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CYP4Z1 Gene

protein-coding   GIFtS: 42

GC01P045649
cytochrome P450, family 4, subfamily Z, polypeptide 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases & Descriptions
cytochrome P450, family 4, subfamily Z, polypeptide 11 2
CYP4A201 2
CYPIVZ12 3
cytochrome P450 4Z12
EC 1.14.14.13

External Ids:    HGNC: 205831   Entrez Gene: 1999742   Ensembl: ENSG000001861607   UniProtKB: Q86W103   

Export aliases for CYP4Z1 gene to outside databases

Previous GC identifers: GC01P046923 GC01P046903 GC01P046902 GC01P047245 GC01P047305 GC01P047533


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CYP4Z1:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are
monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and
other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 1p33. (provided by RefSeq)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CYP4Z1 gene promoter:
         NF-Y   Chx10   NF-YA   CP1A   Meis-1   HOXA9B   HNF-1A   XBP-1   AP-2rep   CUTL1   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for CYP4Z1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p33   Ensembl cytogenetic band:  1p33   HGNC cytogenetic band: 1p33

CYP4Z1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CYP4Z1 gene location

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P045649:  view genomic region     (about GC identifiers)

Start:
45,649,119 bp from pter
End:
45,699,646 bp from pter
Size:
50,528 bases
Orientation:
plus strand

1 alternative location:
Chr1+ 47,533,160-47,583,991     
RefSeq DNA sequence:
NC_000001.10  NT_032977.9  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
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UniProtKB/Swiss-Prot: CP4Z1_HUMAN, Q86W10 (See protein sequence)
Recommended Name: Cytochrome P450 4Z1  
Size: 505 amino acids; 59086 Da
Cofactor: Heme group (By similarity)
Subcellular location: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Microsome membrane;
Single-pass type II membrane protein
Secondary accessions: Q5VVE4

Explore the universe of human proteins at neXtProt for CYP4Z1: NX_Q86W10 

REFSEQ proteins: NP_835235.1  

ENSEMBL proteins: 
ENSP00000334246 


Human Recombinant Proteins 
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5 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005783 endoplasmic reticulum IEA--
GO:0005789 endoplasmic reticulum membrane IEA--
GO:0005792 microsome IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

CYP4Z1 for ontologies           About GeneDecksing



Antibodies for CYP4Z1: 
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Uscn ELISAs and CLIAs for CYP4Z1 


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

CYP4Z1 for domains           About GeneDecksing

3 InterPro domains/families:
 IPR001128 Cyt_P450
 IPR017972 Cyt_P450_CS
 IPR002401 Cyt_P450_E_grp-I

Graphical View of Domain Structure for InterPro Entry Q86W10

ProtoNet protein and cluster: Q86W10

UniProtKB/Swiss-Prot: CP4Z1_HUMAN, Q86W10
Similarity: Belongs to the cytochrome P450 family


(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: CP4Z1_HUMAN, Q86W10
Catalytic activity: RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O
Enzyme Number (IUBMB): EC 1.14.14.1 

Inhib.
RNA:
    
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich siRNA Panels and esiRNA and siRNA for CYP4Z1
Sigma-Aldrich shRNA for CYP4Z1
OriGene 29mer shRNA kits in GFP-retroviral vector: CYP4Z1
OriGene shRNA RFP: CYP4Z1
OriGene basic RS shRNA: CYP4Z1
OriGene siRNA: CYP4Z1
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of CYP4Z1 
miRNA:Browse microRNA Mimics at Sigma-Aldrich
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of CYP4Z1 
Search SABiosciences for Assays for microRNAs that regulate CYP4Z1

Gene
Editing:
Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: CYP4Z1
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: CYP4Z1
OriGene untagged cDNA clones in CMV expression vector: CYP4Z1
OriGene 3'-UTR clone: CYP4Z1 
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GenScript cDNA clone with any tag delivered in your preferred vector: CYP4Z1 
Browse Sino Biological Human cDNA Clones

Cell
Lines:
  
GenScript Custom overexpressing Cell Line Services for CYP4Z1 

5/6 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 6):

GO IDQualified GO termEvidencePubMed IDs
GO:0004497 monooxygenase activity IEA--
GO:0005506 iron ion binding ----
GO:0009055 electron carrier activity IEA--
GO:0020037 heme binding IEA--
GO:0046872 metal ion binding IEA--
About this table

CYP4Z1 for ontologies           About GeneDecksing


(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section


CYP4Z1 for pathways           About GeneDecksing

5/6 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  CYP4Z1 (see all 6)
    Arachidonic Acid Metabolism
Fatty Acid Metabolism
NRF2-mediated Oxidative Stress Response
Metabolism of Xenobiotics by Cytochrome P450
Tryptophan Metabolism

    SABiosciences Custom Pathway-Focused PCR Arrays for CYP4Z1 

    Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for CYP4Z1
    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CYP4Z1

1 Gene Ontology (GO) biological process term (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0055114 oxidation-reduction process IEA--
About this table

CYP4Z1 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for CYP4Z1


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib.
RNA:
     
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich siRNA Panels and esiRNA and siRNA for CYP4Z1
Sigma-Aldrich shRNA for CYP4Z1
OriGene 29mer shRNA kits in GFP-retroviral vector: CYP4Z1
OriGene shRNA RFP: CYP4Z1
OriGene basic RS shRNA: CYP4Z1
OriGene siRNA: CYP4Z1
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of CYP4Z1 
miRNA: Browse microRNA Mimics at Sigma-Aldrich
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of CYP4Z1 
Search SABiosciences for Assays for microRNAs that regulate CYP4Z1
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: CYP4Z1
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: CYP4Z1
OriGene untagged cDNA clones in CMV expression vector: CYP4Z1
OriGene 3'-UTR Clone: CYP4Z1 
Browse OriGene MicroRNA Expression Plasmids 
GenScript cDNA clone with any tag delivered in your preferred vector: CYP4Z1 
Primers: OriGene genome-wide validated SYBR primer pairs: CYP4Z1
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for CYP4Z1: PPH13648A

REFSEQ mRNAs for CYP4Z1 gene: 

NM_178134.2  

Additional cDNA sequence: 

AK292175.1 AY262056.1 AY358631.1 

2 DOTS entries:

DT.100742965  DT.106617 

22 AceView cDNA sequences:

AY358631 NM_178134 BG218514 BG193485 AY262056 BX094680 BG210988 H21976 
AI733538 BG201072 AI668594 AV700083 AV689946 BG219391 H25577 BG212152 
AI668602 AW472756 BG192933 AI820775 BG204180 H25624 

highest scoring ESTs for CYP4Z1:

AI668602 AY262056 AY358631 AI668594 AI733538 AI820775 AV700083 AW472756 BG192933 BG193485 

Unigene Cluster for CYP4Z1:

Cytochrome P450, family 4, subfamily Z, polypeptide 1
Hs.176588  [show with all ESTs]
Unigene Representative Sequence: AY358631


GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000334194(uc001cqu.1) ENST00000471598

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

CYP4Z1 expression in normal and diseased human tissues

1  / 2  / 3

3 probe-sets matching CYP4Z1 gene

Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
59570_at2, 3 U95-B 2 0.94 0.97 1.00 1.00 AI668602 0.80 1.00 0.91 1
237395_at2, 3 U133-B 2 1.00 0.95 -- -- AV700083 0.60 1.00 0.82 1
237395_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
About this table

CYP4Z1 for expression           About GeneDecksing

Data from Genenote  (Publications) and GNF BioGPS
    About these images
CYP4Z1 gene expression
CYP4Z1 gene electronic northern expression
CYP4Z1 gene sage expression
About these images

CGAP SAGE TAG: ATAGCTTTAT

SOURCE GeneReport for Unigene cluster: Hs.176588

UniProtKB/Swiss-Prot: CP4Z1_HUMAN, Q86W10
Tissue specificity: Preferentially detected in breast carcinoma tissue and mammary gland, whereas only marginal
expression is found in all other tested tissues

Primers: OriGene genome-wide validated SYBR primer pairs: CYP4Z1
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for CYP4Z1: PPH13648A
    SABiosciences Custom PCR Arrays for CYP4Z1 


(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for CYP4Z1 gene from 3 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
African clawed frog
(Xenopus laevis)
AB022671.12   --   -- 73.56(n)  AB022671.1 
thale cress
(Arabidopsis thaliana)
BAS1/CYP734A11   -- BAS1/CYP734A1 (PHYB ACTIVATION TAGGED SUPPRESSOR 1); more 44.55(n)
33.65(a)
817212  NM_128228.2  NP_180239.1 
rice
(Oryza sativa)
Os06g06004001   -- hypothetical protein 42.5(n)
30.17(a)
4341450  NM_001064535.1  NP_001058000.1 
About this table        Species with no ortholog for CYP4Z1

ENSEMBL Gene Tree for CYP4Z1 (if available)

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
Paralogs for CYP4Z1 gene
CYP4B12  CYP4A112  CYP4X12  CYP4A222  

CYP4Z1 for paralogs           About GeneDecksing



(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

10/426 NCBI SNPs in CYP4Z1 are shown (see all 426)
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
----------
rs10023781,2
C,F,A,H47545312(+) AACAAG/ATAGAT 1 -- int182Minor allele frequency- A:0.08NS EA PA CA WA 4020
rs11104251,2
C,A,47571536(+) TGATAT/CTGGTC 1 -- int14Minor allele frequency- C:0.38NA EA 186
rs12954621,2
--47575540(-) GGCCAT/CGTAGA 1 -- int11Minor allele frequency- C:0.00MN 184
rs12954631,2
C,A,H,47582246(+) GATTTT/CGTTTT 1 -- int13Minor allele frequency- C:0.17NA WA 6
rs15029141,2
--47533432(+) aatatG/Tcaaaa 1 -- int10--------
rs15029241,2
C,O,A,H,47547972(+) GGCCTG/CTGATA 1 -- int11Minor allele frequency- C:0.00NA 2
rs15029271,2
C,H,47548670(+) ACACAG/ATTGTG 1 -- int19Minor allele frequency- A:0.00NS EA NA WA 430
rs18472201,2
--47551460(-) tgggtA/Gcaaca 1 -- int10--------
rs18472211,2
C,A,H,47576691(-) ACTGGG/AACACC 1 -- int13Minor allele frequency- A:0.32MN NA 190
rs18472221,2
C,A,H47572843(-) gttaaC/Tacatg 1 -- int12Minor allele frequency- T:0.50NA WA 4
About this table

HapMap Linkage Disequilibrium images for CYP4Z1 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for CYP4Z1
     1 CNV: 68525

QIAGEN SeqTarget long-range PCR primers for resequencing CYP4Z1 

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section
  --

(Possibly Related Articles in Doctor's Guide)
About This Section
  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

10 PubMed articles for CYP4Z1 gene integrated from 7 sources:
(articles sorted by number of sources associating them with CYP4Z1)
  1. Identification of a novel mammary-restricted cytochrome P450, CYP4Z1, with overexpression in breast carcinoma. (PubMed id 15059886)1, 2, 3, 7 Rieger M.A.... Weigle B. (2004)
  2. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
  3. Conditional regulation of the human CYP4X1 and CYP4Z1 genes. (PubMed id 15797250)1, 7 Savas U....Johnson E.F. (2005)
  4. Identification of genes associated with non-small-cel l lung cancer promotion and progression. (PubMed id 19473719)1 Bankovic J....Tanic N. (2010)
  5. Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid. (PubMed id 19090726)1 ZAPllner A....Bureik M. (2009)
  6. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)2 Gregory S.G.... Bentley D.R. (2006)
  7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
  8. Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants. (PubMed id 15128046)1 Nelson D.R....Nebert D.W. (2004)
  9. The cytochrome P450 4 (CYP4) family. (PubMed id 9068972)1 Simpson A.E. (1997)
  10. Cytochrome P450 expression and metabolic activation of cooked food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in MCF10A breast epithelial cells. (PubMed id 16527260)7 Thomas R.D....Runge-Morris M. (2006)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 199974 HGNC: 20583 AceView: CYP4Z1 Ensembl:ENSG00000186160 euGenes: HUgn199974
ECgene: CYP4Z1 H-InvDB: CYP4Z1

(According to HUGE)
About This Section
  --

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
  --

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for CYP4Z1 gene:
Search GeneIP for patents involving CYP4Z1

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

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 "Your Favorite Gene" Pathways and Molecular Interaction Networks Browse Nano Scale siRNA at Sigma-Aldrich
 Browse iPSC Reprogramming Factors at Sigma-Aldrich Browse small molecules at Sigma-Aldrich
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The GeneCards human gene database gene index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

CYP4Z1 gene at Home site.
hostname: www-ab3 db genecards_305 index build: 81e solr: 1.4