PPP1R9B Gene
protein-coding GIFtS: 52
GC17M043579
|
|
protein phosphatase 1, regulatory (inhibitor) subunit 9B
(Previous names: protein phosphatase 1, regulatory subunit 9B, spinophilin )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: PPP1R6, PPP1R9)
|
Aliases &
Descriptions
for PPP1R9B gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| protein phosphatase 1, regulatory (inhibitor) subunit 9B1 2 | | neurabin-22 | | protein phosphatase 1, regulatory subunit 9B, spinophilin1 2 | | PPP1R92 | | Spn1 2 | | neurabin II2 | | SPINO1 2 | | neurabin-II2 | | Protein phosphatase 1 regulatory subunit 9B2 3 | | spinophilin2 | | PPP1R62 3 | | Spinophilin3 | | FLJ303452 | | Neurabin-II3 |
Export aliases for PPP1R9B gene to outside databasesPrevious GC identifers: GC17U990076 GC17M050692 GC17M048553 GC17M048687 GC17M048685 GC17M045567 GC17M048211 |
Summaries
for PPP1R9B gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for PPP1R9B:
Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highlyenriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory inputin the central nervous system (Allen et al., 1997 (PubMed 9275233)).(supplied by OMIM)
UniProtKB/Swiss-Prot: NEB2_HUMAN, Q96SB3Function: Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmissionand dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along thesides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at thesynaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, andregulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamatereceptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors andalpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptorsby linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediatesregulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effectorproteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity)
Gene Wiki entry for PPP1R9B |
Genomic Views
for PPP1R9B gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the PPP1R9B gene promoter:
CREB deltaCREB NRSF form 2 NRSF form 1 JunB Fra-1 c-Jun JunD AP-1 c-Fos
Other transcription factors
Search SABiosciences Chromatin IP Primers for PPP1R9B
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for PPP1R9B  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 17q21.33 Ensembl cytogenetic band: 17q21.33 HGNC cytogenetic band: 17q21.33PPP1R9B Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 17 GeneLoc Exon Structure GeneLoc location for GC17M043579: view genomic region
(about GC identifiers)
Start:
|
43,578,978 bp from pter |
End:
|
43,595,756 bp from pter |
Size:
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16,779 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr17- 48,211,104-48,227,991 |
RefSeq DNA sequence:- NC_000017.10 NT_010783.15
|
Proteins
for PPP1R9B gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: NEB2_HUMAN, Q96SB3 (See
protein sequence)Recommended Name: Neurabin-2 Size: 815 amino acids; 89192 Da
Subunit: Possibly exists as an homodimer, homotrimer or an homotetramer. Interacts with F-actin, PPP1CA, neurabin-1,TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A,PPP1R2, RASGFR1 and TIAM1
Subcellular location: Cytoplasm, cytoskeleton (By similarity). Nucleus (By similarity). Cell projection, dendriticspine (By similarity). Cell junction, synapse. Note=Enriched at synapse and cadherin-based cell-cell adhesion sites.In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed toexitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendriticspines (By similarity)
Secondary accessions: Q8TCR9Explore the universe of human proteins at neXtProt for PPP1R9B: NX_Q96SB3
Post-translational modifications:
Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 ismediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association withF-actin, but does not affect its binding to PP1 (By similarity)1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_115984.3
Human Recombinant Proteins
5/9 Gene Ontology (GO) cellular component terms (GO ID links to tree view) (see all 9): About this table
PPP1R9B for ontologies About GeneDecksing
Antibodies for PPP1R9B:
Assays for PPP1R9B: |
Protein
Domains/
Families
for PPP1R9B gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
PPP1R9B for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q96SB3ProtoNet protein and cluster: Q96SB3 2 Blocks protein families: IPB000515 Binding-protein-dependent transport systems inner membrane component IPB001478 PDZ/DHR/GLGF domain
UniProtKB/Swiss-Prot: NEB2_HUMAN, Q96SB3Similarity: Contains 1 PDZ (DHR) domain |
Gene Function
for PPP1R9B gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: NEB2_HUMAN, Q96SB3Function: Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmissionand dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along thesides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at thesynaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, andregulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamatereceptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors andalpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptorsby linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediatesregulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effectorproteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity)
4 Gene Ontology (GO) molecular function terms (GO ID links to tree view): About this table
PPP1R9B for ontologies About GeneDecksing
Animal Models: 4 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Ppp1r9b):
PPP1R9B for phenotypes About GeneDecksing
|
Pathways & Interactions
for PPP1R9B gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
SABiosciences Custom Pathway-Focused PCR Arrays for PPP1R9B
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for PPP1R9B
5/7 Interacting proteins for PPP1R9B (Q96SB32) via UniProtKB, MINT, and/or STRING (see all 7)About this table
5/9 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 9): About this table
PPP1R9B for ontologies About GeneDecksing
|
Drugs & Compounds
for PPP1R9B gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
PPP1R9B for compounds About GeneDecksing
 Browse Tocris compounds for PPP1R9B
4  Novoseek chemical compound relationships for PPP1R9B gene
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| letrozole |
63.8 |
6 |
15229239 (2), 16502389 (2), 16344155 (1), 20097718 (1) |
| dopamine |
37.5 |
4 |
16462601 (1), 10194355 (1), 16199832 (1), 17157277 (1) |
| glutamate |
33.3 |
7 |
17279777 (2), 15217898 (1), 15728359 (1), 12230305 (1) (see all 6) |
| estradiol |
28.7 |
2 |
12351732 (1), 15229239 (1) |
About this table
|
Transcripts
for PPP1R9B gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for PPP1R9B gene: NM_032595.3 Additional cDNA sequence: AJ401189.1 AK054907.1 AL157449.1 AL713642.1 BC008564.1 BC011232.1 BC016162.1 CR605734.1 5 DOTS entries: DT.312456 DT.95243515 DT.97868666 DT.100044479 DT.95072296 24/135 AceView cDNA sequences (see all 135): AI475690 BF726761 BM695932 AI056581 BM991997 BC008564 AW451413 BC011232 AI242565 BU620207 AL157449 CO246370 BE646343 AI251854 AI766708 BM460087 CA944794 BE895415 BM548779 BI912507 BM718161 BG393753 NM_032595 AI621262
 highest scoring ESTs for PPP1R9B:AI523759 AI797774 AI808223 AI969706 AJ401189 AL713642 AW028349 AW302139 BF109903 BF448737 Unigene Cluster for PPP1R9B: Protein phosphatase 1, regulatory (inhibitor) subunit 9B Hs.514323 [show with all ESTs]Unigene Representative Sequence: NM_032595
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for PPP1R9B
| ExUns: | 1 | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 | ^ | 5a | · | 5b | ^ | 6a | · | 6b | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10a | · | 10b |
|---|
|
| SP1: | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for PPP1R9B
3 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000316878(uc002iqh.3) ENST00000501501 ENST00000513579
|
Expression
for PPP1R9B gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| PPP1R9B expression in normal and diseased human tissues
1 / 2 / 3 6 probe-sets matching PPP1R9B gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 52900_at2, 3
|
U95-B |
1 |
0.94 |
1.00 |
0.77 |
1.36 |
AI797774 |
0.80 |
1.00 |
0.91 |
1 |
| 70964_at2, 3
|
U95-E |
1 |
0.62 |
1.00 |
0.76 |
0.64 |
AI523759 |
1.00 |
1.00 |
1.00 |
1 |
| 225124_at2, 3
|
U133-B |
1 |
0.91 |
1.00 |
-- |
-- |
BF508705 |
0.60 |
1.00 |
0.82 |
1 |
| 230455_at2, 3
|
U133-B |
1 |
0.73 |
1.00 |
-- |
-- |
AI523759 |
1.00 |
1.00 |
1.00 |
1 |
| 225124_at2
|
U133Plus2 |
1 |
0.91 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 230455_at2
|
U133Plus2 |
1 |
0.73 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
PPP1R9B for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: CCCTCTGTCA
SOURCE GeneReport for Unigene cluster: Hs.514323
SABiosciences Custom PCR Arrays for PPP1R9B
|
Orthologs
for PPP1R9B gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for PPP1R9B gene from 4 species
About this table Species with no ortholog for PPP1R9B
ENSEMBL Gene Tree for PPP1R9B (if available) |
Paralogs
for PPP1R9B gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| -- |
Genomic Variants
for PPP1R9B gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 17 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for PPP1R9B (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for PPP1R9B
2 CNVs: 88525 77656 | | QIAGEN SeqTarget long-range PCR primers for resequencing PPP1R9B |
|
Disorders & Mutations
for PPP1R9B gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
PPP1R9B for disorders About GeneDecksing
OMIM: 603325
5 Novoseek disease relationships for PPP1R9B gene
About this table
Human Genome Epidemiology (HuGE) Navigator: PPP1R9B (1 document)
Export disorders and mutations for PPP1R9B gene to outside databases
|
Medical News
for PPP1R9B gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for PPP1R9B gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/81 PubMed articles for PPP1R9B gene, integrated from 7 sources (see all 81):
(articles sorted by number of sources associating them with PPP1R9B)- The human tumor suppressor ARF interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein. (PubMed id 11278317)1, 2, 7 Vivo M.... La Mantia G. (2001)
- Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines. (PubMed id 9275233)1, 3, 7 Allen P.B....Greengard P. (1997)
- Phosphorylation of spinophilin modulates its interaction with actin filaments. (PubMed id 12417592)1, 7 Hsieh-Wilson L.C....Greengard P. (2003)
- Scaffold proteins IRSp53 and spinophilin regulate loc alized Rac activation by T-lymphocyte invasion and metastasis protein 1 (TIAM1) . (PubMed id 20360004)1, 7 Rajagopal S....Buchsbaum R.J. (2010)
- Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5). (PubMed id 15728359)1, 7 Futter M....Nairn A.C. (2005)
- Identification of neurabin II as a novel doublecortin interacting protein. (PubMed id 14550532)1, 7 Tsukada M....Eichele G. (2003)
- The actin-binding domain of spinophilin is necessary and sufficient for targeting to dendritic spines. (PubMed id 12230305)1, 7 Grossman S.D....Greengard P. (2002)
- Characterization of the neuronal targeting protein spinophilin and its interactions with protein phosphatase-1. (PubMed id 10194355)1, 7 Hsieh-Wilson L.C....Greengard P. (1999)
- PPP1R9B (Neurabin 2): involvement and dynamics in the NK immunological synapse. (PubMed id 19130477)1, 7 Meng X....Wilkins J.A. (2009)
- Regulation of p70 S6 kinase by complex formation between the Rac guanine nucleotide exchange factor (Rac-GEF) Tiam1 and the scaffold spinophilin. (PubMed id 12531897)1, 7 Buchsbaum R.J....Feig L.A. (2003)
|
External Searches for PPP1R9B gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing PPP1R9B gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing PPP1R9B gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing PPP1R9B gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for PPP1R9B gene:
Search GeneIP for patents involving PPP1R9B
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for PPP1R9B gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
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