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GRB2 Gene

protein-coding   GIFtS: 67

GC17M068724
growth factor receptor-bound protein 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
growth factor receptor-bound protein 21 2     MST0842
NCKAP21 2     growth factor receptor-bound protein 32
Protein Ash2 3     OTTHUMP000001660972
Adapter protein GRB22 3     EGFRBP-GRB22
SH2/SH3 adapter GRB22 3     MSTP0842
ASH2 3     abundant SRC homology2
epidermal growth factor receptor-binding protein GRB22     HT0272
OTTHUMP000001660982     Grb3-32

External Ids:    HGNC: 45661   Entrez Gene: 28852   Ensembl: ENSG000001778857   UniProtKB: P629933   

Export aliases for GRB2 gene to outside databases

Previous GC identifers: GC17M073313 GC17M076374 GC17M073779 GC17M073911 GC17M070825


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for GRB2:
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3
domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain
binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the
signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found
for this gene. (provided by RefSeq)

UniProtKB/Swiss-Prot: GRB2_HUMAN, P62993
Function: Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras
signaling pathway
Function: Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits
EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2
and by suppressing proliferative signals, may trigger active programmed cell death

Gene Wiki entry for GRB2 (Grb2)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the GRB2 gene promoter:
         p53   c-Myc   Max   Max1   STAT1beta   STAT1alpha   STAT1   Elk-1   GATA-2   Sp1   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for GRB2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q24-q25   Ensembl cytogenetic band:  17q25.1   HGNC cytogenetic band: 17q24-q25

GRB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
GRB2 gene location

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M068724:  view genomic region     (about GC identifiers)

Start:
68,724,752 bp from pter
End:
68,824,731 bp from pter
Size:
99,980 bases
Orientation:
minus strand

1 alternative location:
Chr17- 73,314,157-73,401,790     
RefSeq DNA sequence:
NC_000017.10  NT_010783.15  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: GRB2_HUMAN, P62993 (See protein sequence)
Recommended Name: Growth factor receptor-bound protein 2  
Size: 217 amino acids; 25206 Da
Subunit: Associates with activated Tyr-phosphorylated EGF receptors and PDGF receptors via its SH2 domain. Also
associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK; probably via the
concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading
to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated
TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT,
LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with NISCH, PTPNS1, REPS2 and the syntrophin SNTA1. Interacts
with REPS1 and PIK3C2B via its SH3 domains (By similarity). Interacts with HCV NS5A via its SH3 domains. Interacts
with CBL and CBLB. Interacts with JUB and CLNK (By similarity). Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2.
Forms a complex with MUC1 and SOS1, through interaction of the SH3 domains with SOS1 and the SH2 domain with
phosphorylated MUC1. Interacts with PRNP and THEMIS (By similarity). Interacts with RALGPS1 and with HCST. Interacts
(via SH3 domain) with HEV ORF3 protein. Interacts with GAPT and PTPRE. Interacts (via SH2 domain) with KIF26A.
Interacts (via SH3 2) with GAB2. Interacts with ADAM15. Interacts with THEMIS2 (By similarity)
Subcellular location: Golgi apparatus (By similarity)
PDB structures from and Proteopedia :
1AZE (3D)    1BM2 (3D)    1BMB (3D)    1CJ1 (3D)    1FHS (3D)    1FYR (3D)    1GCQ (3D)    1GFC (3D)    
1GFD (3D)    1GHU (3D)    1GRI (3D)    1IO6 (3D)    1JYQ (3D)    1JYR (3D)    1JYU (3D)    1QG1 (3D)    
1TZE (3D)    1X0N (3D)    1ZFP (3D)    2AOA (3D)    2AOB (3D)    2H46 (3D)    2H5K (3D)    2HUW (3D)    
2VVK (3D)    2VWF (3D)    2W0Z (3D)    3C7I (3D)    3IMD (3D)    3IMJ (3D)    3IN7 (3D)    3IN8 (3D)    
3KFJ (3D)    
Secondary accessions: P29354 Q14450 Q63057 Q63059
Alternative splicing: 2 isoforms:  P62993-1, P29354-1   P62993-2, P29354-2   (Additional isoforms seem to exist)

Explore the universe of human proteins at neXtProt for GRB2: NX_P62993 

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_002077.1  NP_987102.1  


    ENSEMBL proteins: 
    ENSP00000339007 ENSP00000376345 ENSP00000376346 ENSP00000376347 ENSP00000317360 


    Human Recombinant Proteins 
    Millipore Purified and/or Recombinant GRB2 Protein
    Sigma-Aldrich Proteins for GRB2
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein: GRB2
    OriGene Protein Over-expression Lysate: GRB2
    GenScript Custom Purified and Recombinant Proteins Services for GRB2 
    Novus Biologicals Proteins for GRB2
    Novus Biologicals Lysate for GRB2
    Browse Sino Biological Recombinant Proteins
    ProSpec Recombinant Protein for GRB2

    5/6 Gene Ontology (GO) cellular component terms (GO ID links to tree view) (see all 6):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    GO:0005794 Golgi apparatus IEA--
    GO:0005829 cytosol TAS--
    GO:0012506 vesicle membrane IEA--
    GO:0016020 membrane IEA--
    About this table

    GRB2 for ontologies           About GeneDecksing



    Antibodies for GRB2: 
    Millipore Mono- and Polyclonal Antibodies for the study of GRB2
    Sigma-Aldrich Antibody Arrays and Antibodies for GRB2
    R&D Systems Antibodies for GRB2
    Cell Signaling Technology (CST) Antibodies for GRB2 
    OriGene Antibodies (see all 4): GRB2
    GenScript Custom Superior Antibodies Services for GRB2 
    Novus Biologicals Antibodies for GRB2
    Epitomics antibodies for GRB2

    Assays for GRB2: 
    Browse Kits and Assays available from Millipore
    Browse ELISAs at Sigma-Aldrich
    OriGene Custom Immunoassay Development 
    Browse OriGene Fluorogenic Cell Assay Kits 
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for GRB2 
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs and CLIAs at Uscn 


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    GRB2 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR000108 p67phox
     IPR001452 SH3_domain
     IPR000980 SH2

    Graphical View of Domain Structure for InterPro Entry P62993

    ProtoNet protein and cluster: P62993

    UniProtKB/Swiss-Prot: GRB2_HUMAN, P62993
    Domain: The SH3 domains mediate interaction with RALGPS1 and SHB
    Similarity: Belongs to the GRB2/sem-5/DRK family
    Similarity: Contains 1 SH2 domain
    Similarity: Contains 2 SH3 domains


    (According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
    About This Section

    UniProtKB/Swiss-Prot: GRB2_HUMAN, P62993
    Function: Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras
    signaling pathway
    Function: Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits
    EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2
    and by suppressing proliferative signals, may trigger active programmed cell death

    Inhib.
    RNA:
        
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for GRB2
    Sigma-Aldrich shRNA Panels and shRNA for GRB2
    OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): GRB2
    OriGene shRNA RFP (see all 2): GRB2
    OriGene basic RS shRNA (see all 2): GRB2
    OriGene siRNA (see all 2): GRB2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GRB2 
    miRNA:Sigma-Aldrich microRNA Mimics for GRB2
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GRB2 
    8/16 SABiosciences Assays for microRNAs that regulate GRB2 (see all 16):
    hsa-miR-362-3p hsa-miR-1288 hsa-miR-513a-5p hsa-miR-200a hsa-miR-105 hsa-miR-141 hsa-miR-124 hsa-miR-506

    Gene
    Editing:
    Browse CompoZr Knockout ZFN at Sigma-Aldrich 

    Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
    Browse iPSC Reprogramming Factors at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene untagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene 3'-UTR clone (see all 2): GRB2 
    Browse MicroRNA Expression Plasmids 
    GenScript Custom cDNA clone Services for GRB2 
    Browse Sino Biological Human cDNA Clones

    Cell
    Lines:
      
    GenScript Custom overexpressing Cell Line Services for GRB2 

    Genatlas biochemistry entry for GRB2:
    growth factor receptor bound protein 2,major CBL associated protein,homolog to Caenorhabditis elegans Sem5 gene and
    Drosophila Drk,STAR playing a key role in insulin signal transduction through connecting with IRS1 and IGFR5,and in
    mitogenic RAS signalic through interaction with SHC1

    5/7 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 7):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001784 phosphotyrosine binding IEA--
    GO:0005070 SH3/SH2 adaptor activity TAS8253073
    GO:0005154 epidermal growth factor receptor binding IPI12577067
    GO:0005515 protein binding IPI7523381
    GO:0019904 protein domain specific binding IEA--
    About this table

    GRB2 for ontologies           About GeneDecksing

    Animal Models: 15 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Grb2):

    behavior/neurologicalcardiovascular systemcraniofacialdigestive/alimentaryembryogenesis
    growth/sizehearing/vestibular/earhematopoietic systemhomeostasis/metabolismimmune system
    lethality-prenatal/perinatalnervous systemnormalskeletontumorigenesis

    GRB2 for phenotypes           About GeneDecksing


    (Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
    About This Section


    GRB2 for pathways           About GeneDecksing

    5/80 Millipore Pathways for GRB2 (see all 80)
        Chemotaxis CXCR4 signaling pathway
    Immune response CD16 signaling in NK cells
    Cytoskeleton remodeling FAK signaling
    Cell adhesion Integrin-mediated cell adhesion and migration
    G-protein signaling G-Protein beta/gamma signaling cascades

    5/86 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  GRB2 (see all 86)
        TGF-β Signaling
    Regulation of eIF4 and p70S6K Signaling
    Acute Myeloid Leukemia Signaling
    Glucocorticoid Receptor Signaling
    Cardiac Hypertrophy Signaling

    5/6 Cell Signaling Technology (CST) Pathways for GRB2 (see all 6)
        Jak/Stat Signaling: IL-6 Receptor Family
    Insulin Receptor Signaling
    T Cell Receptor Signaling
    B Cell Receptor Signaling
    MAPK/Erk in Growth and Differentiation

    5/23 Kegg Pathways  (Kegg details for GRB2) (see all 23):
        hsa04010 MAPK signaling pathway
    hsa04012 ErbB signaling pathway
    hsa04062 Chemokine signaling pathway
    hsa04320 Dorso-ventral axis formation
    hsa04510 Focal adhesion

        SABiosciences Pathway-Focused PCR Arrays including GRB2 (see all 6): PAHS-030A PAHS-040A  

        Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for GRB2
        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for GRB2

    5/469 Interacting proteins for GRB2 (P629931, 2 ENSP000003390073) via UniProtKB, MINT, and/or STRING (see all 469)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EGFRP005331, 2, ENSP000002754933EBI-401755,EBI-297353 MINT-8029342 MINT-8029357 MINT-74541 MINT-61494 MINT-8029326 MINT-58642 MINT-8029310 MINT-1793777 MINT-61502 MINT-8029261 MINT-61540 MINT-8038388 MINT-6169986 MINT-8038373 MINT-61498 MINT-61500 MINT-58641 MINT-61496 MINT-8029372 MINT-58640 MINT-61497 MINT-8029231 STRING: ENSP00000275493
    SOS1Q078891, 2, ENSP000002638793EBI-401755,EBI-297487 MINT-1782504 MINT-7217748 MINT-7135453 MINT-6173175 MINT-6170102 MINT-1782591 MINT-7217714 MINT-7217731 MINT-7217692 MINT-7253710 MINT-7217665 MINT-7135473 MINT-6170081 MINT-61539 MINT-6170118 MINT-65980 STRING: ENSP00000263879
    MAP4K1Q929181, 2, ENSP000002214093EBI-401755,EBI-881 MINT-7212988 MINT-7213002 MINT-7213078 MINT-7213120 MINT-7212968 MINT-7213066 MINT-7253194 STRING: ENSP00000221409
    PTPN11Q061241, 2, ENSP000003409443EBI-401755,EBI-297779 MINT-7300447 MINT-61552 STRING: ENSP00000340944
    DNM1Q051931, 2, ENSP000003620143EBI-401755,EBI-713135 MINT-65952 MINT-65951 STRING: ENSP00000362014
    About this table

    5/24 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 24):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007173 epidermal growth factor receptor signaling pathway TAS1322798
    GO:0007265 Ras protein signal transduction TAS8253073
    GO:0007267 cell-cell signaling TAS8253073
    GO:0007411 axon guidance TAS--
    GO:0007568 aging IEA--
    About this table

    GRB2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    GRB2 for compounds           About GeneDecksing

    Browse Small Molecules at Sigma-Aldrich
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for GRB2
    10/52 Novoseek chemical compound relationships for GRB2 gene (see all 52)
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 89.5 626 8910422 (5), 8388384 (5), 8491186 (4), 11726515 (4) (see all 99)
    phosphotyrosine 84.4 50 10356320 (2), 8391166 (1), 9183666 (1), 11689444 (1) (see all 34)
    phosphatidylinositol 80.1 91 7961682 (3), 7791764 (3), 7692230 (2), 9565634 (2) (see all 72)
    pp36 79.1 5 9541596 (2), 9529333 (2), 7544353 (1)
    ag 1478 55.7 1 9722539 (1)
    phosphoinositide 49 17 10330169 (1), 12220227 (1), 19438505 (1), 15579470 (1) (see all 13)
    letrozole 45.7 8 15958587 (2), 15967659 (1), 17113978 (1), 19250202 (1) (see all 6)
    wortmannin 45.6 1 16267404 (1)
    phosphatidylinositol-3,4,5-trisphosphate 45.4 2 17923698 (1)
    phenylalanine 44.9 9 11002419 (1), 16407827 (1), 7559570 (1), 8959326 (1) (see all 9)
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Millipore,
    siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
    Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
    About This Section

    Inhib.
    RNA:
         
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for GRB2
    Sigma-Aldrich shRNA Panels and shRNA for GRB2
    OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): GRB2
    OriGene shRNA RFP (see all 2): GRB2
    OriGene basic RS shRNA (see all 2): GRB2
    OriGene siRNA (see all 2): GRB2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of GRB2 
    miRNA: Sigma-Aldrich microRNA Mimics for GRB2
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of GRB2 
    8/16 SABiosciences Assays for microRNAs that regulate GRB2 (see all 16):
    hsa-miR-362-3p hsa-miR-1288 hsa-miR-513a-5p hsa-miR-200a hsa-miR-105 hsa-miR-141 hsa-miR-124 hsa-miR-506
    Clones: Browse FLAG tag genes at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene untagged cDNA clones in CMV expression vector (see all 2): GRB2
    OriGene 3'-UTR Clone (see all 2): GRB2 
    Browse OriGene MicroRNA Expression Plasmids 
    GenScript Custom cDNA clone Services for GRB2 
    Primers: OriGene genome-wide validated SYBR primer pairs: GRB2
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for GRB2: PPH00714A

    REFSEQ mRNAs for GRB2 gene (2 alternative transcripts): 

    NM_002086.4  NM_203506.2  

    Additional cDNA sequence: 

    AF171699.1 AF246238.1 AF302079.1 AF498925.1 AK024539.1 AK091010.1 AK315565.1 BC000631.2 
    BC019082.2 BC033644.1 BC039827.1 BC043007.1 BC127778.1 CR450363.1 CR541942.1 L29511.1 
    M96995.1 X62852.1 

    24/27 DOTS entries (see all 27):

    DT.91786336  DT.101985106  DT.455862  DT.100833847  DT.95197797  DT.97788620  DT.100827631  DT.91768193 
    DT.100827630  DT.121008073  DT.121008099  DT.97776617  DT.100039623  DT.92454820  DT.121008170  DT.95197812 
    DT.121008094  DT.100827635  DT.100827636  DT.121008122  DT.65285546  DT.100827634  DT.121008141  DT.91710775 

    24/631 AceView cDNA sequences (see all 631):

    AI364084 CA435956 BU730769 BM695652 BQ879498 AF498925 BP339140 AI446232 
    AU142798 H13810 BU521520 AW302611 F02165 BU195750 AI355116 BX353450 
    BQ012409 BE221139 BF446179 AI073688 BU687777 AI682636 AA373713 CA306094 

    highest scoring ESTs for GRB2:

    AA313802 AI439688 AI815684 AL134982 AU142798 AW161184 AW674664 BC000631 BE387478 BE396668 

    Unigene Cluster for GRB2:

    Growth factor receptor-bound protein 2
    Hs.444356  [show with all ESTs]
    Unigene Representative Sequence: NM_002086


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for GRB2 (see all 7)

    ExUns: 1 ^ 2 ^ 3a · 3b · 3c · 3d · 3e ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d
    SP1:        -     -     -                 -     -                                                   
    SP2:                                      -     -                                                   
    SP3:                                      -     -                       -                           
    SP4:                                      -     -                 -                                 
    SP5:        -     -     -                 -                                                         

    About this scheme

    ECgene alternative splicing isoforms for GRB2
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000316804(uc002jnx.3) ENST00000392562 ENST00000392563 ENST00000392564
    ENST00000462266 ENST00000316615(uc002jny.3)

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Primers from OriGene and/or SABiosciences )
    About This Section

    GRB2 expression in normal and diseased human tissues

    1  / 2  / 3

    7 probe-sets matching GRB2 gene

    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    46217_at2, 3 U95-B 1 1.00 1.00 0.85 1.35 AI439688 0.80 1.00 0.91 1
    1565_s_at2, 3 U95-A 1 0.81 1.00 0.48 0.82 M96995 0.80 1.00 0.91 1
    33855_at2, 3 U95-A 1 0.50 1.00 0.66 0.84 M96995 0.80 1.00 0.91 1
    223049_at2, 3 U133-B 1 1.00 1.00 -- -- AF246238 0.60 1.00 0.82 1
    215075_s_at2, 3 U133-A 1 0.46 1.00 -- -- L29511 0.60 1.00 0.82 1
    223049_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    215075_s_at2 U133Plus2 1 0.46 1.00 -- -- -- -- -- -- --
    About this table

    GRB2 for expression           About GeneDecksing

    Data from Genenote  (Publications) and GNF BioGPS
        About these images
    GRB2 gene expression
    GRB2 gene electronic northern expression
    GRB2 gene sage expression
    About these images

    CGAP SAGE TAG: AAGCCTTGCT

    SOURCE GeneReport for Unigene cluster: Hs.444356

    Expression variation in blood from EXPOLDB for GRB2

    Primers: OriGene genome-wide validated SYBR primer pairs: GRB2
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for GRB2: PPH00714A
        SABiosciences Expression via Pathway-Focused PCR Arrays including GRB2 (see all 6): PAHS-030A PAHS-040A  


    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section

    Orthologs for GRB2 gene from 5/13 species (see all 13)
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    GRB21   -- growth factor receptor-bound protein 2 94.78(n)
    100(a)
    483312  XM_540431.2  XP_540431.2 
    chimpanzee
    (Pan troglodytes)
    GRB21   -- growth factor receptor-bound protein 2 100(n)
    100(a)
    737245  XM_001141640.1  XP_001141640.1 
    cow
    (Bos taurus)
    GRB21   -- growth factor receptor-bound protein 2 93.86(n)
    100(a)
    535298  NM_001034630.1  NP_001029802.1 
    rat
    (Rattus norvegicus)
    Grb21   -- growth factor receptor bound protein 2 92.01(n)
    100(a)
    81504  NM_030846.2  NP_110473.2 
    mouse
    (Mus musculus)
    Grb21 , 5 11 (80.91 cM)5
    growth factor receptor bound protein 21, 5 92.17(n)1
    99.54(a)1
    147841  NM_008163.31  NP_032189.11 
     AA4081645  AI3237185  (see all 26)
    About this table        Species with no ortholog for GRB2

    ENSEMBL Gene Tree for GRB2 (if available)

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section
    Paralogs for GRB2 gene
    GRAP2  GRAPL2  NCK12  NCK22  GRAP22  

    GRB2 for paralogs           About GeneDecksing



    (SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
    About This Section

    10/1015 NCBI SNPs in GRB2 are shown (see all 1015)
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ----------
    rs72191,2
    C,F,A,H,73315368(-) TAAAGA/GCAGTC 2 -- ut31 ese314Minor allele frequency- G:0.33MN NS EA NA 856
    rs7559681,2
    --73365277(-) TTTATA/GTACCA 2 -- int10--------
    rs8927681,2
    C,A,H,73366602(-) AGGGAC/TGCGTT 2 -- int110Minor allele frequency- T:0.05NA PA WA 266
    rs9592601,2
    C,F,O,H,73369422(-) TTGACA/GTTCTG 2 -- int139Minor allele frequency- G:0.36NA EA NS 3794
    rs10436171,2
    --73314441(-) CTCTTC/TTGGGC 2 -- ut31 ese31Minor allele frequency- T:0.00MN 184
    rs10438241,2
    --73389838(-) GGCATT/CGTGTG 2 -- ut51 ese31Minor allele frequency- C:0.00MN 184
    rs10760941,2
    C,F,O,A,73344711(+) GGAAAC/TTGCTG 2 -- int128Minor allele frequency- T:0.35NA EA NS 2095
    rs11083551,2
    C,F,O,H,73383913(-) CTAAGA/TTGTTG 2 -- int115Minor allele frequency- T:0.28NS EA NA 730
    rs11090331,2
    C,F,H,73333569(+) GCTGCG/ACCTAC 2 -- int117Minor allele frequency- A:0.31NA EA NS 2322
    rs13692991,2
    C,F,A,H,73347325(+) CCCTCC/TCAAAA 2 -- int1 trp325Minor allele frequency- T:0.32NS EA NA 4214
    About this table

    HapMap Linkage Disequilibrium images for GRB2 (up to first 250kb)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for GRB2
         2 CNVs: 5030 5881

    QIAGEN SeqTarget long-range PCR primers for resequencing GRB2 

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    GRB2 for disorders           About GeneDecksing

    OMIM: 108355

    10/31 Novoseek disease relationships for GRB2 gene (see all 31)

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    myeloid leukemia chronic 37.9 1 15143164 (1)
    wiskott-aldrich syndrome 37.3 3 9307968 (2), 15579908 (1)
    breast cancer 36.4 33 11175341 (5), 10022814 (5), 8058337 (3), 9390033 (2) (see all 16)
    neoplastic transformation 32.3 2 8084588 (1), 8662827 (1)
    leukemia 21.4 9 9096689 (1), 15143164 (1), 15050920 (1), 10887132 (1) (see all 8)
    leukemogenesis 20.2 2 15143164 (2)
    tumors 17.9 35 11606575 (2), 16309242 (2), 15958587 (2), 12850474 (1) (see all 27)
    cancer 16.7 20 18620523 (2), 9009162 (1), 18216394 (1), 12878216 (1) (see all 14)
    myeloproliferative disorders 6.3 1 10887132 (1)
    melanoma 5.33 1 10091608 (1)
    About this table

    Human Genome Epidemiology (HuGE) Navigator: GRB2 (10 documents)
    Tumor Gene Database (TGDB): GRB2

    Export disorders and mutations for GRB2 gene to outside databases

    (Possibly Related Articles in Doctor's Guide)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
    About This Section

    10/1099 PubMed articles for GRB2 gene, integrated from 7 sources (see all 1099):
    (articles sorted by number of sources associating them with GRB2)
    1. The SH2/SH3 domain-containing protein GRB2 interacts with tyrosine- phosphorylated IRS1 and Shc: implications for insulin control of ras signalling. (PubMed id 8491186)1, 2, 7 Skolnik E.Y....Schlessinger J. (1993)
    2. Insulin stimulates association of insulin receptor substrate-1 with the protein abundant Src homology/growth factor receptor-bound protein 2. (PubMed id 8388384)1, 2, 7 Tobe K.... Kadowaki T. (1993)
    3. Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2. (PubMed id 8723348)1, 2, 7 Kavanaugh W.M....Williams L.T. (1996)
    4. Crystal structures of the SH2 domain of Grb2: highlight on the binding of a new high-affinity inhibitor. (PubMed id 11827484)1, 2, 7 Nioche P.... Ducruix A. (2002)
    5. Recruitment of the class II phosphoinositide 3-kinase C2beta to the epidermal growth factor receptor: role of Grb2. (PubMed id 11533253)1, 2, 7 Wheeler M. and Domin J. (2001)
    6. Solution structure and ligand-binding site of the carboxy-terminal SH3 domain of GRB2. (PubMed id 7881903)1, 2, 7 Kohda D.... Inagaki F. (1994)
    7. Distinct binding modes of two epitopes in Gab2 that interact with the SH3C domain of Grb2. (PubMed id 19523899)1, 2, 7 Harkiolaki M.... Feller S.M. (2009)
    8. Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin. (PubMed id 10942756)1, 2, 7 Asazuma N.... Watson S.P. (2000)
    9. Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation. (PubMed id 10022120)1, 2, 7 Elly C....Liu Y.-C. (1999)
    10. Structural and conformational requirements for high-affinity binding to the SH2 domain of Grb2(1). (PubMed id 10090780)1, 2, 7 Ettmayer P.... Bair K.W. (1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
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    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 2885 HGNC: 4566 AceView: GRB2 Ensembl:ENSG00000177885 euGenes: HUgn2885
    ECgene: GRB2 Kegg: 2885 H-InvDB: GRB2

    (According to HUGE)
    About This Section
      --

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    ATLAS Chromosomes in Cancer entry for GRB2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from XenneX, Inc.)
    About This Section
    Patent Information for GRB2 gene:
    Search GeneIP for patents involving GRB2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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