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POLR2B Gene

protein-coding   GIFtS: 63

GC04P053798
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
(Previous name: polymerase (RNA) II (DNA directed) polypeptide B (140kD) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa1 2     hsRPB22
RPB21 2     hRPB1402
RNA polymerase II subunit 22 3     DNA-directed RNA polymerase II subunit RPB22
DNA-directed RNA polymerase II 140 kDa polypeptide2 3     OTTHUMP000001589732
DNA-directed RNA polymerase II subunit B2 3     OTTHUMP000002189692
RNA polymerase II subunit B22 3     DNA directed RNA polymerase II 140 kDa polypeptide, RNA polymerase II subunit
2, RNA polymerase II second largest subunit2
POL2RB2 5     OTTHUMP000002018272
polymerase (RNA) II (DNA directed) polypeptide B (140kD)1     EC 2.7.7.63

External Ids:    HGNC: 91881   Entrez Gene: 54312   Ensembl: ENSG000000473157   UniProtKB: P308763   

Export aliases for POLR2B gene to outside databases

Previous GC identifers: GC04P057648 GC04P057841 GC04P057760 GC04P057685 GC04P057538


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for POLR2B:
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a
structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and
the newly synthesized RNA. (provided by RefSeq)

UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside
triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many
functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase
active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II
transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core
element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are
thought to grab the incoming DNA template (By similarity)

Gene Wiki entry for POLR2B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the POLR2B gene promoter:
         RP58   POU3F2   Meis-1   HOXA9B   STAT5A   STAT2   STAT1beta   STAT1alpha   STAT1   STAT3   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for POLR2B

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q12   Ensembl cytogenetic band:  4q12   HGNC cytogenetic band: 4q12

POLR2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
POLR2B gene location

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P053798:  view genomic region     (about GC identifiers)

Start:
53,798,541 bp from pter
End:
53,850,908 bp from pter
Size:
52,368 bases
Orientation:
plus strand

1 alternative location:
Chr4+ 57,843,888-57,897,324     
RefSeq DNA sequence:
NC_000004.11  NT_022853.15  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876 (See protein sequence)
Recommended Name: DNA-directed RNA polymerase II subunit RPB2  
Size: 1174 amino acids; 133897 Da
Subunit: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits
Subcellular location: Nucleus
Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably
involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion
coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity)
Secondary accessions: A8K1A8 Q8IZ61

Explore the universe of human proteins at neXtProt for POLR2B: NX_P30876 

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_000929.1  

    ENSEMBL proteins: 
    ENSP00000407728 ENSP00000379541 ENSP00000312735 ENSP00000391452 ENSP00000391096 ENSP00000408638 
    ENSP00000370625 


    Human Recombinant Proteins 
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    GenScript Custom Purified and Recombinant Proteins Services for POLR2B 
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins

    3 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA9852112
    GO:0005654 nucleoplasm TAS--
    GO:0005665 DNA-directed RNA polymerase II, core complex IDA9852112
    About this table

    POLR2B for ontologies           About GeneDecksing



    Antibodies for POLR2B: 
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for POLR2B
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    GenScript Custom Superior Antibodies Services for POLR2B 
    Novus Biologicals Antibodies for POLR2B
    Epitomics antibodies for POLR2B

    Assays for POLR2B: 
    Browse Kits and Assays available from Millipore
    Browse ELISAs at Sigma-Aldrich
    OriGene Custom Immunoassay Development 
    Browse OriGene Fluorogenic Cell Assay Kits 
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for POLR2B 
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for POLR2B 


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    POLR2B for domains           About GeneDecksing

    5/10 InterPro domains/families (see all 10):
     IPR007120 DNA-dir_RNA_pol_su2_6
     IPR007641 RNA_pol_Rpb2_7
     IPR007644 RNA_pol_bsu_protrusion
     IPR015712 DNA-dir_RNA_pol_su2
     IPR007645 RNA_pol_Rpb2_3

    Graphical View of Domain Structure for InterPro Entry P30876

    ProtoNet protein and cluster: P30876

    4 Blocks protein families:
    IPB007120 RNA polymerase Rpb2
    IPB007121 RNA polymerase
    IPB007646 RNA polymerase Rpb2
    IPB007647 RNA polymerase Rpb2


    UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876
    Similarity: Belongs to the RNA polymerase beta chain family


    (According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
    About This Section

    UniProtKB/Swiss-Prot: RPB2_HUMAN, P30876
    Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside
    triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many
    functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase
    active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II
    transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core
    element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are
    thought to grab the incoming DNA template (By similarity)
    Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
    Enzyme Number (IUBMB): EC 2.7.7.6 

    Inhib.
    RNA:
        
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for POLR2B
    Sigma-Aldrich shRNA for POLR2B
    OriGene 29mer shRNA kits in GFP-retroviral vector: POLR2B
    OriGene shRNA RFP: POLR2B
    OriGene basic RS shRNA: POLR2B
    OriGene siRNA: POLR2B
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of POLR2B 
    miRNA:Sigma-Aldrich microRNA Mimics for POLR2B
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of POLR2B 
    1 SABiosciences Assays for microRNA that regulate POLR2B:
    hsa-miR-1283

    Gene
    Editing:
    Browse CompoZr Knockout ZFN at Sigma-Aldrich 

    Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
    Browse iPSC Reprogramming Factors at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: POLR2B
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: POLR2B
    OriGene untagged cDNA clones in CMV expression vector: POLR2B
    OriGene 3'-UTR clone: POLR2B 
    Browse MicroRNA Expression Plasmids 
    GenScript cDNA clone with any tag delivered in your preferred vector: POLR2B 
    Browse Sino Biological Human cDNA Clones

    Cell
    Lines:
      
    GenScript Custom overexpressing Cell Line Services for POLR2B 

    Genatlas biochemistry entry for POLR2B:
    polymerase II,RNA (DNA directed) large polypeptide B (140kDa)

    5/8 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 8):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0003899 DNA-directed RNA polymerase activity IEA--
    GO:0004672contributes to protein kinase activity IDA9852112
    GO:0005515 protein binding IPI17353931
    GO:0016740 transferase activity IEA--
    About this table

    POLR2B for ontologies           About GeneDecksing


    (Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
    About This Section


    POLR2B for pathways           About GeneDecksing

    3 Millipore Pathways for POLR2B
        Transcription Ligand-Dependent Transcription of Retinoid-Target genes
    DNA damage Role of Brca1 and Brca2 in DNA repair
    ATP/ITP metabolism

    5/9 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  POLR2B (see all 9)
        Pyrimidine Metabolism
    Huntington's Disease Signaling
    Hereditary Breast Cancer Signaling
    Estrogen Receptor Signaling
    CREB Signaling in Neurons

    5 Kegg Pathways  (Kegg details for POLR2B):
        hsa00230 Purine metabolism
    hsa00240 Pyrimidine metabolism
    hsa01100 Metabolic pathways
    hsa03020 RNA polymerase
    hsa05016 Huntington's disease

        SABiosciences Custom Pathway-Focused PCR Arrays for POLR2B 

        Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for POLR2B
        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for POLR2B

    5/254 Interacting proteins for POLR2B (ENSP000003706253 P308761, 2) via UniProtKB, MINT, and/or STRING (see all 254)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CTDP1Q9Y5B02STRING: ENSP00000299543 MINT-51646 MINT-51650
    MAGI2Q86UL81EBI-394712,EBI-311035
    WWOXQ9NZC72MINT-72766
    POLR2CENSP000002192523STRING: ENSP00000219252
    POLR2DENSP000002726453STRING: ENSP00000272645
    About this table

    5/13 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 13):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000398 nuclear mRNA splicing, via spliceosome TAS--
    GO:0006281 DNA repair TAS--
    GO:0006283 transcription-coupled nucleotide-excision repair TAS--
    GO:0006289 nucleotide-excision repair TAS--
    GO:0006350 transcription TAS--
    About this table

    POLR2B for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section
    Browse Small Molecules at Sigma-Aldrich
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for POLR2B


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Millipore,
    siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
    Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
    About This Section

    Inhib.
    RNA:
         
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for POLR2B
    Sigma-Aldrich shRNA for POLR2B
    OriGene 29mer shRNA kits in GFP-retroviral vector: POLR2B
    OriGene shRNA RFP: POLR2B
    OriGene basic RS shRNA: POLR2B
    OriGene siRNA: POLR2B
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of POLR2B 
    miRNA: Sigma-Aldrich microRNA Mimics for POLR2B
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of POLR2B 
    1 SABiosciences Assays for microRNA that regulate POLR2B:
    hsa-miR-1283
    Clones: Browse FLAG tag genes at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: POLR2B
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: POLR2B
    OriGene untagged cDNA clones in CMV expression vector: POLR2B
    OriGene 3'-UTR Clone: POLR2B 
    Browse OriGene MicroRNA Expression Plasmids 
    GenScript cDNA clone with any tag delivered in your preferred vector: POLR2B 
    Primers: OriGene genome-wide validated SYBR primer pairs: POLR2B
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for POLR2B: PPH12742A

    REFSEQ mRNAs for POLR2B gene: 

    NM_000938.1  

    Additional cDNA sequence: 

    AF055028.1 AK098271.1 AK289823.1 AK294901.1 AK295132.1 AK299090.1 BC023503.2 BC046499.1 
    CR591583.1 CR592056.1 CR609388.1 X63563.1 

    24/31 DOTS entries (see all 31):

    DT.100674865  DT.97760173  DT.65285131  DT.100674870  DT.100706253  DT.91806226  DT.121219395  DT.40205446 
    DT.92040454  DT.95170547  DT.95170564  DT.100045783  DT.95278266  DT.121219269  DT.121219296  DT.121219317 
    DT.100639411  DT.101978157  DT.121219282  DT.121219386  DT.91916126  DT.92461397  DT.92461407  DT.95278263 

    24/414 AceView cDNA sequences (see all 414):

    BU628845 BU195049 BE550741 BM818298 AF055028 CA429196 BU615587 AI992183 
    AA001828 BM673648 BU183822 AI093559 CK431060 BQ771577 BM769676 BQ001027 
    BQ001562 AA376849 BQ020611 BE645837 AI801273 BQ215678 AL043215 CR592056 

    highest scoring ESTs for POLR2B:

    X63563 AA001828 AA002032 AA076596 AA232818 AA255492 AA256916 AA258348 AA258505 AA455376 

    Unigene Cluster for POLR2B:

    Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
    Hs.602757  [show with all ESTs]
    Unigene Representative Sequence: AK295132


    GeneLoc Exon Structure

    5/15 Alternative Splicing Database (ASD) splice patterns (SP) for POLR2B (see all 15)

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12a ·
    SP1:                          -                                                                                                                                 
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 12b ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b ^ 25 ^ 26
    SP1:                                                                                                                                    
    SP2:                                                                                                                                    
    SP3:                                                                                                                                    
    SP4:                                                                                                                                    
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for POLR2B
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000484821 ENST00000433463 ENST00000396241 ENST00000314595 ENST00000441246(uc011cae.1)
    ENST00000431623 ENST00000478188(uc003hcm.1) ENST00000495311 ENST00000510355
    ENST00000464918 ENST00000497845 ENST00000450656 ENST00000381227(uc003hcl.1 uc011caf.1)


    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Primers from OriGene and/or SABiosciences )
    About This Section

    POLR2B expression in normal and diseased human tissues

    1  / 2  / 3

    5 probe-sets matching POLR2B gene

    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    39746_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 X63563 1.00 0.90 0.95 1
    201803_at2, 3 U133-A 1 1.00 1.00 -- -- NM_000938 0.60 1.00 0.82 1
    1555837_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    201803_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    1555836_at*2 U133Plus2 1 0.46 1.00 -- -- -- -- -- -- --
    About this table

    POLR2B for expression           About GeneDecksing

    Data from Genenote  (Publications) and GNF BioGPS
        About these images
    POLR2B gene expression
    POLR2B gene electronic northern expression
    POLR2B gene sage expression
    About these images

    CGAP SAGE TAG: CTTTTCTTCT

    SOURCE GeneReport for Unigene cluster: Hs.602757

    Expression variation in blood from EXPOLDB for POLR2B

    Primers: OriGene genome-wide validated SYBR primer pairs: POLR2B
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for POLR2B: PPH12742A
        SABiosciences Custom PCR Arrays for POLR2B 


    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section

    Orthologs for POLR2B gene from 5/26 species (see all 26)
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    POLR2B1   -- polymerase (RNA) II (DNA directed) polypeptide B, 14 more 94.92(n)
    99.91(a)
    475152  XM_855837.1  XP_860930.1 
    chimpanzee
    (Pan troglodytes)
    POLR2B1   -- polymerase (RNA) II (DNA directed) polypeptide B, 14 more 99.66(n)
    100(a)
    461305  XM_517275.2  XP_517275.2 
    cow
    (Bos taurus)
    POLR2B1   -- polymerase (RNA) II (DNA directed) polypeptide B, 14 more 94.32(n)
    99.91(a)
    538184  XM_618380.3  XP_618380.3 
    rat
    (Rattus norvegicus)
    Polr2b1   -- polymerase (RNA) II (DNA directed) polypeptide B, 14 more 89.89(n)
    100(a)
    289561  XM_214021.4  XP_214021.3 
    mouse
    (Mus musculus)
    Polr2b1 , 5 5 (41.66 cM)5
    polymerase (RNA) II (DNA directed) polypeptide B1, 5 90.37(n)1
    99.91(a)1
    2313291  NM_153798.11  NP_722493.11 
     AA5010425  AK0397665  (see all 12)
    About this table        Species with no ortholog for POLR2B

    ENSEMBL Gene Tree for POLR2B (if available)

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section
    Paralogs for POLR2B gene
    POLR3B2  

    POLR2B for paralogs           About GeneDecksing



    (SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
    About This Section

    10/545 NCBI SNPs in POLR2B are shown (see all 545)
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ----------
    rs10563641,2
    C,F,A,H,57889677(-) GTCTCA/GCTAGT 2 S syn1 ref1 ese322Minor allele frequency- G:0.32MN EA NS NA 2918
    rs12772791,2
    C,H,57865403(+) CTCTCA/TTCTAT 1 -- int1 trp36Minor allele frequency- T:0.17NA WA 12
    rs12772801,2
    C,A,H,57866872(+) TTTTTT/GGGGGG 1 -- int14Minor allele frequency- G:0.35NA WA 80
    rs12772811,2
    C,F,O,H,57856092(-) AAAAGT/CAGACA 1 -- int119Minor allele frequency- C:0.07MN NS EA NA WA 862
    rs12772821,2
    C,F,O,H,57855819(-) GAGACT/CTTCTT 1 -- int127Minor allele frequency- C:0.07MN NS EA NA WA 1820
    rs12772831,2
    C,F,O,H,57855641(-) ACCAAT/AATTAG 1 -- int121Minor allele frequency- A:0.07MN NS EA NA WA 1344
    rs12772841,2
    C,F,O,A,H,57855361(-) ACTTTA/CCCCAA 1 -- int126Minor allele frequency- N:0.00MN EA NA NS WA 3220
    rs12772851,2
    C,H,57854486(-) CCTTCG/TATTTT 1 -- int110Minor allele frequency- T:0.08NA EA WA 228
    rs12772861,2
    C,F,O,H,57854298(-) TCACCC/ATCAAC 1 -- int111Minor allele frequency- A:0.10NA WA 274
    rs12772871,2
    C,F,A,H,57853147(-) acatgC/Gcatga 1 -- int110Minor allele frequency- G:0.25NS EA NA WA 636
    About this table

    HapMap Linkage Disequilibrium images for POLR2B (up to first 250kb)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for POLR2B: --

    QIAGEN SeqTarget long-range PCR primers for resequencing POLR2B 

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    POLR2B for disorders           About GeneDecksing

    OMIM: 180661

    Human Genome Epidemiology (HuGE) Navigator: POLR2B (3 documents)

    Export disorders and mutations for POLR2B gene to outside databases

    (Possibly Related Articles in Doctor's Guide)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
    About This Section

    10/144 PubMed articles for POLR2B gene, integrated from 7 sources (see all 144):
    (articles sorted by number of sources associating them with POLR2B)
    1. Primary structure of the second largest subunit of human RNA polymerase II (or B). (PubMed id 1518060)1, 2, 3 Acker J.... Kedinger C. (1992)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. (PubMed id 9852112)1, 2 Kershnar E.... Chiang C.-M. (1998)
    4. Large-scale concatenation cDNA sequencing. (PubMed id 9110174)1, 2 Yu W.... Gibbs R.A. (1997)
    5. Chromosomal localization of human RNA polymerase II subunit genes. (PubMed id 8034326)1, 3 Acker J....Kedinger C. (1994)
    6. Identification of proteins interacting with the RNAPII FCP1 phosphatase: FCP1 forms a complex with arginine methyltransferase PRMT5 and it is a substrate for PRMT5-mediated methylation. (PubMed id 15670829)1, 7 Amente S....Majello B. (2005)
    7. An atlas of combinatorial transcriptional regulation in mouse and man. (PubMed id 20211142)1 Ravasi T....Hayashizaki Y. (2010 Mar 5)
    8. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    9. Aire's partners in the molecular control of immunolog ical tolerance. (PubMed id 20085707)1 Abramson J....Mathis D. (2010)
    10. MRE11 complex links RECQ5 helicase to sites of DNA damage. (PubMed id 19270065)1 Zheng L....Janscak P. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 5431 HGNC: 9188 AceView: POLR2B Ensembl:ENSG00000047315 euGenes: HUgn5431
    ECgene: POLR2B Kegg: 5431 H-InvDB: POLR2B

    (According to HUGE)
    About This Section
      --

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
      --

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from XenneX, Inc.)
    About This Section
    Patent Information for POLR2B gene:
    Search GeneIP for patents involving POLR2B

    GeneCards and IP:
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