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RASGRP1 Gene

protein-coding   GIFtS: 58

GC15M015624
RAS guanyl releasing protein 1 (calcium and DAG-regulated)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
RAS guanyl releasing protein 1 (calcium and DAG-regulated)1 2     guanine nucleotide exchange factor, calcium- and DAG-regulated, Rap1A2
RASGRP1 2 3     CALDAG-GEFI2
CalDAG-GEFII1 3     ras activator RasGRP2
Calcium and DAG-regulated guanine nucleotide exchange factor II2 3     CALDAG-GEFII2
RAS guanyl-releasing protein 12     RAS guanyl nucleotide-releasing protein 12
MGC1299982     MGC1299992
V2     Ras guanyl-releasing protein3
hRasGRP12     

External Ids:    HGNC: 98781   Entrez Gene: 101252   Ensembl: ENSG000001725757   UniProtKB: O952673   

Export aliases for RASGRP1 gene to outside databases

Previous GC identifers: GC15M034123 GC15M031676 GC15M036359 GC15M036496 GC15M036567 GC15M038780


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for RASGRP1:
This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide
exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically
activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates
T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding
different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause
of susceptibility to systemic lupus erythematosus (SLE). (provided by RefSeq)

UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267
Function: Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through
the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes
antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and
differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating
allergic responses. May also function in other cell types differentiation

Gene Wiki entry for RASGRP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RASGRP1 gene promoter:
         NF-kappaB1   NF-kappaB   GATA-1   TGIF   MEF-2A   c-Myb   PPAR-gamma2   PPAR-gamma1   FOXL1   POU2F1   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for RASGRP1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for RASGRP1 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q14   Ensembl cytogenetic band:  15q14   HGNC cytogenetic band: 15q15

RASGRP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RASGRP1 gene location

GeneLoc gene densities for chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15M015624:  view genomic region     (about GC identifiers)

Start:
15,624,949 bp from pter
End:
15,701,560 bp from pter
Size:
76,612 bases
Orientation:
minus strand

1 alternative location:
Chr15- 38,780,308-38,857,007     
RefSeq DNA sequence:
NC_000015.9  NT_010194.17  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267 (See protein sequence)
Recommended Name: RAS guanyl-releasing protein 1  
Size: 797 amino acids; 90402 Da
Subunit: Forms a signaling complex with DGKZ and HRAS. Interacts with F-actin. Interacts with SKAP1
Subcellular location: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgi apparatus membrane;
Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Found both in the
cytosol and associated with membranes. Relocalization to the cell membrane upon activation is F-actin-dependent.
Translocates to the Golgi in response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but
not to axons of hippocampal pyramidal cells (By similarity)
Developmental stage: Expressed in fetal brain and kidney
Sequence caution: Sequence=AAH67298.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A
sequence;
Secondary accessions: Q56CZ0 Q58G75 Q59HB1 Q5I3A8 Q6GV31 Q6NX39 Q7LDG6 Q9UI94 Q9UNN9
Alternative splicing: 5 isoforms:  O95267-1   O95267-2   O95267-3   O95267-4   O95267-5   (Additional isoforms seem to exist. Several splicing events may be used independently in a modular way)

Explore the universe of human proteins at neXtProt for RASGRP1: NX_O95267 

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001122074.1  NP_005730.2  


    ENSEMBL proteins: 
    ENSP00000414301 ENSP00000388540 ENSP00000413105 ENSP00000310244 ENSP00000395257 


    Human Recombinant Proteins 
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein: RASGRP1
    OriGene Protein Over-expression Lysate: RASGRP1
    GenScript Custom Purified and Recombinant Proteins Services for RASGRP1 
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins

    5/9 Gene Ontology (GO) cellular component terms (GO ID links to tree view) (see all 9):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005622 intracellular IEA--
    GO:0005624 membrane fraction TAS9582122
    GO:0005737 cytoplasm IEA--
    GO:0005783 endoplasmic reticulum IEA--
    About this table

    RASGRP1 for ontologies           About GeneDecksing



    Antibodies for RASGRP1: 
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    GenScript Custom Superior Antibodies Services for RASGRP1 
    Browse antibodies at Epitomics

    Assays for RASGRP1: 
    Browse Kits and Assays available from Millipore
    Browse ELISAs at Sigma-Aldrich
    OriGene Custom Immunoassay Development 
    Browse OriGene Fluorogenic Cell Assay Kits 
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for RASGRP1 
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for RASGRP1 


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    RASGRP1 for domains           About GeneDecksing

    5/8 InterPro domains/families (see all 8):
     IPR008937 Ras_GEF
     IPR011992 EF-hand-like_dom
     IPR020454 DAG/PE-bd
     IPR018249 EF_HAND_2
     IPR001895 RasGRF_CDC25

    Graphical View of Domain Structure for InterPro Entry O95267

    ProtoNet protein and cluster: O95267

    1 Blocks protein family: IPB001895 Guanine-nucleotide dissociation stimulator CDC25

    UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267
    Domain: The phorbol-ester/DAG-type zinc finger is the principal mediator of the targeting to membranes and is required
    for functional activation through DAG-binding
    Similarity: Belongs to the RASGRP family
    Similarity: Contains 2 EF-hand domains
    Similarity: Contains 1 N-terminal Ras-GEF domain
    Similarity: Contains 1 phorbol-ester/DAG-type zinc finger
    Similarity: Contains 1 Ras-GEF domain


    (According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
    About This Section

    UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267
    Function: Functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through
    the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Couples T-lymphocytes and B-lymphocytes
    antigen receptors to the activation of Ras. Hence, regulates T-cells and B-cells development, homeostasis and
    differentiation. Functions also in FcERI-evoked degranulation and cytokine secretion by mast cells, regulating
    allergic responses. May also function in other cell types differentiation
    Enzyme regulation: Probably regulated by calcium (By similarity). Regulated by PLC gamma, DGKA, DGKZ and by F-actin
    polymerization

    Inhib.
    RNA:
        
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for RASGRP1
    Sigma-Aldrich shRNA for RASGRP1
    OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): RASGRP1
    OriGene shRNA RFP (see all 2): RASGRP1
    OriGene basic RS shRNA (see all 2): RASGRP1
    OriGene siRNA (see all 2): RASGRP1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of RASGRP1 
    miRNA:Sigma-Aldrich microRNA Mimics for RASGRP1
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of RASGRP1 
    8/16 SABiosciences Assays for microRNAs that regulate RASGRP1 (see all 16):
    hsa-miR-98 hsa-let-7d hsa-miR-548k hsa-let-7c hsa-let-7i hsa-miR-21 hsa-let-7g hsa-let-7e

    Gene
    Editing:
    Browse CompoZr Knockout ZFN at Sigma-Aldrich 

    Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
    Browse iPSC Reprogramming Factors at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: RASGRP1
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): RASGRP1
    OriGene untagged cDNA clones in CMV expression vector (see all 2): RASGRP1
    OriGene 3'-UTR clone (see all 2): RASGRP1 
    Browse MicroRNA Expression Plasmids 
    GenScript Custom cDNA clone Services for RASGRP1 
    Browse Sino Biological Human cDNA Clones

    Cell
    Lines:
      
    GenScript Custom overexpressing Cell Line Services for RASGRP1 

    Genatlas biochemistry entry for RASGRP1:
    guanine nucleotide releasing (exchange) protein 1,RAS activator,calcium and DAG-regulated,expressed in the nervous
    system

    4 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005085 guanyl-nucleotide exchange factor activity TAS--
    GO:0005509 calcium ion binding IEA--
    GO:0005515 protein binding IPI17353931
    GO:0008289 lipid binding TAS9582122
    About this table

    RASGRP1 for ontologies           About GeneDecksing

    Animal Models: 4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Rasgrp1):

    hematopoietic systemimmune systemliver/biliary systemrenal/urinary system

    RASGRP1 for phenotypes           About GeneDecksing


    (Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
    About This Section


    RASGRP1 for pathways           About GeneDecksing

    5/7 Millipore Pathways for RASGRP1 (see all 7)
        Development Beta-adrenergic receptors regulation of ERK
    G-protein signaling K-RAS regulation pathway
    G-protein signaling N-RAS regulation pathway
    G-protein signaling M-RAS regulation pathway
    G-protein signaling TC21 regulation pathway

    3 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for  RASGRP1
        G-Protein Coupled Receptor Signaling
    T Cell Receptor Signaling
    Molecular Mechanisms of Cancer

    2 Kegg Pathways  (Kegg details for RASGRP1):
        hsa04010 MAPK signaling pathway
    hsa04660 T cell receptor signaling pathway

        SABiosciences Custom Pathway-Focused PCR Arrays for RASGRP1 

        Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for RASGRP1
        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RASGRP1

    5/39 Interacting proteins for RASGRP1 (O952671, 2 ENSP000003102443) via UniProtKB, MINT, and/or STRING (see all 39)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PPM1BO756881EBI-1054956,EBI-1047039
    VHLP403371EBI-1054956,EBI-301246
    CACNB4ENSP000002019433STRING: ENSP00000343563 STRING: ENSP00000201943
    CACNA1AENSP000003533623STRING: ENSP00000353362
    CACNA1BENSP000002775493STRING: ENSP00000277549
    About this table

    5/8 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 8):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction TAS9789079
    GO:0007264 small GTPase mediated signal transduction ----
    GO:0007265 Ras protein signal transduction TAS9582122
    GO:0007596 blood coagulation TAS--
    GO:0023034 intracellular signaling pathway ----
    About this table

    RASGRP1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    RASGRP1 for compounds           About GeneDecksing

    Browse Small Molecules at Sigma-Aldrich
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for RASGRP1
    3 Novoseek chemical compound relationships for RASGRP1 gene
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    bryostatin 1 76.2 3 15588099 (1), 10779365 (1), 10807788 (1)
    calcium 31.2 14 9582122 (3), 9789079 (2), 10807788 (2), 11503142 (1) (see all 7)
    lipid 1.16 2 17523924 (1), 10779365 (1)
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Millipore,
    siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
    Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
    About This Section

    Inhib.
    RNA:
         
    Browse for Gene Knock-down Tools from Millipore
    Browse Nano Scale siRNA at Sigma-Aldrich
    Sigma-Aldrich esiRNA and siRNA for RASGRP1
    Sigma-Aldrich shRNA for RASGRP1
    OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): RASGRP1
    OriGene shRNA RFP (see all 2): RASGRP1
    OriGene basic RS shRNA (see all 2): RASGRP1
    OriGene siRNA (see all 2): RASGRP1
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of RASGRP1 
    miRNA: Sigma-Aldrich microRNA Mimics for RASGRP1
    Browse microRNA target validation systems at Sigma-Aldrich 
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of RASGRP1 
    8/16 SABiosciences Assays for microRNAs that regulate RASGRP1 (see all 16):
    hsa-miR-98 hsa-let-7d hsa-miR-548k hsa-let-7c hsa-let-7i hsa-miR-21 hsa-let-7g hsa-let-7e
    Clones: Browse FLAG tag genes at Sigma-Aldrich
    OriGene GFP tagged cDNA clones in CMV expression vector: RASGRP1
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): RASGRP1
    OriGene untagged cDNA clones in CMV expression vector (see all 2): RASGRP1
    OriGene 3'-UTR Clone (see all 2): RASGRP1 
    Browse OriGene MicroRNA Expression Plasmids 
    GenScript Custom cDNA clone Services for RASGRP1 
    Primers: OriGene genome-wide validated SYBR primer pairs: RASGRP1
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for RASGRP1: PPH13989A

    REFSEQ mRNAs for RASGRP1 gene (2 alternative transcripts): 

    NM_001128602.1  NM_005739.3  

    Additional cDNA sequence: 

    AB208848.1 AF081195.1 AF081197.1 AF106071.1 AK295097.1 AK314091.1 AK316067.1 AY634315.2 
    AY858556.2 AY954625.4 AY966005.1 BC032849.1 BC067298.1 BC109296.1 BC109297.1 

    10 DOTS entries:

    DT.452069  DT.91786977  DT.121054866  DT.95257426  DT.121054783  DT.40197398  DT.100861292  DT.91744312 
    DT.91744313  DT.210193 

    24/79 AceView cDNA sequences (see all 79):

    AI698379 BM704085 AA884877 Z43707 AA394197 AA043684 BC067298 AA278696 
    Z39766 AA278633 AA043685 AF081197 BU729996 AW965045 BG744494 AA731879 
    AA283882 AA251642 BM918122 AA742351 BQ049446 AA688183 BX456903 BG778473 

    highest scoring ESTs for RASGRP1:

    AF081195 AF106071 BC032849 BC067298 BI459385 CB989807 CB990554 CD244627 AA017173 AA021527 

    Unigene Cluster for RASGRP1:

    RAS guanyl releasing protein 1 (calcium and DAG-regulated)
    Hs.591127  [show with all ESTs]
    Unigene Representative Sequence: AF081195


    GeneLoc Exon Structure

    5/11 Alternative Splicing Database (ASD) splice patterns (SP) for RASGRP1 (see all 11)

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12 ^ 13 ^ 14a · 14b ^ 15a · 15b ^ 16 ^ 17 ^
    SP1:                          -                                                                                                                       -         
    SP2:                          -                                                                                               -     -                 -         
    SP3:                          -                                                                                               -     -                 -     -   
    SP4:                          -                                                                                               -     -                 -         
    SP5:              -     -     -                                                                                                                                 

    ExUns: 18 ^ 19 ^ 20a · 20b ^ 21a · 21b · 21c
    SP1:                                          
    SP2:                                          
    SP3:                                          
    SP4:              -     -                     
    SP5:                                          

    About this scheme

    ECgene alternative splicing isoforms for RASGRP1
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000431814 ENST00000450598(uc010bbe.2 uc010bbf.2 uc010bbg.2 uc001zkd.3)
    ENST00000414708 ENST00000310803(uc001zke.3) ENST00000415523

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Primers from OriGene and/or SABiosciences )
    About This Section

    RASGRP1 expression in normal and diseased human tissues

    1  / 2  / 3

    3 probe-sets matching RASGRP1 gene

    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33291_at2, 3 U95-A 1 0.69 1.00 1.00 1.00 AF081195 1.00 1.00 1.00 1
    205590_at2, 3 U133-A 1 0.82 1.00 -- -- NM_005739 0.60 1.00 0.82 1
    205590_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    About this table

    RASGRP1 for expression           About GeneDecksing

    Data from Genenote  (Publications) and GNF BioGPS
        About these images
    RASGRP1 gene expression
    RASGRP1 gene electronic northern expression
    RASGRP1 gene sage expression
    About these images

    CGAP SAGE TAG: GGGCTAGTGG

    SOURCE GeneReport for Unigene cluster: Hs.591127

    Expression variation in blood from EXPOLDB for RASGRP1

    UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267
    Tissue specificity: Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in
    the hematopoietic system. Expressed in T-cells (at protein level)

    Primers: OriGene genome-wide validated SYBR primer pairs: RASGRP1
    Browse OriGene validated miRNA SYBR primer pairs 
    SABiosciences RT2 qPCR Primer Assay for RASGRP1: PPH13989A
        SABiosciences Custom PCR Arrays for RASGRP1 


    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section

    Orthologs for RASGRP1 gene from 5/11 species (see all 11)
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    RASGRP11   -- RAS guanyl releasing protein 1 (calcium and DAG-regulated) 91.42(n)
    95.47(a)
    478255  XM_852583.1  XP_857676.1 
    chimpanzee
    (Pan troglodytes)
    RASGRP11   -- RAS guanyl releasing protein 1 (calcium and DAG-regulated) 99.75(n)
    99.87(a)
    453316  XM_510293.2  XP_510293.2 
    cow
    (Bos taurus)
    RASGRP11   -- RAS guanyl releasing protein 1 (calcium and DAG-regulated) 90.88(n)
    94.1(a)
    533125  XM_001253077.1  XP_001253078.1 
    rat
    (Rattus norvegicus)
    Rasgrp11   -- RAS guanyl releasing protein 1 (calcium and DAG-regulated) 87.05(n)
    90.16(a)
    29434  NM_019211.2  NP_062084.2 
    mouse
    (Mus musculus)
    Rasgrp11 , 5 2 (59.19 cM)5
    RAS guanyl releasing protein 11, 5 86.8(n)1
    90.67(a)1
    194191  NM_011246.21  NP_035376.11 
     AF1060705  AI8497925  (see all 14)
    About this table        Species with no ortholog for RASGRP1

    ENSEMBL Gene Tree for RASGRP1 (if available)

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section
    Paralogs for RASGRP1 gene
    RASGRP42  RASGRP32  RASGRP22  

    RASGRP1 for paralogs           About GeneDecksing



    (SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
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    10/657 NCBI SNPs in RASGRP1 are shown (see all 657)
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidChr 15 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ----------
    rs9679051,2
    --38853056(-) GACTCG/TTAAGC 1 -- int10--------
    rs11369731,2
    C,F,38781312(-) AGCCAA/GTAGCA 1 -- ut31 ese315Minor allele frequency- N:0.00MN EA NA WA 978
    rs11369741,2
    --38781188(-) CACTGA/GGTTTT 1 -- ut31 ese31Minor allele frequency- G:0.00MN 184
    rs18024411,2
    --38781687(-) AATGTG/TGAAGT 1 -- ut310--------
    rs39356411,2
    C,F,38798465(+) GGTGTG/CTATAG 1 -- int111Minor allele frequency- C:0.08NA NS EA 724
    rs39361221,2
    C,F,38791283(-) ATTAGG/AAATGT 1 -- int120Minor allele frequency- A:0.07NS EA NA 1570
    rs40758041,2
    C,F,A,38827933(-) TTCAAC/TTGCTC 1 -- int113Minor allele frequency- T:0.13NS EA NA WA 1040
    rs41323801,2
    H38811705(-) GGCCAG/CAAAGA 1 -- int14Minor allele frequency- C:0.00NS EA 418
    rs43137701,2
    C,F38782493(+) TTTTAG/TATCGC 1 -- ut318Minor allele frequency- T:0.01NS EA NA 554
    rs43342571,2
    C,F,H,38791826(+) GAACTC/ATGCTG 1 -- int126Minor allele frequency- A:0.28NS EA NA WA 1700
    About this table

    HapMap Linkage Disequilibrium images for RASGRP1 (up to first 250kb)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for RASGRP1: --

    QIAGEN SeqTarget long-range PCR primers for resequencing RASGRP1 

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
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    RASGRP1 for disorders           About GeneDecksing

    OMIM: 603962

    UniProtKB/Swiss-Prot: GRP1_HUMAN, O95267
  • Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is
  • a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the
    skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the
    autoimmune system. Note=Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE
    patients raising the possibility that dysregulation of this signaling protein contributes to the development of
    autoimmunity in a subset of SLE patients

    Human Genome Epidemiology (HuGE) Navigator: RASGRP1 (4 documents)

    Export disorders and mutations for RASGRP1 gene to outside databases


    (Possibly Related Articles in Doctor's Guide)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
    About This Section

    10/56 PubMed articles for RASGRP1 gene, integrated from 7 sources (see all 56):
    (articles sorted by number of sources associating them with RASGRP1)
    1. A Rap guanine nucleotide exchange factor enriched highly in the basal ganglia. (PubMed id 9789079)1, 2, 3, 7 Kawasaki H.... Graybiel A.M. (1998)
    2. RasGRP, a Ras activator: mouse and human cDNA sequences and chromosomal positions. (PubMed id 10087292)1, 2, 3, 7 Bottorff D.A.... Stone J.C. (1999)
    3. Defective expression of ras guanyl nucleotide-releasing protein 1 in a subset of patients with systemic lupus erythematosus. (PubMed id 17878389)1, 2, 7 Yasuda S.... Koike T. (2007)
    4. Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism. (PubMed id 11257115)1, 2, 7 Topham M.K. and Prescott S.M. (2001)
    5. SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1. (PubMed id 17658605)1, 2 Kosco K.A....Mustelin T. (2008)
    6. Phospholipase Cgamma activates Ras on the Golgi apparatus by means of RasGRP1. (PubMed id 12845332)1, 2 Bivona T.G.... Philips M.R. (2003)
    7. Diacylglycerols containing Omega 3 and Omega 6 fatty acids bind to RasGRP and modulate MAP kinase activation. (PubMed id 14583629)1, 7 Madani S....Charkaoui-Malki M. (2004)
    8. RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. (PubMed id 9582122)1, 7 Ebinu J.O....Stone J.C. (1998)
    9. Unusual interplay of two types of Ras activators, RasGRP and SOS, establishes sensitive and robust Ras activation in lymphocytes. (PubMed id 17283063)1, 7 Roose J.P....Weiss A. (2007)
    10. Expression of a catalytically inactive form of diacylglycerol kinase alpha induces sustained signaling through RasGRP. (PubMed id 11919165)2, 7 Jones D.R.... Merida I. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 10125 HGNC: 9878 AceView: RASGRP1 Ensembl:ENSG00000172575 euGenes: HUgn10125
    ECgene: RASGRP1 Kegg: 10125 H-InvDB: RASGRP1

    (According to HUGE)
    About This Section
      --

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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      --

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from XenneX, Inc.)
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    Patent Information for RASGRP1 gene:
    Search GeneIP for patents involving RASGRP1

    GeneCards and IP:
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