT1S1 gene from 5/7 species (see all 7)
About this table Species with no ortholog for AKT1S1
ENSEMBL Gene Tree for AKT1S1 (if available) |
Paralogs
for AKT1S1 gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| -- |
Genomic Variants
for AKT1S1 gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 19 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for AKT1S1 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for AKT1S1
2 CNVs: 5114 32261 | | QIAGEN SeqTarget long-range PCR primers for resequencing AKT1S1 |
|
Disorders & Mutations
for AKT1S1 gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
AKT1S1 for disorders About GeneDecksing
OMIM: 610221
Export disorders and mutations for AKT1S1 gene to outside databases
|
Medical News
for AKT1S1 gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for AKT1S1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/24 PubMed articles for AKT1S1 gene, integrated from 7 sources (see all 24):
(articles sorted by number of sources associating them with AKT1S1)- Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. (PubMed id 12524439)1, 2, 3 Kovacina K.S.... Roth R.A. (2003)
- PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. (PubMed id 17386266)1, 2 Sancak Y....Sabatini D.M. (2007)
- Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. (PubMed id 17277771)1, 2 Vander Haar E....Kim D.H. (2007)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
- Expression of proline-rich Akt-substrate PRAS40 in cell survival pathway and carcinogenesis. (PubMed id 16174443)1, 2 Huang B. and Porter G. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. (PubMed id 19413330)2 Gauci S....Mohammed S. (2009)
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (2009)
- The binding of PRAS40 to 14-3-3 proteins is not required for activation of mTORC1 signalling by phorbol esters/ERK. (PubMed id 18215133)1 Fonseca B.D....Proud C.G. (2008)
|
External Searches for AKT1S1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing AKT1S1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing AKT1S1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing AKT1S1 gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for AKT1S1 gene:
Search GeneIP for patents involving AKT1S1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for AKT1S1 gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
|
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| | | |
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 |  |  |  | |
 | Antibodies & Assays for AKT1S1 (PRAS40) |
| | | | Search Tocris compounds for AKT1S1 |
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