Aliases &
Descriptions
for NOX4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| NADPH oxidase 41 2 | | Kidney oxidase-12 3 | | KOX-11 2 3 | | Renal NAD(P)H-oxidase2 3 | | RENOX2 3 5 | | Kidney superoxide-producing NADPH oxidase2 3 | | KOX1 2 | | EC 1.6.3.-3 |
Export aliases for NOX4 gene to outside databasesPrevious GC identifers: GC11M091382 GC11M090606 GC11M089244 GC11M088747 GC11M088699 GC11M089057 |
Summaries
for NOX4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for NOX4:
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidasecomplex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes thereduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have beenimplicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth.A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multipletranscript variants.
UniProtKB/Swiss-Prot: NOX4_HUMAN, Q9NPH5Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex withCYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensorregulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play arole in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in thenucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4displays an increased activity. Isoform 5 and isoform 6 display reduced activity
Gene Wiki entry for NOX4 |
Genomic Views
for NOX4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the NOX4 gene promoter:
NF-kappaB AP-1 NF-kappaB1 c-Fos c-Jun p53 Pax-6 CUTL1 Bach1 E47
Other transcription factors
Search SABiosciences Chromatin IP Primers for NOX4
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 11q14.2-q21 Ensembl cytogenetic band: 11q14.3 HGNC cytogenetic band: 11q14.2-q21NOX4 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11M085294: view genomic region
(about GC identifiers)
Start:
|
85,294,642 bp from pter |
End:
|
85,468,503 bp from pter |
Size:
|
173,862 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr11- 89,057,524-89,225,392 |
RefSeq DNA sequence:- NC_000011.9 NT_167190.1
|
Proteins
for NOX4 gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: NOX4_HUMAN, Q9NPH5 (See
protein sequence)Recommended Name: NADPH oxidase 4 Size: 578 amino acids; 66932 Da
Subunit: Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizesCYBA/p22phox. Interacts with TLR4
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membraneprotein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membraneand focal adhesions. According to PubMed:15927447, may also localize to the nucleus
Developmental stage: Expressed in fetal kidney and fetal liver
Secondary accessions: A8K715 Q5K3R4 Q5K3R5 Q5K3R6 Q5K3R8 Q7Z7G3 Q86V92Alternative splicing: 7 isoforms: Q9NPH5-1 Q9NPH5-2 Q9NPH5-3 Q9NPH5-4 Q9NPH5-5 Q9NPH5-6 Q9NPH5-7 Explore the universe of human proteins at neXtProt for NOX4: NX_Q9NPH5
Post-translational modifications:
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function1
REFSEQ proteins (3 alternative transcripts):
NP_001137308.1 NP_001137309.1 NP_058627.1
ENSEMBL proteins: ENSP00000263317 ENSP00000365146 ENSP00000405705 ENSP00000344747 ENSP00000412446 ENSP00000376961
Human Recombinant Proteins
5/7 Gene Ontology (GO) cellular component terms (GO ID links to tree view) (see all 7): About this table
NOX4 for ontologies About GeneDecksing
Antibodies for NOX4:
Assays for NOX4: |
Protein
Domains/
Families
for NOX4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
NOX4 for domains About GeneDecksing
5/6 InterPro domains/families (see all 6):Graphical View of Domain Structure for InterPro Entry Q9NPH5ProtoNet protein and cluster: Q9NPH5 2 Blocks protein families: IPB000778 Cytochrome B-245 heavy chain signature IPB013130 Ferric reductase-like transmembrane component
UniProtKB/Swiss-Prot: NOX4_HUMAN, Q9NPH5Similarity: Contains 1 FAD-binding FR-type domainSimilarity: Contains 1 ferric oxidoreductase domain |
Gene Function
for NOX4 gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: NOX4_HUMAN, Q9NPH5Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex withCYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensorregulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play arole in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in thenucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4displays an increased activity. Isoform 5 and isoform 6 display reduced activityEnzyme regulation: Inhibited by plumbagin (By similarity). Activated by phorbol 12-myristate 13-acetate (PMA).Activated by insulin. Inhibited by diphenylene iodoniumInduction: By 7-ketocholesterol (at protein level)Enzyme Number (IUBMB): EC 1.6.3.-
5/9 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 9): About this table
NOX4 for ontologies About GeneDecksing
Animal Models: 7 MGI mutant phenotypes (inferred from 3 alleles ) (MGI details for Nox4):
NOX4 for phenotypes About GeneDecksing
|
Pathways & Interactions
for NOX4 gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
NOX4 for pathways About GeneDecksing
4  Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for NOX4
SABiosciences Pathway-Focused PCR Arrays including NOX4: PAHS-050A PAHS-094A
Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for NOX4
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NOX4
1 Interacting protein for NOX4 (ENSP000002633173) via UniProtKB, MINT, and/or STRINGAbout this table
5/9 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 9): About this table
NOX4 for ontologies About GeneDecksing
|
Drugs & Compounds
for NOX4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
NOX4 for compounds About GeneDecksing
 Browse Tocris compounds for NOX4
10/13  Novoseek chemical compound relationships for NOX4 gene (see all 13)
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| nadph |
89.8 |
120 |
16025326 (4), 11032835 (3), 19540572 (3), 15322091 (3) (see all 75) |
| diphenyleneiodonium |
81.1 |
3 |
11032835 (1), 18206249 (1), 16227320 (1) |
| apocynin |
78.3 |
2 |
17501721 (1), 18092937 (1) |
| superoxide |
69.2 |
37 |
19671250 (5), 11032835 (3), 10869423 (2), 15322091 (2) (see all 22) |
| oxygen |
69.1 |
39 |
18783311 (4), 17259440 (1), 19812163 (1), 10869423 (1) (see all 26) |
| 7-ketocholesterol |
61.8 |
6 |
15572675 (2), 17448905 (1), 19157829 (1) |
| n acetylcysteine |
48.5 |
4 |
18783311 (1), 18206249 (1), 18092937 (1) |
| h2o2 |
42.9 |
1 |
18849343 (1) |
| 8-isoprostane |
38.5 |
1 |
17259440 (1) |
| nitric oxide |
35.9 |
3 |
19074681 (1), 19129694 (1) |
About this table
|
Transcripts
for NOX4 gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for NOX4 gene (3 alternative transcripts): NM_001143836.1 NM_001143837.1 NM_016931.3 Additional cDNA sequence: AB041035.1 AF254621.1 AF261943.1 AJ704726.1 AJ704727.1 AJ704728.1 AJ704729.1 AK057189.1 AK291830.1 AK298323.1 AK298328.1 AK298357.1 AK298376.1 AK311381.1 AK311389.1 AY288918.1 BC040105.1 BC051371.1 8 DOTS entries: DT.408129 DT.120737878 DT.100754298 DT.40128676 DT.75145131 DT.120737898 DT.92419790 DT.120737886 24/50 AceView cDNA sequences (see all 50): BX282690 AY288918 AI624542 AA919129 BC040105 NM_016931 AF261943 AA456001 AI433445 AB041035 BM693080 AI241222 AW024435 AI253120 BP376400 BU730205 BP377863 AA876251 AW418588 CB963821 BC051371 AK057189 AI885681 AI284575
 highest scoring ESTs for NOX4:AB041035 AF254621 AF261943 AI742260 AI885681 AK057189 AW237557 AY288918 BC040105 BC051371 Unigene Cluster for NOX4: NADPH oxidase 4 Hs.371036 [show with all ESTs]Unigene Representative Sequence: NM_001143837
GeneLoc Exon Structure
6 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000263317(uc001pcu.2 uc001pct.2 uc009yvo.2 uc010rtu.1)
ENST00000375979(uc001pcw.2 uc001pcx.2 uc001pcy.2) ENST00000413594
ENST00000343727(uc001pcv.2 uc009yvp.2 uc009yvs.1) ENST00000424319(uc009yvr.2 uc010rtv.1 uc009yvq.2)
ENST00000393282
|
Expression
for NOX4 gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| NOX4 expression in normal and diseased human tissues
1 / 2 / 3 6 probe-sets matching NOX4 gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 55457_at2, 3
|
U95-B |
1 |
1.00 |
1.00 |
0.97 |
1.34 |
AI742260 |
0.80 |
1.00 |
0.91 |
1 |
| 77936_at2
|
U95-D |
1 |
0.81 |
1.00 |
0.93 |
0.70 |
-- |
-- |
-- |
-- |
-- |
| 236843_at2
|
U133-B |
1 |
1.00 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 219773_at2, 3
|
U133-A |
1 |
0.91 |
1.00 |
-- |
-- |
NM_016931 |
0.60 |
1.00 |
0.82 |
1 |
| 236843_at2
|
U133Plus2 |
1 |
1.00 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 219773_at2
|
U133Plus2 |
1 |
0.91 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
NOX4 for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: CACTGAATTC
SOURCE GeneReport for Unigene cluster: Hs.371036
UniProtKB/Swiss-Prot: NOX4_HUMAN, Q9NPH5Tissue specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level).Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelialcells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells
SABiosciences Expression via Pathway-Focused PCR Arrays including NOX4: PAHS-050A PAHS-094A
|
Orthologs
for NOX4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for NOX4 gene from 5/6 species (see all 6)
About this table Species with no ortholog for NOX4
ENSEMBL Gene Tree for NOX4 (if available) |
Paralogs
for NOX4 gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for NOX4 gene
- CYBB2 NOX12 NOX32 DUOX22 NOX52 DUOX12
NOX4 for paralogs About GeneDecksing
|
Genomic Variants
for NOX4 gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 11 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for NOX4 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for NOX4
2 CNVs: 6951 53361 | | QIAGEN SeqTarget long-range PCR primers for resequencing NOX4 |
|
Disorders & Mutations
for NOX4 gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
NOX4 for disorders About GeneDecksing
OMIM: 605261
8 Novoseek disease relationships for NOX4 gene
About this table
Human Genome Epidemiology (HuGE) Navigator: NOX4 (8 documents)
Export disorders and mutations for NOX4 gene to outside databases
|
Medical News
for NOX4 gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for NOX4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/162 PubMed articles for NOX4 gene, integrated from 7 sources (see all 162):
(articles sorted by number of sources associating them with NOX4)- Nox4 is critical for hypoxia-inducible factor 2-alpha transcriptional activity in von Hippel-Lindau-deficient renal cell carcinoma. (PubMed id 16230378)1, 2, 7 Maranchie J.K. and Zhan Y. (2005)
- Identification of renox, an NAD(P)H oxidase in kidney. (PubMed id 10869423)1, 2, 7 Geiszt M.... Leto T.L. (2000)
- Identification of novel Nox4 splice variants with impact on ROS levels in A549 cells. (PubMed id 15721269)1, 2, 7 Goyal P.... Haenze J. (2005)
- NAD(P)H oxidase 4 mediates transforming growth factor-beta1-induced differentiation of cardiac fibroblasts into myofibroblasts. (PubMed id 16179589)1, 2, 7 Cucoranu I.... Sorescu D. (2005)
- NAD(P)H oxidase Nox-4 mediates 7-ketocholesterol-induced endoplasmic reticulum stress and apoptosis in human aortic smooth muscle cells. (PubMed id 15572675)1, 2, 7 Pedruzzi E.... Ogier-Denis E. (2004)
- A novel superoxide-producing NAD(P)H oxidase in kidney. (PubMed id 11032835)1, 2, 7 Shiose A.... Sumimoto H. (2001)
- The NAD(P)H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction. (PubMed id 14966267)1, 2, 7 Mahadev K.... Goldstein B.J. (2004)
- Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells. (PubMed id 15155719)1, 2, 7 Vaquero E.C....Gukovskaya A.S. (2004)
- NOX4 as an oxygen sensor to regulate TASK-1 activity. (PubMed id 16019190)1, 2 Lee Y.-M....Park J.-W. (2006)
- Functional analysis of Nox4 reveals unique characteristics compared to other NADPH oxidases. (PubMed id 15927447)1, 2 Martyn K.D....Knaus U.G. (2006)
|
External Searches for NOX4 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NOX4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NOX4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NOX4 gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for NOX4 gene:
Search GeneIP for patents involving NOX4
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NOX4 gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
|
 | |
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| | | |
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 |  |  |  | |
| |  | Search Tocris compounds for NOX4 |
| |  |  |  |  | | | | |
 | | | ELISAs and CLIAs for NOX4 |
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