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CRYGN Gene

protein-coding GIFtS: 41

GC07M144938

crystallin, gamma N
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases & Descriptions
crystallin, gamma N¹ ²		gammaN-crystallin²
gamma-N-crystallin²		MGC119043²
MGC119045²		gamma-crystallin N²
MGC119044²		Gamma-N-crystallin³
MGC119042²

External Ids:	HGNC: 20458¹	Entrez Gene: 155051²	Ensembl: ENSG00000127377⁷	UniProtKB: Q8WXF5³

Export aliases for CRYGN gene to outside databases

Previous GC identifers: GC07M149373 GC07M150440 GC07M150517 GC07M150564 GC07M150757 GC07M151125

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CRYGN:

The beta and gamma crystallins are evolutionarily related families of proteins that are localized to the refractive

structure of the eye lens. The protein encoded by this gene is unique in that it has both beta and gamma crystallin

protein motifs. (provided by RefSeq)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the CRYGN gene promoter:
         CUTL1   Nkx2-2   Hlf   NF-1   AREB6   NRSF form 1   NRSF form 2   Pax-6   FOXJ2 (long isoform)   c-Myc
         Other transcription factors

Search SABiosciences Chromatin IP Primers for CRYGN

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q36.1 Ensembl cytogenetic band: 7q36.1 HGNC cytogenetic band: 7q36.1

CRYGN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CRYGN gene location

GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure

GeneLoc location for GC07M144938: view genomic region (about GC identifiers)

Start:	144,938,635 bp from pter
End:	144,948,676 bp from pter
Size:	10,042 bases
Orientation:	minus strand

1 alternative location:

Chr7- 151,125,921-151,137,899

RefSeq DNA sequence:

NC_000007.13 NT_007914.15 NT_079596.2

(According to ¹UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
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UniProtKB/Swiss-Prot: CRGN_HUMAN, Q8WXF5 (See protein sequence)

Recommended Name: Gamma-crystallin N

Size: 182 amino acids; 20624 Da

Subunit: Monomer (By similarity)

Caution: In contrast to the orthologous protein found in other species, the C-terminal part differs and lacks the

fourth beta/gamma crystallin 'Greek key' domain. Moreover, its non-specific tissue specificity suggests that it may

have lost its function (PubMed:15853812)

Secondary accessions: Q496G6

Alternative splicing: 2 isoforms: Q8WXF5-1 Q8WXF5-2

Explore the universe of human proteins at neXtProt for CRYGN: NX_Q8WXF5

REFSEQ proteins: NP_653328.1

ENSEMBL proteins:

ENSP00000420157 ENSP00000338613

Human Recombinant Proteins

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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

CRYGN for domains About GeneDecksing

2 InterPro domains/families:

IPR001064 Beta/gamma_crystallin

IPR011024 G_crystallin-rel

Graphical View of Domain Structure for InterPro Entry Q8WXF5

ProtoNet protein and cluster: Q8WXF5

2 Blocks protein families:

IPB001064 Beta and gamma crystallin

IPB011024 Gamma-crystallin related

UniProtKB/Swiss-Prot: CRGN_HUMAN, Q8WXF5

Similarity: Belongs to the beta/gamma-crystallin family

Similarity: Contains 3 beta/gamma crystallin 'Greek key' domains

(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
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Cell
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SABiosciences Custom Pathway-Focused PCR Arrays for CRYGN

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for CRYGN

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse Small Molecules at Sigma-Aldrich

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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
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Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
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Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
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Clones:		Browse FLAG tag genes at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector: CRYGN OriGene Myc/DDK tagged cDNA clones in CMV expression vector: CRYGN OriGene untagged cDNA clones in CMV expression vector: CRYGN Browse 3'-UTR reporter clones for miRNA target validation Browse OriGene MicroRNA Expression Plasmids
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Primers:		OriGene genome-wide validated SYBR primer pairs: CRYGN Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for CRYGN: PPH19121A

REFSEQ mRNAs for CRYGN gene:

NM_144727.1

Additional cDNA sequence:

AF445455.1 BC100878.2 BC100879.3 BC100880.2 BC100881.2 EF491784.1

2 DOTS entries:

DT.97793177 DT.97807740

7 AceView cDNA sequences:

NM_144727 BU683519 AF445455 BC043605 BM548090 CB993791 BI003328

highest scoring ESTs for CRYGN:

AF445455 BC100878 BC100879 BC100880 BC100881 BM548090 CN290143 NM_144727 BC043605 CB993791

Unigene Cluster for CRYGN:

Crystallin, gamma N

Hs.647104 [show with all ESTs]

Unigene Representative Sequence: BM548090

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for CRYGN

ExUns:	1	^	2a	·	2b	^	3	^	4	^	5a	·	5b	·	5c
SP1:					-		-
SP2:							-		-
SP3:

About this scheme

ECgene alternative splicing isoforms for CRYGN
5 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000491928(uc003wkf.2) ENST00000476631(uc003wkg.2) ENST00000462809

ENST00000478106(uc010lqd.1) ENST00000337323(uc003wke.2)

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

CRYGN expression in normal and diseased human tissues

¹ / ²

1 probe-set matching CRYGN gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
1553082_at²	U133Plus2	1	0.82	1.00	--	--	--	--	--	--	--

About this table
Data from Genenote (Publications) and GNF BioGPS
About these images

CRYGN gene electronic northern expression

About these images

CGAP SAGE TAG: CCCTCAAAAA

SOURCE GeneReport for Unigene cluster: Hs.647104

UniProtKB/Swiss-Prot: CRGN_HUMAN, Q8WXF5

Tissue specificity: Not specifically expressed in eye

Primers:		OriGene genome-wide validated SYBR primer pairs: CRYGN Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for CRYGN: PPH19121A

SABiosciences Custom PCR Arrays for CRYGN

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for CRYGN gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	CRYGN¹	--	crystallin, gamma N	98.53(n) 97.8(a)	737768 XM_001141086.1 XP_001141086.1
cow (Bos taurus)	CRYGN¹	--	crystallin, gamma N	87.97(n) 88.72(a)	519041 XM_597249.3 XP_597249.3
mouse (Mus musculus)	Crygn⁵	5 (11.93 cM)	crystallin, gamma N	--	CF739670
zebrafish (Danio rerio)	Dr.29203²	--	Transcribed sequence with weak similarity to protein more	76.47(n)	CB359117.1
rainbow trout (Oncorhynchus mykiss)	Omy.13121²	--	Oncorhynchus mykiss transcribed sequences	78.07(n)	BX871985.1

About this table Species with no ortholog for CRYGN

ENSEMBL Gene Tree for CRYGN (if available)

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for CRYGN gene

CRYBG3² AIM1² AIM1L² CRYGS²

CRYGN for paralogs About GeneDecksing

(SNPs according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

SNP ID	Valid	Chr 7 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
Genomic Data				Transcription Related Data				Allele Frequencies
			--	--	--		--	--
rs2074999^1,2	C,F,	151135457(-)	`TGTGCA/GGCAGG`	1	--	int¹		12		EA NA NS WA	2226
rs2075000^1,2	C,F,	151133792(-)	`CAGAAC/TCCGGA`	1	--	int¹		15		EA NA NS	2336
rs2075001^1,2	C,F,	151133271(-)	`GGGGAC/TGGAGC`	2	D	ref¹ syn¹ ese³		13		EA NA NS	2184
rs2075002^1,2	C,F,	151132665(-)	`GCCCAG/AGCCCA`	1	--	int¹		12		EA NA NS WA	1582
rs2075003^1,2	C,F,A,H,	151129472(-)	`GCTCGA/GCCGGG`	1	--	int¹		2		EA WA	1472
rs4726025^1,2	C,A,H,	151129899(+)	`CTGCAC/TGCCCC`	1	--	int¹		5		NA EA WA	166
rs6464141^1,2	C,F,A,	151130452(+)	`ATGTGG/ATCCAA`	1	--	int¹		16		NS EA NA WA	1264
rs6965504^1,2	C,F,A,H,	151138704(+)	`CTTCTC/TAGgaa`	1	--	ng5¹		15		NS EA NA WA	1058
rs7808369^1,2	C,F,A,H,	151126822(+)	`tcagtG/Actcca`	1	--	ng3¹		7		NS EA NA WA	618
rs10226298^1,2	--	151137440(+)	`CTGCAA/GGTAGT`	1	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for CRYGN (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for CRYGN: --

Search QIAGEN SeqTarget long-range PCR primers for resequencing for CRYGN

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

CRYGN for disorders About GeneDecksing

OMIM: 609603

Export disorders and mutations for CRYGN gene to outside databases

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶UniProtKB/TrEMBL, and/or ⁷Novoseek)
About This Section

5 PubMed articles for CRYGN gene integrated from 7 sources:
(articles sorted by number of sources associating them with CRYGN)

Gamma-N-crystallin and the evolution of the betagamma-crystallin superfamily in vertebrates. (PubMed id 15853812)^{1, 2} Wistow G.... Vihtelic T. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)² Gerhard D.S....Malek J. (2004)
Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)¹ Scherer S.W.... Tsui L.-C. (2003)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)
Genetics of crystallins: cataract and beyond. (PubMed id 19007775)⁷ Graw J. (2009)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 155051	HGNC: 20458	AceView: CRYGN	Ensembl:ENSG00000127377	euGenes: HUgn155051
ECgene: CRYGN	H-InvDB: CRYGN

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Intellectual Property for CRYGN gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from XenneX, Inc.)

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Patent Information for CRYGN gene:
Search GeneIP for patents involving CRYGN

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
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