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PPP1R3D Gene

protein-coding   GIFtS: 49

GC20M055299
protein phosphatase 1, regulatory (inhibitor) subunit 3D
(Previous names: protein phosphatase 1, regulatory subunit 3D )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: PPP1R6)
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
protein phosphatase 1, regulatory (inhibitor) subunit 3D1 2     PP1 subunit R62 3
PPP1R62 3 5     protein phosphatase 1, regulatory subunit 6, spinophilin2
protein phosphatase 1, regulatory subunit 3D1 2     DKFZp781L24412
Protein phosphatase 1 regulatory subunit 62 3     protein phosphatase 1 regulatory subunit 3D2
Protein phosphatase 1-binding subunit R62 3     

External Ids:    HGNC: 92941   Entrez Gene: 55092   Ensembl: ENSG000001328257   UniProtKB: O956853   

Export aliases for PPP1R3D gene to outside databases

Previous GC identifers: GC20M058242 GC20M058201 GC20M059150 GC20M059199 GC20M059197 GC20M057945 GC20M058511


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for PPP1R3D:
Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular
functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation
is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of
4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates
with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity.
Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of
PP1. (provided by RefSeq)

UniProtKB/Swiss-Prot: PPR3D_HUMAN, O95685
Function: Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in
the regulation of glycogen metabolism, muscle contractility and protein synthesis


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the PPP1R3D gene promoter:
         p300   CREB   ATF-2   E4BP4   SRF   CUTL1   Arnt   RSRFC4   TGIF   ATF   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for PPP1R3D

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for PPP1R3D 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.3   Ensembl cytogenetic band:  20q13.33   HGNC cytogenetic band: 20q13.3

PPP1R3D Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
PPP1R3D gene location

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M055299:  view genomic region     (about GC identifiers)

Start:
55,299,100 bp from pter
End:
55,302,565 bp from pter
Size:
3,466 bases
Orientation:
minus strand

1 alternative location:
Chr20- 58,511,894-58,515,352     
RefSeq DNA sequence:
NC_000020.10  NT_011362.10  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: PPR3D_HUMAN, O95685 (See protein sequence)
Recommended Name: Protein phosphatase 1 regulatory subunit 3D  
Size: 299 amino acids; 32559 Da
Subunit: Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen
Secondary accessions: Q6DK02

Explore the universe of human proteins at neXtProt for PPP1R3D: NX_O95685 

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006233.1  

    ENSEMBL proteins: 
    ENSP00000360035 


    Human Recombinant Proteins 
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    Uscn ELISAs and CLIAs for PPP1R3D 


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    PPP1R3D for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR017434 Pase-1_Glycogen_target-su_met
     IPR005036 CBM_21

    Graphical View of Domain Structure for InterPro Entry O95685

    ProtoNet protein and cluster: O95685

    1 Blocks protein family: IPB005036 Putative phosphatase regulatory subunit

    UniProtKB/Swiss-Prot: PPR3D_HUMAN, O95685
    Domain: The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some
    regulatory subunit of phosphatase complexes
    Similarity: Contains 1 CBM21 (carbohydrate binding type-21) domain


    (According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
    About This Section

    UniProtKB/Swiss-Prot: PPR3D_HUMAN, O95685
    Function: Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in
    the regulation of glycogen metabolism, muscle contractility and protein synthesis

    Inhib.
    RNA:
        
    Browse for Gene Knock-down Tools from Millipore
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    OriGene siRNA: PPP1R3D
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    miRNA:Sigma-Aldrich microRNA Mimics for PPP1R3D
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of PPP1R3D 
    8/12 SABiosciences Assays for microRNAs that regulate PPP1R3D (see all 12):
    hsa-miR-29c hsa-miR-29a hsa-miR-192 hsa-miR-182 hsa-miR-124 hsa-miR-633 hsa-miR-506 hsa-miR-1201

    Gene
    Editing:
    Browse CompoZr Knockout ZFN at Sigma-Aldrich 

    Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
    Browse iPSC Reprogramming Factors at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene untagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene 3'-UTR clone: PPP1R3D 
    Browse MicroRNA Expression Plasmids 
    GenScript cDNA clone with any tag delivered in your preferred vector: PPP1R3D 
    Browse Sino Biological Human cDNA Clones

    Cell
    Lines:
      
    GenScript Custom overexpressing Cell Line Services for PPP1R3D 

    2 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004722 protein serine/threonine phosphatase activity TAS9414128
    GO:0005515 protein binding IPI17474147
    About this table

    PPP1R3D for ontologies           About GeneDecksing


    (Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
    About This Section


    PPP1R3D for pathways           About GeneDecksing

    5/9 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  PPP1R3D (see all 9)
        Protein Kinase A Signaling
    Production of Nitric Oxide and Reactive Oxygen Species in Macrophages
    Cardiac β-adrenergic Signaling
    ERK/MAPK Signaling
    Dopamine Receptor Signaling

    1 Kegg Pathway  (Kegg details for PPP1R3D):
        hsa04910 Insulin signaling pathway

        SABiosciences Custom Pathway-Focused PCR Arrays for PPP1R3D 

        Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for PPP1R3D
        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for PPP1R3D

    2 Interacting proteins for PPP1R3D (O956851, 2) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    FYNP062411, 2EBI-1045661,EBI-515315 MINT-7246726
    SFNP319472MINT-3975555
    About this table

    2 Gene Ontology (GO) biological process terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975 carbohydrate metabolic process IEA--
    GO:0005977 glycogen metabolic process IEA--
    About this table

    PPP1R3D for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section
    Browse Small Molecules at Sigma-Aldrich
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for PPP1R3D


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Millipore,
    siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
    Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
    About This Section

    Inhib.
    RNA:
         
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich siRNA Panels and esiRNA and siRNA for PPP1R3D
    Sigma-Aldrich shRNA Panels and shRNA for PPP1R3D
    OriGene 29mer shRNA kits in GFP-retroviral vector: PPP1R3D
    OriGene shRNA RFP: PPP1R3D
    OriGene basic RS shRNA: PPP1R3D
    OriGene siRNA: PPP1R3D
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of PPP1R3D 
    miRNA: Sigma-Aldrich microRNA Mimics for PPP1R3D
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of PPP1R3D 
    8/12 SABiosciences Assays for microRNAs that regulate PPP1R3D (see all 12):
    hsa-miR-29c hsa-miR-29a hsa-miR-192 hsa-miR-182 hsa-miR-124 hsa-miR-633 hsa-miR-506 hsa-miR-1201
    Clones: Browse FLAG tag genes at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene untagged cDNA clones in CMV expression vector: PPP1R3D
    OriGene 3'-UTR Clone: PPP1R3D 
    Browse OriGene MicroRNA Expression Plasmids 
    GenScript cDNA clone with any tag delivered in your preferred vector: PPP1R3D 
    Primers: OriGene genome-wide validated SYBR primer pairs: PPP1R3D
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for PPP1R3D: PPH09167A

    REFSEQ mRNAs for PPP1R3D gene: 

    NM_006242.3  

    Additional cDNA sequence: 

    BC047502.1 BC074860.2 BC074861.2 BX647298.1 Y18206.1 

    1 DOTS entry:

    DT.443807 

    24/77 AceView cDNA sequences (see all 77):

    AI338933 AI075344 AA227932 AA326551 AI458545 AA723361 AA227617 AI680750 
    BX119062 BC074861 AW873990 CK825369 BC074860 NM_006242 BF939036 AI015117 
    AI700308 BQ270649 AI590073 AI472595 AI702893 AI368076 CB215770 BQ894406 

    highest scoring ESTs for PPP1R3D:

    Y18206 AI018630 AI680750 AL532901 AW365038 BC047502 BE840620 R10183 AA227617 AA227932 

    Unigene Cluster for PPP1R3D:

    Protein phosphatase 1, regulatory (inhibitor) subunit 3D
    Hs.42215  [show with all ESTs]
    Unigene Representative Sequence: BC047502


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000370996(uc002ybb.2)

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Primers from OriGene and/or SABiosciences )
    About This Section

    PPP1R3D expression in normal and diseased human tissues

    1  / 2  / 3

    6 probe-sets matching PPP1R3D gene

    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    38948_at2, 3 U95-A 1 0.62 1.00 0.80 0.76 Y18206 1.00 1.00 1.00 1
    45191_at2, 3 U95-B 1 0.38 1.00 0.95 1.24 AI680750 0.80 1.00 0.91 1
    204555_s_at2, 3 U133-A 1 0.64 1.00 -- -- NM_006242 0.60 1.00 0.82 1
    204554_at2 U133-A 1 0.36 1.00 -- -- -- -- -- -- --
    204555_s_at2 U133Plus2 1 0.64 1.00 -- -- -- -- -- -- --
    204554_at2 U133Plus2 1 0.36 1.00 -- -- -- -- -- -- --
    About this table

    PPP1R3D for expression           About GeneDecksing

    Data from Genenote  (Publications) and GNF BioGPS
        About these images
    PPP1R3D gene expression
    PPP1R3D gene electronic northern expression
    PPP1R3D gene sage expression
    About these images

    CGAP SAGE TAG: TAGAGAATCT

    SOURCE GeneReport for Unigene cluster: Hs.42215

    Expression variation in blood from EXPOLDB for PPP1R3D

    UniProtKB/Swiss-Prot: PPR3D_HUMAN, O95685
    Tissue specificity: Expressed in all tissue tested. High expression in skeletal muscle and heart

    Primers: OriGene genome-wide validated SYBR primer pairs: PPP1R3D
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for PPP1R3D: PPH09167A
        SABiosciences Custom PCR Arrays for PPP1R3D 


    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section

    Orthologs for PPP1R3D gene from 5/8 species (see all 8)
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    PPP1R3D1   -- protein phosphatase 1, regulatory (inhibitor) subunit more 89.07(n)
    86.96(a)
    485953  XM_543078.1  XP_543078.1 
    chimpanzee
    (Pan troglodytes)
    PPP1R3D1   -- protein phosphatase 1, regulatory (inhibitor) subunit more 99(n)
    98.66(a)
    469992  XM_001142110.1  XP_001142110.1 
    cow
    (Bos taurus)
    PPP1R3D1   -- protein phosphatase 1, regulatory (inhibitor) subunit more 87.96(n)
    88.29(a)
    782160  XM_001250296.1  XP_001250297.1 
    rat
    (Rattus norvegicus)
    Ppp1r3d1   -- protein phosphatase 1, regulatory subunit 3D 81.36(n)
    85.66(a)
    689995  XM_001072850.1  XP_001072850.1 
    mouse
    (Mus musculus)
    Ppp1r3d1 , 5 2 (99.91 cM)5
    protein phosphatase 1, regulatory subunit 3D1, 5 81.84(n)1
    86.38(a)1
    2289661  XM_141580.31  XP_141580.11 
     AI8512835  AK0115395 
    About this table        Species with no ortholog for PPP1R3D

    ENSEMBL Gene Tree for PPP1R3D (if available)

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section
    Paralogs for PPP1R3D gene
    PPP1R3G2  PPP1R3A2  PPP1R3B2  PPP1R3E2  PPP1R3C2  

    PPP1R3D for paralogs           About GeneDecksing



    (SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
    About This Section
    QIAGEN SeqTarget long-range PCR primers for resequencing PPP1R3D 

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    PPP1R3D for disorders           About GeneDecksing

    OMIM: 603326


    Export disorders and mutations for PPP1R3D gene to outside databases

    (Possibly Related Articles in Doctor's Guide)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
    About This Section

    10 PubMed articles for PPP1R3D gene integrated from 7 sources:
    (articles sorted by number of sources associating them with PPP1R3D)
    1. PPP1R6, a novel member of the family of glycogen-targeting subunits of protein phosphatase 1. (PubMed id 9414128)1, 2, 3 Armstrong C.G.... Cohen P.T.W. (1997)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
    4. Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines. (PubMed id 9275233)1, 3 Allen P.B....Greengard P. (1997)
    5. Phosphoproteome of resting human platelets. (PubMed id 18088087)2 Zahedi R.P.... Sickmann A. (2008)
    6. A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (2008)
    7. Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. (PubMed id 17474147)1 Wu C....Li S.S. (2007)
    8. Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer. (PubMed id 15778465)1 Benzinger A.... Hermeking H. (2005)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    10. Genetic alterations and expression of the protein phosphatase 1 genes in human cancers. (PubMed id 11251179)7 Takakura S....Yokota J. (2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 5509 HGNC: 9294 AceView: PPP1R3D Ensembl:ENSG00000132825 euGenes: HUgn5509
    ECgene: PPP1R3D Kegg: 5509 H-InvDB: PPP1R3D

    (According to HUGE)
    About This Section
      --

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
      --

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from XenneX, Inc.)
    About This Section
    Patent Information for PPP1R3D gene:
    Search GeneIP for patents involving PPP1R3D

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
    Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
    About This Section

     Millipore Custom Antibody & Bulk Services
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     Regulatory tfbs in PPP1R3D promoter
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     ELISAs and CLIAs for PPP1R3D
           

    GeneCards Homepage - Last full update: 31 Dec 2010 - Incremental updates: 9 Jan 2011 , 10 Jan 2011

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