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MT1B Gene

protein-coding GIFtS: 44

GC16P042555

metallothionein 1B
(Previous name: metallothionein 1Q )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: MT1, MT1Q)

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases & Descriptions
metallothionein 1B¹ ²		MTP²
metallothionein 1Q¹ ²		metallothionein-1B²
MT-1B² ³		metallothionein 1B (functional)²
MT-IB² ³		metallothionein-IB²
MT1Q² ³		Metallothionein-IB³
MT1²

External Ids:	HGNC: 7394¹	Entrez Gene: 4490²	Ensembl: ENSG00000169688⁷	UniProtKB: P07438³

Export aliases for MT1B gene to outside databases

Previous GC identifers: GC16P047069 GC16M056736 GC16P056420 GC16P056443 GC16P056448 GC16P055231 GC16P055243 GC16P056685

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: MT1B_HUMAN, P07438

Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are

transcriptionally regulated by both heavy metals and glucocorticoids

Gene Wiki entry for MT1B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the MT1B gene promoter:
         Sp1   HSF1 (long)   NF-1   deltaCREB   CREB   p300   TBP   USF1   POU2F1   c-Myb
         Other transcription factors

Search SABiosciences Chromatin IP Primers for MT1B

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q13 Ensembl cytogenetic band: 16q12.2 HGNC cytogenetic band: 16q13

MT1B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
MT1B gene location

GeneLoc gene densities for chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16P042555: view genomic region (about GC identifiers)

Start:	42,555,257 bp from pter
End:	42,556,561 bp from pter
Size:	1,305 bases
Orientation:	plus strand

1 alternative location:

Chr16+ 56,685,811-56,687,116

RefSeq DNA sequence:

NC_000016.9 NT_010498.15

(According to ¹UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
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UniProtKB/Swiss-Prot: MT1B_HUMAN, P07438 (See protein sequence)

Recommended Name: Metallothionein-1B

Size: 61 amino acids; 6115 Da

Subunit: Monomer

Explore the universe of human proteins at neXtProt for MT1B: NX_P07438

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_005938.1

ENSEMBL proteins:

ENSP00000334998

Human Recombinant Proteins

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1 Gene Ontology (GO) cellular component term (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005737	cytoplasm	NAS	--

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MT1B for ontologies About GeneDecksing

Antibodies for MT1B:

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Assays for MT1B:

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	GenScript Custom Assay Services for MT1B
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for MT1B

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

MT1B for domains About GeneDecksing

4 InterPro domains/families:

IPR000006 Metallothionein_vert

IPR018064 Metallothionein_vert_metal_BS

IPR003019 Metallothionein_sfam_euk

IPR017854 Metallothionein_sfam

Graphical View of Domain Structure for InterPro Entry P07438

ProtoNet protein and cluster: P07438

UniProtKB/Swiss-Prot: MT1B_HUMAN, P07438

Domain: Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of

the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the

corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines

Similarity: Belongs to the metallothionein superfamily. Type 1 family

(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: MT1B_HUMAN, P07438

Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are

transcriptionally regulated by both heavy metals and glucocorticoids

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Browse Nano Scale siRNA at Sigma-Aldrich Sigma-Aldrich esiRNA and siRNA for MT1B Sigma-Aldrich shRNA for MT1B
		OriGene 29mer shRNA kits in GFP-retroviral vector: MT1B OriGene shRNA RFP: MT1B OriGene basic RS shRNA: MT1B OriGene siRNA: MT1B
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miRNA:		Browse microRNA Mimics at Sigma-Aldrich Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of MT1B
		Search SABiosciences for Assays for microRNAs that regulate MT1B

Gene
Editing:

Browse CompoZr Knockout ZFN at Sigma-Aldrich

Clones:		Browse Clones for the Expression of Recombinant Proteins Available from Millipore
		Browse iPSC Reprogramming Factors at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector: MT1B OriGene Myc/DDK tagged cDNA clones in CMV expression vector: MT1B OriGene untagged cDNA clones in CMV expression vector: MT1B Browse 3'-UTR reporter clones for miRNA target validation Browse MicroRNA Expression Plasmids
		GenScript Custom cDNA clone Services for MT1B
		Browse Sino Biological Human cDNA Clones

Cell
Lines:

GenScript Custom overexpressing Cell Line Services for MT1B

Genatlas biochemistry entry for MT1B:

metallothionein 1B cluster

4 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005507	copper ion binding	NAS	--
GO:0008270	zinc ion binding	NAS	--
GO:0046870	cadmium ion binding	NAS	--
GO:0046872	metal ion binding	IEA	--

About this table

MT1B for ontologies About GeneDecksing

(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

SABiosciences Custom Pathway-Focused PCR Arrays for MT1B

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for MT1B

1 Gene Ontology (GO) biological process term (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008150	biological_process	ND	--

About this table

MT1B for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

MT1B for compounds About GeneDecksing

Browse Small Molecules at Sigma-Aldrich

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for MT1B
1 Novoseek chemical compound relationship for MT1B gene

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
cadmium	57	1	15952643 (1)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Browse Nano Scale siRNA at Sigma-Aldrich Sigma-Aldrich esiRNA and siRNA for MT1B Sigma-Aldrich shRNA for MT1B
		OriGene 29mer shRNA kits in GFP-retroviral vector: MT1B OriGene shRNA RFP: MT1B OriGene basic RS shRNA: MT1B OriGene siRNA: MT1B
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of MT1B

miRNA:		Browse microRNA Mimics at Sigma-Aldrich Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of MT1B
		Search SABiosciences for Assays for microRNAs that regulate MT1B

Clones:		Browse FLAG tag genes at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector: MT1B OriGene Myc/DDK tagged cDNA clones in CMV expression vector: MT1B OriGene untagged cDNA clones in CMV expression vector: MT1B Browse 3'-UTR reporter clones for miRNA target validation Browse OriGene MicroRNA Expression Plasmids
		GenScript Custom cDNA clone Services for MT1B

Primers:		Browse OriGene genome-wide validated SYBR primer pairs Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for MT1B: PPH09040A

REFSEQ mRNAs for MT1B gene:

NM_005947.2

Additional cDNA sequence:

AF349000.2 AF479613.1 AY168638.1 BC069421.1 BC137478.1 BC137479.1

6 DOTS entries:

DT.120684974 DT.406271 DT.100008764 DT.101982092 DT.100001400 DT.120684801

21 AceView cDNA sequences:

H72723 BX118979 BC069421 BF973961 NM_176870 NM_005947 AY168638 AA370427

AI719425 BC028280 BU663337 BG217075 AF349000 R99207 H72722 BG183187

BG221550 BG192094 R99208 BG220590 BG195149

highest scoring ESTs for MT1B:

M13485 AF349000 AY168638 BC069421 BX118979 H72723 BU663337 H72722 NM_005947 R99207

Unigene Cluster for MT1B:

Metallothionein 1B

Hs.656629 [show with all ESTs]

Unigene Representative Sequence: BU663337

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation, and UCSC links where relevant:

ENST00000334346(uc002ejs.2)

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

MT1B expression in normal and diseased human tissues

¹ / ² / ³

2 probe-sets matching MT1B gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
73455_f_at²^{, 3}	U95-E	10	0.93	0.41	0.99	1.07	H72723	0.40	0.67	0.55	1
609_f_at²^{, 3}	U95-A	14	0.69	0.60	0.96	0.93	M13485	0.20	1.00	0.72	1

About this table

MT1B for expression About GeneDecksing

Data from Genenote (Publications) and GNF BioGPS
About these images

MT1B gene electronic northern expression

About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.656629

Expression variation in blood from EXPOLDB for MT1B

Primers:		Browse OriGene genome-wide validated SYBR primer pairs Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for MT1B: PPH09040A

SABiosciences Custom PCR Arrays for MT1B

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for MT1B gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	MT1B¹	--	metallothionein 1B	98.36(n) 98.36(a)	467981 XM_001139997.1 XP_001139997.1

About this table Species with no ortholog for MT1B

ENSEMBL Gene Tree for MT1B (if available)

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for MT1B gene

MT1E² MT1F² MT2A² MT1CP² MT4² MT1M² MT1A² MT3²

MT1H²

MT1B for paralogs About GeneDecksing

(SNPs according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

SNP ID	Valid	Chr 16 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample
Genomic Data				Transcription Related Data				Allele Frequencies
			--	--	--		--	--
rs964372^1,2	C,F,A,	56686030(+)	`TCTTGG/CCCAAG`	1	--	int¹		15	EA NA NS WA	1984
rs964374^1,2	C,	56686471(+)	`TTTTCG/TCTTCC`	1	--	int¹		4	NA EA	164
rs1875232^1,2	C,F,A,	56683911(+)	`tatcaG/Tctagg`	1	--	ng5¹		8	NS EA NA WA	514
rs1875233^1,2	C,F,A,	56686704(+)	`CAGATA/GGGTCA`	1	--	int¹		22	EA NA NS WA	1620
rs2013775^1,2	C,F,	56686380(+)	`TCTGAT/CGGCTG`	1	--	int¹		10	EA NS NA	2080
rs2070838^1,2	C,A,	56686002(+)	`AACTCC/GTTAAC`	1	--	int¹		8	NA EA WA	220
rs2070839^1,2	C,F,A,	56686242(+)	`TCCAGC/TCCCAG`	1	--	int¹		16	EA NA NS WA	2220
rs4619400^1,2	C,A,	56686005(+)	`TCCTTA/TACTTT`	1	--	int¹		9	NA EA WA	222
rs6499848^1,2	C,	56684953(+)	`GAGACA/G/TAATAT`	1	--	ng5¹		7	NA EA WA	218
rs6499849^1,2	C,F,A,	56687452(+)	`GATGAC/TGGGGT`	1	--	ng3¹		8	NA EA WA	356

About this table

HapMap Linkage Disequilibrium images for MT1B (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for MT1B: --

QIAGEN SeqTarget long-range PCR primers for resequencing MT1B

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

MT1B for disorders About GeneDecksing

OMIM: 156349

Export disorders and mutations for MT1B gene to outside databases

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶UniProtKB/TrEMBL, and/or ⁷Novoseek)
About This Section

10/13 PubMed articles for MT1B gene, integrated from 7 sources (see all 13):
(articles sorted by number of sources associating them with MT1B)

Structure and tissue-specific expression of the human metallothionein IB gene. (PubMed id 3785191)^{1, 2, 3} Heguy A....Karin M. (1986)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Human metallothionein genes are clustered on chromosome 16. (PubMed id 6089206)^{1, 3} Karin M....Shows T.B. (1984)
Pathways and genes differentially expressed in the motor cortex of patients with sporadic amyotrophic lateral sclerosis. (PubMed id 17244347)¹ Lederer C.W....Cavallaro S. (2007)
HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages. (PubMed id 12539042)¹ Izmailova E....Aldovini A. (2003)
Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line. (PubMed id 15747776)¹ Sun N.N....Witten M.L. (2003)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)
Human metallothionein genes: structure of the functional locus at 16q13. (PubMed id 2286373)¹ West A.K....Richards R.I. (1990)
[Metallothionein and its isoform genes expression in the human pancreatic cancer cell strains and their function] (PubMed id 16274045)⁷ Xie Y....Li L.J. (2005)
[Metallothionein isoforms gene expression induced by cadmium in human peripheral blood lymphocytes] (PubMed id 15952643)⁷ Chang X.L....Zhou Y.F. (2005)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 4490	HGNC: 7394	AceView: MT1BandMT1K	Ensembl:ENSG00000169688	euGenes: HUgn4490
ECgene: MT1B	H-InvDB: MT1B

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Intellectual Property for MT1B gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from XenneX, Inc.)

About This Section

Patent Information for MT1B gene:
Search GeneIP for patents involving MT1B

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
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	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of MT1B
	SeqTarget long-range PCR primers for resequencing MT1B
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of MT1B

	GenScript Custom Purified and Recombinant Proteins Services for MT1B		GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for MT1B
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MT1B Gene

protein-coding GIFtS: 44

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MT1B Gene

protein-coding GIFtS: 44

Aliases & Descriptionsfor MT1B gene

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Proteinsfor MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

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for MT1B gene

Publications
for MT1B gene

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for MT1B gene

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for MT1B gene