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GPR182 Gene

protein-coding   GIFtS: 45

GC12P054428
G protein-coupled receptor 182
(Previous name: adrenomedullin receptor )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: ADMR)
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
G protein-coupled receptor 1821 2     AMR2
AM-R1 2     7TMR2
adrenomedullin receptor1 2     gamrh2
hrhAMR1 2     MGC343992
G10D1 2     G-protein coupled receptor 1822
ADMR2 3     

External Ids:    HGNC: 137081   Entrez Gene: 113182   Ensembl: ENSG000001668567   UniProtKB: O152183   

Export aliases for GPR182 gene to outside databases

Previous GC identifers: GC12P055675 GC12P057388


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GPR182:
Adrenomedullin is a potent vasodilator peptide that exerts major effects on cardiovascular function. This gene encodes
a seven-transmembrane protein that belongs to the family 1 of G-protein coupled receptors. Studies of the rat
counterpart suggest that the encoded protein may function as a receptor for adrenomedullin. (provided by RefSeq)

UniProtKB/Swiss-Prot: GP182_HUMAN, O15218
Function: Orphan receptor

Gene Wiki entry for GPR182

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for GPR182
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for GPR182

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.3   Ensembl cytogenetic band:  12q13.3   HGNC cytogenetic band: 12q13.3

GPR182 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPR182 gene location

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P054428:  view genomic region     (about GC identifiers)

Start:
54,427,154 bp from pter
End:
54,444,914 bp from pter
Size:
17,761 bases
Orientation:
plus strand

1 alternative location:
Chr12+ 57,388,230-57,390,468     
RefSeq DNA sequence:
NC_000012.11  NT_029419.12  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: GP182_HUMAN, O15218 (See protein sequence)
Recommended Name: G-protein coupled receptor 182  
Size: 404 amino acids; 45323 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Caution: Was originally (PubMed:9367907) thought to be a receptor for adrenomedullin

Explore the universe of human proteins at neXtProt for GPR182: NX_O15218 

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_009195.1  

    ENSEMBL proteins: 
    ENSP00000300098 


    Human Recombinant Proteins 
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    Novus Biologicals Lysate for GPR182
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    2 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    GPR182 for ontologies           About GeneDecksing



    Antibodies for GPR182: 
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    Browse ELISAs and CLIAs at Uscn 


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    GPR182 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR017452 GPCR_Rhodpsn_supfam
     IPR001350 G10D_rcpt
     IPR000276 7TM_GPCR_Rhodpsn

    Graphical View of Domain Structure for InterPro Entry O15218

    ProtoNet protein and cluster: O15218

    1 Blocks protein family: IPB001350 G10D orphan receptor signature

    UniProtKB/Swiss-Prot: GP182_HUMAN, O15218
    Similarity: Belongs to the G-protein coupled receptor 1 family


    (According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
    About This Section

    UniProtKB/Swiss-Prot: GP182_HUMAN, O15218
    Function: Orphan receptor

    Inhib.
    RNA:
        
    Browse for Gene Knock-down Tools from Millipore
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    miRNA:Browse microRNA Mimics at Sigma-Aldrich
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPR182 
    Search SABiosciences for Assays for microRNAs that regulate GPR182

    Gene
    Editing:
    Browse CompoZr Knockout ZFN at Sigma-Aldrich 

    Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
    Browse iPSC Reprogramming Factors at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: GPR182
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPR182
    OriGene untagged cDNA clones in CMV expression vector: GPR182
    OriGene 3'-UTR clone: GPR182 
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    Browse Sino Biological Human cDNA Clones

    Cell
    Lines:
      
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    3 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001605 adrenomedullin receptor activity IEA--
    GO:0004888 transmembrane receptor activity TAS9367907
    GO:0004930 G-protein coupled receptor activity IEA--
    About this table

    GPR182 for ontologies           About GeneDecksing


    (Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
    About This Section


    GPR182 for pathways           About GeneDecksing

    1 Millipore Pathway for GPR182
        Adenylate cyclase-activating neuropeptides

        SABiosciences Pathway-Focused PCR Array including GPR182: PAHS-3009A 

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPR182

    3 Gene Ontology (GO) biological process terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001666 response to hypoxia IEA--
    GO:0007166 cell surface receptor linked signaling pathway TAS9367907
    GO:0007186 G-protein coupled receptor protein signaling pathway ----
    About this table

    GPR182 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    GPR182 for compounds           About GeneDecksing

    Browse Small Molecules at Sigma-Aldrich
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for GPR182
    4 Novoseek chemical compound relationships for GPR182 gene
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    bibn 4096 bs 79.1 2 12970090 (1), 12183333 (1)
    adenylate 29.2 1 7621913 (1)
    glyceraldehyde 3-phosphate 20 1 11905404 (1)
    tyrosine 0 1 16267056 (1)
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Millipore,
    siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
    Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
    About This Section

    Inhib.
    RNA:
         
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich siRNA Panels and esiRNA and siRNA for GPR182
    Sigma-Aldrich shRNA Panels and shRNA for GPR182
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    OriGene basic RS shRNA: GPR182
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    miRNA: Browse microRNA Mimics at Sigma-Aldrich
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPR182 
    Search SABiosciences for Assays for microRNAs that regulate GPR182
    Clones: Browse FLAG tag genes at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: GPR182
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPR182
    OriGene untagged cDNA clones in CMV expression vector: GPR182
    OriGene 3'-UTR Clone: GPR182 
    Browse OriGene MicroRNA Expression Plasmids 
    GenScript cDNA clone with any tag delivered in your preferred vector: GPR182 
    Primers: OriGene genome-wide validated SYBR primer pairs: GPR182
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for GPR182: PPH08112A

    REFSEQ mRNAs for GPR182 gene: 

    NM_007264.3  

    Additional cDNA sequence: 

    BC034761.1 Y13583.1 

    2 DOTS entries:

    DT.92416997  DT.121199001 

    18 AceView cDNA sequences:

    BM919720 NM_007264 BC034761 AI433867 BI759854 AI962628 AI379917 BX114393 
    CD246170 BM091055 BI830122 AW135380 AI243295 AA704594 Y13583 BM091360 
    T90363 BP340154 

    Unigene Cluster for GPR182:

    G protein-coupled receptor 182
    Hs.483909  [show with all ESTs]
    Unigene Representative Sequence: BC034761


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000300098(uc001smk.2)

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Primers from OriGene and/or SABiosciences )
    About This Section

    GPR182 expression in normal and diseased human tissues

    1  / 2

    4 probe-sets matching GPR182 gene

    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    36429_at2 U95-A 1 0.88 1.00 1.00 1.00 -- -- -- -- --
    214506_at2 U133-A 1 0.36 1.00 -- -- -- -- -- -- --
    214506_at2 U133Plus2 1 0.36 1.00 -- -- -- -- -- -- --
    1552440_at2 U133Plus2 1 0.36 1.00 -- -- -- -- -- -- --
    About this table

    GPR182 for expression           About GeneDecksing

    Data from Genenote  (Publications) and GNF BioGPS
        About these images
    GPR182 gene expression
    GPR182 gene electronic northern expression
    GPR182 gene sage expression
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.483909

    UniProtKB/Swiss-Prot: GP182_HUMAN, O15218
    Tissue specificity: Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver

    Primers: OriGene genome-wide validated SYBR primer pairs: GPR182
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for GPR182: PPH08112A
        SABiosciences Expression via Pathway-Focused PCR Array including GPR182: PAHS-3009A 


    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section

    Orthologs for GPR182 gene from 5/7 species (see all 7)
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    GPR1821   -- G protein-coupled receptor 182 78.28(n)
    70.21(a)
    481120  XM_538241.1  XP_538241.1 
    cow
    (Bos taurus)
    GPR1821   -- G protein-coupled receptor 182 85.64(n)
    80.16(a)
    509554  XM_586542.3  XP_586542.2 
    rat
    (Rattus norvegicus)
    Gpr1821   -- G protein-coupled receptor 182 81.03(n)
    74.87(a)
    29307  NM_053302.2  NP_445754.2 
    mouse
    (Mus musculus)
    Gpr1821 , 5 10 (75.07 cM)5
    G protein-coupled receptor 1821, 5 80.74(n)1
    74.09(a)1
    115361  NM_007412.21  NP_031438.21 
     AF0012295  AK0314805  (see all 14)
    zebrafish
    (Danio rerio)
    gpr1821   -- G protein-coupled receptor 182 53.03(n)
    41.79(a)
    323641  NM_001020478.1  NP_001018314.1 
    About this table        Species with no ortholog for GPR182

    ENSEMBL Gene Tree for GPR182 (if available)

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section
    Paralogs for GPR182 gene
    CXCR72  XCR12  CX3CR12  CCBP22  

    GPR182 for paralogs           About GeneDecksing



    (SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
    About This Section

    10/38 NCBI SNPs in GPR182 are shown (see all 38)
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ----------
    rs4076361,2
    --57387289(+) CTCCAC/GAATCC 1 -- ng510--------
    rs4235271,2
    C57387293(-) CGTTGG/TATTGT 1 -- ng510--------
    rs22793731,2
    C,F,57388198(+) CACACC/TGGGTC 1 -- ng518Minor allele frequency- T:0.12NS EA NA WA 628
    rs37415791,2
    --57389245(-) CCTGCC/TCGGCC 2 R syn1 ref1 ese30--------
    rs120996951,2
    C,F,57388569(+) CTCACG/AGTCCA 1 -- int13Minor allele frequency- A:0.12NA EA WA 210
    rs128095091,2
    --57388897(+) GCCCCC/GGACTC 1 -- int10--------
    rs343776471,2
    C,F,57389623(+) GAAACG/ATACAG 2 /T syn1 ref13Minor allele frequency- A:0.10NA WA 128
    rs346463511,2
    --57390286(+) GTAGA-/GGGGGA 1 -- ut310--------
    rs353035261,2
    C57389212(+) CTCCTG/AGTGAT 2 /L syn1 ref11Minor allele frequency- A:0.01NA 74
    rs354931211,2
    C,F,57390038(+) GCACAT/CGCGCC 2 /R /C mis1 ref18Minor allele frequency- C:0.11NA NS EA WA 656
    About this table

    HapMap Linkage Disequilibrium images for GPR182 (up to first 250kb)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for GPR182: --

    QIAGEN SeqTarget long-range PCR primers for resequencing GPR182 

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    GPR182 for disorders           About GeneDecksing

    OMIM: 605307

    8 Novoseek disease relationships for GPR182 gene

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    hypertension renal 47.4 2 9120666 (1), 8951571 (1)
    heart failure congestive 8.94 2 9120666 (1), 8951571 (1)
    sepsis 7.1 1 11997081 (1)
    tumors 3.47 3 9016306 (1), 17363587 (1), 15245870 (1)
    cardiovascular diseases 0 1 15273827 (1)
    renal failure 0 2 9120666 (1), 8951571 (1)
    pulmonary disease chronic obstructive 0 5 14720432 (2), 12877796 (1)
    heart failure 0 1 12630824 (1)
    About this table


    Export disorders and mutations for GPR182 gene to outside databases

    (Possibly Related Articles in Doctor's Guide)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
    About This Section

    10/97 PubMed articles for GPR182 gene, integrated from 7 sources (see all 97):
    (articles sorted by number of sources associating them with GPR182)
    1. Expression of the rat adrenomedullin receptor or a putative human adrenomedullin receptor does not correlate with adrenomedullin binding or functional response. (PubMed id 9535752)1, 2, 3, 7 Kennedy S.P.... Hill R.J. (1998)
    2. Molecular cloning of a novel human receptor gene with homology to the rat adrenomedullin receptor and high expression in heart and immune system. (PubMed id 9367907)1, 2, 3, 7 Haenze J.... Rascher W. (1997)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Localization and distribution of adrenomedullin receptor in the human placenta: changes with gestational age. (PubMed id 17939601)1, 7 Marinoni E....Di Iorio R. (2007)
    5. [Study of the expression and role of adrenomedullin and adrenomedullin receptor in patients with chronic obstructive pulmonary disease] (PubMed id 14720432)1, 7 Xu P....Song W.D. (2003)
    6. Adrenomedullin receptor expression in human lung and in pulmonary tumors. (PubMed id 9016306)1, 7 Martinez A....Cuttitta F. (1997)
    7. The cardiovascular response in sepsis: proposed mechanisms of the beneficial effect of adrenomedullin and its binding protein (review). (PubMed id 11956648)1, 7 Fowler D.E. and Wang P. (2002)
    8. Genomic organization and regulation of a human 7-helix transmembrane receptor which is expressed in pulmonary epithelial cells and induced in hypoxia. (PubMed id 11883938)1, 7 Haenze J.... Fischer A. (2002)
    9. Receptors for adrenomedullin in human vascular endothelial cells. (PubMed id 7621913)1, 7 Kato J....Eto T. (1995)
    10. The ADMR receptor mediates the effects of adrenomedul lin on pancreatic cancer cells and on cells of the tumor microenvironment. (PubMed id 19847298)1 Ramachandran V....Logsdon C.D. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 11318 HGNC: 13708 AceView: ADMR Ensembl:ENSG00000166856 euGenes: HUgn11318
    ECgene: GPR182 H-InvDB: GPR182

    (According to HUGE)
    About This Section
      --

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
      --

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from XenneX, Inc.)
    About This Section
    Patent Information for GPR182 gene:
    Search GeneIP for patents involving GPR182

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
    Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
    About This Section

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     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPR182
     SeqTarget long-range PCR primers for resequencing GPR182
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPR182
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    GeneCards Homepage - Last full update: 31 Dec 2010 - Incremental updates: 9 Jan 2011 , 10 Jan 2011

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