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GPX8 Gene

protein-coding   GIFtS: 36

GC05P051429
glutathione peroxidase 8 (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
glutathione peroxidase 8 (putative)1 2     GSHPx-82 3
EPLA8471 2     OTTHUMP000002223862
UNQ8471 2     probable glutathione peroxidase 82
GPx-82 3     EC 1.11.1.93

External Ids:    HGNC: 331001   Entrez Gene: 4938692   Ensembl: ENSG000001642947   UniProtKB: Q8TED13   

Export aliases for GPX8 gene to outside databases

Previous GC identifer: GC05P054493


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for GPX8
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for GPX8

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q11.2   Ensembl cytogenetic band:  5q11.2   HGNC cytogenetic band: 5q11.2

GPX8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GPX8 gene location

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P051429:  view genomic region     (about GC identifiers)

Start:
51,429,158 bp from pter
End:
51,436,302 bp from pter
Size:
7,145 bases
Orientation:
plus strand

1 alternative location:
Chr5+ 54,455,946-54,462,899     
RefSeq DNA sequence:
NC_000005.9  NT_006713.15  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: GPX8_HUMAN, Q8TED1 (See protein sequence)
Recommended Name: Probable glutathione peroxidase 8  
Size: 209 amino acids; 23881 Da
Subcellular location: Membrane; Single-pass membrane protein (Potential)
PDB structures from and Proteopedia :
3CYN (3D)    

Explore the universe of human proteins at neXtProt for GPX8: NX_Q8TED1 

REFSEQ proteins: NP_001008398.2  

ENSEMBL proteins: 
ENSP00000423822 ENSP00000427466 ENSP00000296734 ENSP00000430956 


Human Recombinant Proteins 
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OriGene Protein Over-expression Lysate: GPX8
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Novus Biologicals Protein for GPX8
Novus Biologicals Lysates for GPX8
Browse Sino Biological Recombinant Proteins
Browse ProSpec Recombinant Proteins

2 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

GPX8 for ontologies           About GeneDecksing



Antibodies for GPX8: 
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Browse antibodies at Epitomics

Assays for GPX8: 
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Browse R&D Systems for biochemical assays
GenScript Custom Assay Services for GPX8 
Browse Enzo Life Sciences for kits & assays
Browse ELISAs and CLIAs at Uscn 


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

GPX8 for domains           About GeneDecksing

4 InterPro domains/families:
 IPR013376 Glut_perox_Gpx7
 IPR012336 Thioredoxin-like_fold
 IPR012335 Thioredoxin_fold
 IPR000889 Glutathione_peroxidase

Graphical View of Domain Structure for InterPro Entry Q8TED1

ProtoNet protein and cluster: Q8TED1

1 Blocks protein family: IPB000889 Glutathione peroxidase

UniProtKB/Swiss-Prot: GPX8_HUMAN, Q8TED1
Similarity: Belongs to the glutathione peroxidase family


(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: GPX8_HUMAN, Q8TED1
Catalytic activity: 2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Enzyme Number (IUBMB): EC 1.11.1.9 

Inhib.
RNA:
    
Browse for Gene Knock-down Tools from Millipore
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OriGene 29mer shRNA kits in GFP-retroviral vector: GPX8
OriGene shRNA RFP: GPX8
OriGene basic RS shRNA: GPX8
OriGene siRNA: GPX8
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX8 
miRNA:Sigma-Aldrich microRNA Mimics for GPX8
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX8 
3 SABiosciences Assays for microRNAs that regulate GPX8:
hsa-miR-607 hsa-miR-582-5p hsa-miR-601

Gene
Editing:
Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: GPX8
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPX8
OriGene untagged cDNA clones in CMV expression vector (see all 2): GPX8
OriGene 3'-UTR clone: GPX8 
Browse MicroRNA Expression Plasmids 
GenScript Custom cDNA clone Services for GPX8 
Browse Sino Biological Human cDNA Clones

Cell
Lines:
  
GenScript Custom overexpressing Cell Line Services for GPX8 

2 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004602 glutathione peroxidase activity IEA--
GO:0016491 oxidoreductase activity IEA--
About this table

GPX8 for ontologies           About GeneDecksing


(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section


GPX8 for pathways           About GeneDecksing

2 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  GPX8
    Glutathione Metabolism
Arachidonic Acid Metabolism

    SABiosciences Pathway-Focused PCR Array including GPX8: PAHS-094A 

    Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for GPX8
    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for GPX8

2 Gene Ontology (GO) biological process terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006979 response to oxidative stress IEA--
GO:0055114 oxidation-reduction process IEA--
About this table

GPX8 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for GPX8


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib.
RNA:
     
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich siRNA Panels and siRNA for GPX8
Sigma-Aldrich shRNA for GPX8
OriGene 29mer shRNA kits in GFP-retroviral vector: GPX8
OriGene shRNA RFP: GPX8
OriGene basic RS shRNA: GPX8
OriGene siRNA: GPX8
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX8 
miRNA: Sigma-Aldrich microRNA Mimics for GPX8
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX8 
3 SABiosciences Assays for microRNAs that regulate GPX8:
hsa-miR-607 hsa-miR-582-5p hsa-miR-601
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: GPX8
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: GPX8
OriGene untagged cDNA clones in CMV expression vector (see all 2): GPX8
OriGene 3'-UTR Clone: GPX8 
Browse OriGene MicroRNA Expression Plasmids 
GenScript Custom cDNA clone Services for GPX8 
Primers: OriGene genome-wide validated SYBR primer pairs: GPX8
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for GPX8: PPH57927A

REFSEQ mRNAs for GPX8 gene: 

NM_001008397.2  

Additional cDNA sequence: 

AF086192.1 AK022110.1 AK074216.1 AK298545.1 AK307365.1 AY358715.1 BC029424.1 BX537894.1 
BX647770.1 CR620005.1 CR623605.1 

8 DOTS entries:

DT.212649  DT.92423608  DT.92423609  DT.100801024  DT.95266794  DT.110213  DT.97759851  DT.100024994 

Unigene Cluster for GPX8:

Glutathione peroxidase 8 (putative)
Hs.289044  [show with all ESTs]
Unigene Representative Sequence: NM_001008397


GeneLoc Exon Structure

9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000511617 ENST00000505096 ENST00000503787 ENST00000515370 ENST00000506123(uc003jps.2 uc003jpt.2)
ENST00000514317 ENST00000296734(uc003jpq.2 uc003jpr.2) ENST00000521115
ENST00000502733

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

GPX8 expression in normal and diseased human tissues

1  / 2

5 probe-sets matching GPX8 gene

Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
52123_at2 U95-B 1 0.69 1.00 1.00 1.00 -- -- -- -- --
227628_at2 U133-B 1 0.64 1.00 -- -- -- -- -- -- --
228141_at2 U133-B 1 0.46 1.00 -- -- -- -- -- -- --
227628_at2 U133Plus2 1 0.64 1.00 -- -- -- -- -- -- --
228141_at2 U133Plus2 1 0.46 1.00 -- -- -- -- -- -- --
About this table

GPX8 for expression           About GeneDecksing

Data from Genenote  (Publications) and GNF BioGPS
    About these images
GPX8 gene expression
GPX8 gene electronic northern expression
GPX8 gene sage expression
About these images

CGAP SAGE TAG: AAAGAGTATT

SOURCE GeneReport for Unigene cluster: Hs.289044
Primers: OriGene genome-wide validated SYBR primer pairs: GPX8
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for GPX8: PPH57927A
    SABiosciences Expression via Pathway-Focused PCR Array including GPX8: PAHS-094A 


(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for GPX8 gene from 5/8 species (see all 8)
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
LOC4780591   -- similar to F26E4.12 91.55(n)
89(a)
478059  XM_535237.2  XP_535237.1 
chimpanzee
(Pan troglodytes)
GPX81   -- glutathione peroxidase 8 (putative) 99.36(n)
99.52(a)
461872  XM_001147832.1  XP_001147832.1 
cow
(Bos taurus)
GPX81   -- glutathione peroxidase 8 (putative) 91.71(n)
89.47(a)
511575  NM_001046088.1  NP_001039553.1 
rat
(Rattus norvegicus)
Gpx81   -- glutathione peroxidase 8 83.89(n)
83.73(a)
294744  XM_215486.4  XP_215486.2 
mouse
(Mus musculus)
Gpx81 , 5 13 (63.93 cM)5
glutathione peroxidase 8 (putative)1, 5 82.78(n)1
82.78(a)1
695901  NM_027127.21  NP_081403.11 
 AI8387135  AK0093785  (see all 7)
About this table        Species with no ortholog for GPX8

ENSEMBL Gene Tree for GPX8 (if available)

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
Paralogs for GPX8 gene
GPX62  GPX52  GPX22  GPX42  GPX72  GPX12  GPX32  

GPX8 for paralogs           About GeneDecksing



(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
QIAGEN SeqTarget long-range PCR primers for resequencing GPX8 

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section
  --

(Possibly Related Articles in Doctor's Guide)
About This Section
  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

7 PubMed articles for GPX8 gene integrated from 7 sources:
(articles sorted by number of sources associating them with GPX8)
  1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
  2. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
  3. Evolutionary and structural insights into the multifaceted glutathione peroxidase (Gpx) superfamily. (PubMed id 18498225)1 Toppo S....Tosatto S.C. (2008)
  4. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
  5. The DNA sequence and comparative analysis of human chromosome 5. (PubMed id 15372022)2 Schmutz J.... Rubin E.M. (2004)
  6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
  7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 493869 HGNC: 33100 Ensembl:ENSG00000164294 euGenes: HUgn493869 ECgene: GPX8
H-InvDB: GPX8

(According to HUGE)
About This Section
  --

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
  --

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for GPX8 gene:
Search GeneIP for patents involving GPX8

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

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 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GPX8
 SeqTarget long-range PCR primers for resequencing GPX8
 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays
 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GPX8
 GenScript Custom Purified and Recombinant Proteins Services for GPX8 GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for GPX8
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