Aliases &
Descriptions
for PIWIL1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| piwi-like 1 (Drosophila)1 2 | | piwi homolog2 | | HIWI1 2 3 | | piwi-like protein 12 | | PIWI1 2 | | MIWI2 | | piwi (Drosophila)-like 11 | | |
Export aliases for PIWIL1 gene to outside databasesPrevious GC identifers: GC12P129251 GC12P130586 GC12P129175 GC12P129347 GC12P130822 |
Summaries
for PIWIL1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PIWIL1:
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containingboth PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translationalregulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewalcapacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding differentisoforms have been found for this gene. (provided by RefSeq)
UniProtKB/Swiss-Prot: PIWL1_HUMAN, Q96J94Function: Plays a central role during spermatogenesis by repressing transposable elements and prevent theirmobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates therepression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins andgovern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotideRNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeatedsequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in otherprocesses during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNAcleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for somemiRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator
Gene Wiki entry for PIWIL1 |
Genomic Views
for PIWIL1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PIWIL1 gene promoter:
Elk-1 ER-alpha AP-2rep FOXJ2 (long isoform) CP1A CP1C NF-Y NF-YA Arnt ATF-2
Other transcription factors
Search SABiosciences Chromatin IP Primers for PIWIL1
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for PIWIL1  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 12q24.33 Ensembl cytogenetic band: 12q24.33 HGNC cytogenetic band: 12q24.33PIWIL1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12P127807: view genomic region
(about GC identifiers)
Start:
|
127,807,883 bp from pter |
End:
|
127,841,997 bp from pter |
Size:
|
34,115 bases |
Orientation:
|
plus strand |
1 alternative location:
| Chr12+ 130,822,614-130,856,872 |
RefSeq DNA sequence:- NC_000012.11 NT_009755.19
|
Proteins
for PIWIL1 gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: PIWL1_HUMAN, Q96J94 (See
protein sequence)Recommended Name: Piwi-like protein 1 Size: 861 amino acids; 98603 Da
Subunit: Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. Thisinteraction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (whenmethylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9 (By similarity)
Subcellular location: Cytoplasm (By similarity). Note=Component of the meiotic nuage, also named P granule, agerm-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body (Bysimilarity)
Secondary accessions: A4F266 O95404 Q8NA60 Q8TBY5 Q96JD5Alternative splicing: 3 isoforms: Q96J94-1 Q96J94-2 Q96J94-3 Explore the universe of human proteins at neXtProt for PIWIL1: NX_Q96J94
Post-translational modifications:
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2,RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule (Bysimilarity)1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins (2 alternative transcripts):
NP_001177900.1 NP_004755.2
ENSEMBL proteins: ENSP00000245255
Human Recombinant Proteins
5 Gene Ontology (GO) cellular component terms (GO ID links to tree view): About this table
PIWIL1 for ontologies About GeneDecksing
Antibodies for PIWIL1:
Assays for PIWIL1: |
Protein
Domains/
Families
for PIWIL1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PIWIL1 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q96J94ProtoNet protein and cluster: Q96J94 2 Blocks protein families: IPB003100 Argonaute and Dicer protein IPB003165 Stem cell self-renewal protein Piwi
UniProtKB/Swiss-Prot: PIWL1_HUMAN, Q96J94Similarity: Belongs to the argonaute family. Piwi subfamilySimilarity: Contains 1 PAZ domainSimilarity: Contains 1 Piwi domain |
Gene Function
for PIWIL1 gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: PIWL1_HUMAN, Q96J94Function: Plays a central role during spermatogenesis by repressing transposable elements and prevent theirmobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates therepression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins andgovern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotideRNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeatedsequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in otherprocesses during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNAcleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for somemiRNAs stability (By similarity). Isoform 3 may be a negative developmental regulatorInduction: Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation
5 Gene Ontology (GO) molecular function terms (GO ID links to tree view): About this table
PIWIL1 for ontologies About GeneDecksing
Animal Models: 2 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Piwil1):
PIWIL1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for PIWIL1 gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
PIWIL1 for pathways About GeneDecksing
1  Kegg Pathway (Kegg details for PIWIL1):
SABiosciences Custom Pathway-Focused PCR Arrays for PIWIL1
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PIWIL1
4 Interacting proteins for PIWIL1 (Q96J941 ENSP000002452553) via UniProtKB, MINT, and/or STRINGAbout this table
5/8 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 8): About this table
PIWIL1 for ontologies About GeneDecksing
|
Drugs & Compounds
for PIWIL1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for PIWIL1
|
Transcripts
for PIWIL1 gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for PIWIL1 gene (2 alternative transcripts): NM_001190971.1 NM_004764.4 Additional cDNA sequence: AB274731.1 AF104260.2 AF264004.1 AF387507.1 AK093133.1 BC028581.2 6 DOTS entries: DT.405949 DT.100001500 DT.97790109 DT.303336 DT.121169040 DT.97825156 24/56 AceView cDNA sequences (see all 56): AA639672 BF092403 AA969660 NM_004764 AK093133 BX281582 AI208149 AX747944 AA774856 AL041473 BC028581 AL705072 BM728018 BU732873 AA757218 AF104260 BM680592 BI459464 BI463897 BG718168 BG721550 AI125224 BF092519 AL704986
 highest scoring ESTs for PIWIL1:AF104260 AA639672 AA904973 AA969660 AF264004 AF387507 AI125224 AI538315 AI698821 AL044133 Unigene Cluster for PIWIL1: Piwi-like 1 (Drosophila) Hs.405659 [show with all ESTs]Unigene Representative Sequence: NM_004764
GeneLoc Exon Structure
5/9 Alternative Splicing Database (ASD) splice patterns (SP) for PIWIL1 (see all 9)
| ExUns: | 1 | ^ | 2a | · | 2b | · | 2c | · | 2d | · | 2e | ^ | 3 | ^ | 4a | · | 4b | · | 4c | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b | · | 8c | · | 8d | · | 8e | · | 8f | · | 8g | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | ^ |
|---|
|
| SP1: | | | | | | | | | - | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | - | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | - | | - | | - | | - | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | - | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | - | | - | | - | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| ExUns: | 15 | ^ | 16 | ^ | 17 | ^ | 18 | ^ | 19 | ^ | 20 | ^ | 21 | ^ | 22 | ^ | 23 | ^ | 24a | · | 24b | ^ | 25 |
|---|
|
| SP1: | | | | | | | | | | | | | | | - | | | | | | - | | | | |
| SP2: | | | | | | | | | | | | | | | - | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for PIWIL1
1 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000245255(uc001uij.1 uc001uik.2)
|
Expression
for PIWIL1 gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| PIWIL1 expression in normal and diseased human tissues
1 / 2 / 3 5 probe-sets matching PIWIL1 gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 35901_at2, 3
|
U95-A |
1 |
0.75 |
1.00 |
0.05 |
0.34 |
AF104260 |
1.00 |
1.00 |
1.00 |
1 |
| 76330_r_at2, 3
|
U95-D |
1 |
0.38 |
1.00 |
0.91 |
1.23 |
AA889526 |
0.60 |
1.00 |
0.82 |
1 |
| 76328_i_at2, 3
|
U95-D |
1 |
0.31 |
1.00 |
0.72 |
0.78 |
AA889526 |
0.60 |
1.00 |
0.82 |
1 |
| 214868_at2, 3
|
U133-A |
1 |
0.73 |
1.00 |
-- |
-- |
AW189518 |
0.80 |
1.00 |
0.91 |
1 |
| 214868_at2
|
U133Plus2 |
1 |
0.73 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
PIWIL1 for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: ATTCTATTTG
SOURCE GeneReport for Unigene cluster: Hs.405659
Expression variation in blood from EXPOLDB for PIWIL1 UniProtKB/Swiss-Prot: PIWL1_HUMAN, Q96J94Tissue specificity: Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells;detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originatingfrom embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somaticorigin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitouslyexpressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells
SABiosciences Custom PCR Arrays for PIWIL1
|
Orthologs
for PIWIL1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for PIWIL1 gene from 5/10 species (see all 10)
About this table Species with no ortholog for PIWIL1
ENSEMBL Gene Tree for PIWIL1 (if available) |
Paralogs
for PIWIL1 gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for PIWIL1 gene
- PIWIL42 PIWIL22 EIF2C22 EIF2C42 PIWIL32 EIF2C12 EIF2C32
PIWIL1 for paralogs About GeneDecksing
|
Genomic Variants
for PIWIL1 gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 12 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for PIWIL1 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 5 variations for PIWIL1
5 CNVs: 71624 8740 3000 3901 35062 | | QIAGEN SeqTarget long-range PCR primers for resequencing PIWIL1 |
|
Disorders & Mutations
for PIWIL1 gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PIWIL1 for disorders About GeneDecksing
OMIM: 605571
Human Genome Epidemiology (HuGE) Navigator: PIWIL1 (4 documents)
Export disorders and mutations for PIWIL1 gene to outside databases
|
Medical News
for PIWIL1 gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for PIWIL1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/23 PubMed articles for PIWIL1 gene, integrated from 7 sources (see all 23):
(articles sorted by number of sources associating them with PIWIL1)- Identification of eight members of the Argonaute family in the human genome. (PubMed id 12906857)1, 2, 3 Sasaki T.... Shimizu N. (2003)
- Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi. (PubMed id 11154219)1, 2, 7 Sharma A.K.... Hoffman R. (2001)
- Expression of hiwi gene in human gastric cancer was associated with proliferation of cancer cells. (PubMed id 16287078)1, 2, 7 Liu X.... Shou C. (2006)
- The induction of H3K9 methylation by PIWIL4 at the p16(Ink4a) locus. (PubMed id 17544373)1, 2 Sugimoto K.... Takai D. (2007)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. (PubMed id 14749716)1, 2 Tahbaz N....Hobman T.C. (2004)
- Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas. (PubMed id 12037681)1, 2 Qiao D.... Lin H. (2002)
- A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal. (PubMed id 9851978)1, 3 Cox D.N....Lin H. (1998)
- Expression of PSCA, PIWIL1, and TBX2 in endometrial a denocarcinoma. (PubMed id 20502058)1, 7 Liu W.K....Zhang Z.X. (2010)
|
External Searches for PIWIL1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PIWIL1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PIWIL1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PIWIL1 gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for PIWIL1 gene:
Search GeneIP for patents involving PIWIL1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PIWIL1 gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
|
 | |
 |
| | | |
 |
 |
| | |
 |  |  |  | |
| |  | Search Tocris compounds for PIWIL1 |
| |  |  |  |  | | | | |
 | | |
|
|
