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GPX6 Gene

protein-coding   GIFtS: 48

GC06M028277
glutathione peroxidase 6 (olfactory)
(Previous name: glutathione peroxidase pseudogene 3 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: GPXP3)
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
glutathione peroxidase 6 (olfactory)1 2     glutathione peroxidase 62
glutathione peroxidase pseudogene 31 2     GPX5p2
GPx-62 3     dJ1186N242
GSHPx-62 3     dJ1186N24.12
GPXP32     EC 1.11.1.93

External Ids:    HGNC: 45581   Entrez Gene: 2572022   Ensembl: ENSG000001987047   UniProtKB: P597963   

Export aliases for GPX6 gene to outside databases

Previous GC identifers: GC00U990129 GC06M028541 GC06P028601 GC06M028579 GC06M028471


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GPX6:
This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen
peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA
codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop
structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather
than as a stop signal. Expression of this gene is restricted to embryos and adult olfactory epithelium. (provided by
RefSeq)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GPX6 gene promoter:
         C/EBPbeta   SRF   c-Rel   ARP-1   MRF-2   FOXO4   PPAR-gamma1   PPAR-gamma2   HSF2   RelA   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for GPX6

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6p22.1   Ensembl cytogenetic band:  6p22.1   HGNC cytogenetic band: 6p22.1

GPX6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPX6 gene location

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M028277:  view genomic region     (about GC identifiers)

Start:
28,277,455 bp from pter
End:
28,289,954 bp from pter
Size:
12,500 bases
Orientation:
minus strand

2 alternative locations:
Chr6-,ALT_REF_LOCI_2 28,517,716-28,522,758      Chr6- 28,471,073-28,503,695     
RefSeq DNA sequence:
NC_000006.11  NT_007592.15  NT_113891.2  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: GPX6_HUMAN, P59796 (See protein sequence)
Recommended Name: Glutathione peroxidase 6 precursor  
Size: 221 amino acids; 24971 Da
Subcellular location: Secreted (By similarity)
Secondary accessions: Q4PJ17

Explore the universe of human proteins at neXtProt for GPX6: NX_P59796 

REFSEQ proteins: NP_874360.1  

ENSEMBL proteins: 
ENSP00000354581 ENSP00000417364 ENSP00000382223 


Human Recombinant Proteins 
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1 Gene Ontology (GO) cellular component term (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
About this table

GPX6 for ontologies           About GeneDecksing



Antibodies for GPX6: 
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Uscn ELISAs and CLIAs for GPX6 


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

GPX6 for domains           About GeneDecksing

3 InterPro domains/families:
 IPR012336 Thioredoxin-like_fold
 IPR012335 Thioredoxin_fold
 IPR000889 Glutathione_peroxidase

Graphical View of Domain Structure for InterPro Entry P59796

ProtoNet protein and cluster: P59796

1 Blocks protein family: IPB000889 Glutathione peroxidase

UniProtKB/Swiss-Prot: GPX6_HUMAN, P59796
Similarity: Belongs to the glutathione peroxidase family


(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: GPX6_HUMAN, P59796
Catalytic activity: 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Enzyme Number (IUBMB): EC 1.11.1.9 

Inhib.
RNA:
    
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich siRNA Panels and siRNA for GPX6
Sigma-Aldrich shRNA for GPX6
OriGene 29mer shRNA kits in GFP-retroviral vector: GPX6
OriGene shRNA RFP: GPX6
OriGene basic RS shRNA: GPX6
OriGene siRNA: GPX6
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX6 
miRNA:Sigma-Aldrich microRNA Mimics for GPX6
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX6 
Search SABiosciences for Assays for microRNAs that regulate GPX6

Gene
Editing:
Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: GPX6
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPX6
OriGene untagged cDNA clones in CMV expression vector: GPX6
Browse 3'-UTR reporter clones for miRNA target validation 
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GenScript cDNA clone with any tag delivered in your preferred vector: GPX6 
Browse Sino Biological Human cDNA Clones

Cell
Lines:
  
GenScript Custom overexpressing Cell Line Services for GPX6 

2 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004602 glutathione peroxidase activity IEA--
GO:0016491 oxidoreductase activity IEA--
About this table

GPX6 for ontologies           About GeneDecksing


(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section


GPX6 for pathways           About GeneDecksing

1 Millipore Pathway for GPX6
    Glutathione metabolism

2 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  GPX6
    Arachidonic Acid Metabolism
Glutathione Metabolism

2 Kegg Pathways  (Kegg details for GPX6):
    hsa00480 Glutathione metabolism
hsa00590 Arachidonic acid metabolism

    SABiosciences Pathway-Focused PCR Arrays including GPX6 (see all 3): PAHS-062A PAHS-065A  

    Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for GPX6
    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPX6

5/36 Interacting proteins for GPX6 (ENSP000003545813) via UniProtKB, MINT, and/or STRING (see all 36)
InteractantInteraction Details
GeneCardExternal ID(s)
ALOX5ENSP000003635123STRING: ENSP00000363512
GGT1ENSP000002489233STRING: ENSP00000248923
GSSENSP000002169513STRING: ENSP00000216951
GSTA1ENSP000003356203STRING: ENSP00000335620
GSTA3ENSP000002111223STRING: ENSP00000211122
About this table

2 Gene Ontology (GO) biological process terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006979 response to oxidative stress IEA--
GO:0055114 oxidation-reduction process IEA--
About this table

GPX6 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for GPX6


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib.
RNA:
     
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich siRNA Panels and siRNA for GPX6
Sigma-Aldrich shRNA for GPX6
OriGene 29mer shRNA kits in GFP-retroviral vector: GPX6
OriGene shRNA RFP: GPX6
OriGene basic RS shRNA: GPX6
OriGene siRNA: GPX6
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX6 
miRNA: Sigma-Aldrich microRNA Mimics for GPX6
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX6 
Search SABiosciences for Assays for microRNAs that regulate GPX6
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: GPX6
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPX6
OriGene untagged cDNA clones in CMV expression vector: GPX6
Browse 3'-UTR reporter clones for miRNA target validation 
Browse OriGene MicroRNA Expression Plasmids 
GenScript cDNA clone with any tag delivered in your preferred vector: GPX6 
Primers:Browse OriGene genome-wide validated SYBR primer pairs
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for GPX6: PPH06081A

REFSEQ mRNAs for GPX6 gene: 

NM_182701.1  

Additional cDNA sequence: 

AY324826.1 BC133691.1 BC148315.2 

1 DOTS entry:

DT.101977842 

2 AceView cDNA sequences:

NM_182701 AY324826 

highest scoring ESTs for GPX6:

AY324826 NM_182701 DB034136 DB337290 

Unigene Cluster for GPX6:

Glutathione peroxidase 6 (olfactory)
Hs.448570
Unigene Representative Sequence: AY324826


GeneLoc Exon Structure

4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000361902(uc011dlj.1 uc010jrg.1) ENST00000474923 ENST00000483058
ENST00000399282

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

GPX6 expression in normal and diseased human tissues

1 Data from GNF BioGPS
    About these images

GPX6 gene expression
GPX6 gene electronic northern expression
GPX6 gene sage expression
About these images

CGAP SAGE TAG: GCACCTGGGC

SOURCE GeneReport for Unigene cluster: Hs.448570

UniProtKB/Swiss-Prot: GPX6_HUMAN, P59796
Tissue specificity: Expressed in olfactory epithelium and embryos

Primers:Browse OriGene genome-wide validated SYBR primer pairs
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for GPX6: PPH06081A
    SABiosciences Expression via Pathway-Focused PCR Arrays including GPX6 (see all 3): PAHS-062A PAHS-065A  


(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for GPX6 gene from 3 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
rat
(Rattus norvegicus)
Gpx61   -- glutathione peroxidase 6 79.7(n)
73.64(a)
259233  NM_147165.1  NP_671694.1 
mouse
(Mus musculus)
Gpx61 , 5 13 (7.74 cM)5
glutathione peroxidase 61, 5 80.61(n)1
71.36(a)1
755121  NM_145451.21  NP_663426.11 
 AA0682035  AK0061615  (see all 10)
worm
(Caenorhabditis elegans)
C11E4.21   -- hypothetical protein 50.97(n)
41.94(a)
182513  NM_077215.2  NP_509616.1 
About this table        Species with no ortholog for GPX6

ENSEMBL Gene Tree for GPX6 (if available)

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
Paralogs for GPX6 gene
GPX52  GPX22  GPX42  GPX72  GPX12  GPX82  GPX32  

GPX6 for paralogs           About GeneDecksing



(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

10/251 NCBI SNPs in GPX6 are shown (see all 251)
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
----------
rs3859081,2
C,F28481418(+) CAAGCA/CTGCCA 1 -- int16Minor allele frequency- C:0.03NS WA 180
rs4009871,2
C,F28480931(+) ttccaC/Tttaat 1 -- int16Minor allele frequency- T:0.04NS WA 174
rs4037741,2
C,F28485279(+) CCACAG/AATAGA 1 -- ng516Minor allele frequency- A:0.03NS WA 182
rs4061131,2
C,F,A,H28483482(+) AGGAAC/AAACAG 2 /L /F mis1 ref1 ese336Minor allele frequency- A:0.48NS EA NA MN WA 4096
rs4341121,2
C,F,A28480833(+) CACACC/TGGAAG 1 -- int15Minor allele frequency- T:0.30NA WA 10
rs7227881,2
C,F,A,H28483150(+) GGGCCA/CCCAGG 1 -- int18Minor allele frequency- C:0.15NS NA 194
rs9743341,2
C,F,O,A,H,28474218(+) GTGGAC/GAATGA 1 -- int122Minor allele frequency- G:0.43MN EA NS NA WA 2699
rs20719661,2
C,F,28473325(+) TCTACT/GTAAAG 1 -- int115Minor allele frequency- G:0.35EA NS NA WA 2304
rs20784221,2
C,F,A,H,28476497(+) gagaaT/Cagctt 1 -- int16Minor allele frequency- C:0.03NS WA 182
rs20784391,2
C,F,A28482967(+) CAGATC/TGCAAG 1 -- int117Minor allele frequency- T:0.32NA EA NS WA 1109
About this table

HapMap Linkage Disequilibrium images for GPX6 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for GPX6
     1 CNV: 81297

QIAGEN SeqTarget long-range PCR primers for resequencing GPX6 

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

GPX6 for disorders           About GeneDecksing

OMIM: 607913

Human Genome Epidemiology (HuGE) Navigator: GPX6 (4 documents)

Export disorders and mutations for GPX6 gene to outside databases

(Possibly Related Articles in Doctor's Guide)
About This Section
  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

10 PubMed articles for GPX6 gene integrated from 7 sources:
(articles sorted by number of sources associating them with GPX6)
  1. Characterization of mammalian selenoproteomes. (PubMed id 12775843)1, 2 Kryukov G.V.... Gladyshev V.N. (2003)
  2. Glutathione pathway genetic polymorphisms and lung ca ncer survival after platinum-based chemotherapy. (PubMed id 20200426)1 Moyer A.M....Weinshilboum R.M. (2010)
  3. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1 Bailey S.D....Anand S. (2010)
  4. Gene-centric association signals for lipids and apoli poproteins identified via the HumanCVD BeadChip. (PubMed id 19913121)1 Talmud P.J.... . (2009)
  5. HIV-1 viral proteins gp120 and Tat induce oxidative stress in brain endothelial cells. (PubMed id 15910762)1 Price T.O....Banks W.A. (2005)
  6. The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)2 Mungall A.J.... Beck S. (2003)
  7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
  8. Human immunodeficiency virus type 1 Tat protein impairs selenoglutathione peroxidase expression and activity by a mechanism independent of cellular selenium uptake: consequences on cellular resistance to UV-A radiation. (PubMed id 11368344)1 Richard M.J....Favier A. (2001)
  9. Molecular mechanism of decreased glutathione content in human immunodeficiency virus type 1 Tat-transgenic mice. (PubMed id 10652368)1 Choi J....Forman H.J. (2000)
  10. Glutathione depletion associated with the HIV-1 TAT protein mediates the extracellular appearance of acidic fibroblast growth factor. (PubMed id 9501919)1 Opalenik S.R....Thompson J.A. (1998)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 257202 HGNC: 4558 AceView: GPX6 Ensembl:ENSG00000198704 euGenes: HUgn257202
ECgene: GPX6 Kegg: 257202 H-InvDB: GPX6

(According to HUGE)
About This Section
  --

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
NIEHS-SNPshttp://egp.gs.washington.edu/data/gpx6/

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for GPX6 gene:
Search GeneIP for patents involving GPX6

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

 Millipore Custom Antibody & Bulk Services
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 "Your Favorite Gene" Pathways and Molecular Interaction Networks Browse Nano Scale siRNA at Sigma-Aldrich
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 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX6
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 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays
 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX6
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 ELISAs and CLIAs for GPX6
       

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