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RSAD1 Gene

protein-coding   GIFtS: 44

GC17P043924
radical S-adenosyl methionine domain containing 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
radical S-adenosyl methionine domain containing 11 2
FLJ111641 2
Oxygen-independent coproporphyrinogen-III oxidase-like protein RSAD12 3
FLJ209752
radical S-adenosyl methionine domain-containing protein 1, mitochondrial2
EC 1.3.99.-3

External Ids:    HGNC: 256341   Entrez Gene: 553162   Ensembl: ENSG000001364447   UniProtKB: Q9HA923   

Export aliases for RSAD1 gene to outside databases

Previous GC identifers: GC17P045912 GC17P048556


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: RSAD1_HUMAN, Q9HA92
Function: May be involved in porphyrin cofactor biosynthesis (By similarity)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the RSAD1 gene promoter:
         FOXO1a   COUP-TF1   HNF-4alpha2   FOXO4   FAC1   PPAR-gamma2   PPAR-gamma1   FOXO3b   FOXO3a   GR-alpha   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for RSAD1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for RSAD1 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q21.33   Ensembl cytogenetic band:  17q21.33   HGNC cytogenetic band: 17q21.33

RSAD1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
RSAD1 gene location

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P043924:  view genomic region     (about GC identifiers)

Start:
43,924,104 bp from pter
End:
43,931,255 bp from pter
Size:
7,152 bases
Orientation:
plus strand

1 alternative location:
Chr17+ 48,556,161-48,563,336     
RefSeq DNA sequence:
NC_000017.10  NT_010783.15  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: RSAD1_HUMAN, Q9HA92 (See protein sequence)
Recommended Name: Radical S-adenosyl methionine domain-containing protein 1, mitochondrial precursor  
Size: 442 amino acids; 48714 Da
Cofactor: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable
S-adenosyl-L-methionine (Potential)
Subcellular location: Mitochondrion (Potential)
Secondary accessions: Q53HV8 Q86VC4 Q9BRY7 Q9NUS7

Explore the universe of human proteins at neXtProt for RSAD1: NX_Q9HA92 

REFSEQ proteins: NP_060816.1  

ENSEMBL proteins: 
ENSP00000258955 ENSP00000424558 ENSP00000425372 ENSP00000430352 ENSP00000422893 ENSP00000395071 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
Browse OriGene Protein Over-expression Lysates
GenScript Custom Purified and Recombinant Proteins Services for RSAD1 
Novus Biologicals Protein for RSAD1
Browse Sino Biological Recombinant Proteins
Browse ProSpec Recombinant Proteins

2 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
About this table

RSAD1 for ontologies           About GeneDecksing



Antibodies for RSAD1: 
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Sigma-Aldrich Antibodies for RSAD1
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Browse antibodies at Epitomics

Assays for RSAD1: 
Browse Kits and Assays available from Millipore
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OriGene Custom Immunoassay Development 
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Browse R&D Systems for biochemical assays
GenScript Custom Assay Services for RSAD1 
Browse Enzo Life Sciences for kits & assays
Uscn ELISAs and CLIAs for RSAD1 


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

RSAD1 for domains           About GeneDecksing

4 InterPro domains/families:
 IPR010723 HemN_C
 IPR007197 Radical_SAM
 IPR006638 Elp3/MiaB/NifB
 IPR004559 HemN-rel

Graphical View of Domain Structure for InterPro Entry Q9HA92

ProtoNet protein and cluster: Q9HA92

1 Blocks protein family: IPB006638 Elongator protein 3/MiaB/NifB

UniProtKB/Swiss-Prot: RSAD1_HUMAN, Q9HA92
Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family


(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: RSAD1_HUMAN, Q9HA92
Function: May be involved in porphyrin cofactor biosynthesis (By similarity)
Enzyme Number (IUBMB): EC 1.3.99.- 

Inhib.
RNA:
    
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for RSAD1
Sigma-Aldrich shRNA for RSAD1
OriGene 29mer shRNA kits in GFP-retroviral vector: RSAD1
OriGene shRNA RFP: RSAD1
OriGene basic RS shRNA: RSAD1
OriGene siRNA: RSAD1
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of RSAD1 
miRNA:Sigma-Aldrich microRNA Mimics for RSAD1
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of RSAD1 
1 SABiosciences Assays for microRNA that regulate RSAD1:
hsa-miR-331-3p

Gene
Editing:
Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: RSAD1
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: RSAD1
OriGene untagged cDNA clones in CMV expression vector: RSAD1
OriGene 3'-UTR clone: RSAD1 
Browse MicroRNA Expression Plasmids 
GenScript cDNA clone with any tag delivered in your preferred vector: RSAD1 
Browse Sino Biological Human cDNA Clones

Cell
Lines:
  
GenScript Custom overexpressing Cell Line Services for RSAD1 

5/6 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 6):

GO IDQualified GO termEvidencePubMed IDs
GO:0003824 catalytic activity ----
GO:0004109 coproporphyrinogen oxidase activity IEA--
GO:0016491 oxidoreductase activity IEA--
GO:0046872 metal ion binding IEA--
GO:0051536 iron-sulfur cluster binding ----
About this table

RSAD1 for ontologies           About GeneDecksing


(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

    SABiosciences Custom Pathway-Focused PCR Arrays for RSAD1 

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for RSAD1

2 Gene Ontology (GO) biological process terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006779 porphyrin biosynthetic process IEA--
GO:0055114 oxidation-reduction process IEA--
About this table

RSAD1 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for RSAD1


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib.
RNA:
     
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for RSAD1
Sigma-Aldrich shRNA for RSAD1
OriGene 29mer shRNA kits in GFP-retroviral vector: RSAD1
OriGene shRNA RFP: RSAD1
OriGene basic RS shRNA: RSAD1
OriGene siRNA: RSAD1
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of RSAD1 
miRNA: Sigma-Aldrich microRNA Mimics for RSAD1
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of RSAD1 
1 SABiosciences Assays for microRNA that regulate RSAD1:
hsa-miR-331-3p
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: RSAD1
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: RSAD1
OriGene untagged cDNA clones in CMV expression vector: RSAD1
OriGene 3'-UTR Clone: RSAD1 
Browse OriGene MicroRNA Expression Plasmids 
GenScript cDNA clone with any tag delivered in your preferred vector: RSAD1 
Primers: OriGene genome-wide validated SYBR primer pairs: RSAD1
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for RSAD1: PPH07581A

REFSEQ mRNAs for RSAD1 gene: 

NM_018346.1  

Additional cDNA sequence: 

AK002026.1 AK022105.1 AK024628.1 AK222472.1 AK293814.1 AK297655.1 BC005854.2 BC050538.1 
CR621437.1 

14 DOTS entries:

DT.442467  DT.100792965  DT.100792967  DT.402970  DT.100792963  DT.120971878  DT.75160905  DT.95273663 
DT.120971901  DT.95087348  DT.100659056  DT.100792962  DT.120971896  DT.86855383 

24/233 AceView cDNA sequences (see all 233):

AV653956 CD513517 BM454672 BM713111 BM677032 AK024628 BM916831 CB140338 
BF805228 BI822661 BC050538 BQ066444 NM_018346 BQ065969 BE671496 BM701378 
BF805231 BQ012945 BQ064844 CR621437 BQ059574 AA437121 BM561668 BQ437032 

highest scoring ESTs for RSAD1:

N94998 AA055526 AA055527 AA171610 AA171769 AA302984 AA303758 AA350538 AA351438 AA361061 

Unigene Cluster for RSAD1:

Radical S-adenosyl methionine domain containing 1
Hs.8033  [show with all ESTs]
Unigene Representative Sequence: AK002026


GeneLoc Exon Structure

10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000258955(uc002iqw.1 uc010wmq.1) ENST00000443328(uc010wmp.1)
ENST00000515221 ENST00000504284 ENST00000510554 ENST00000506211 ENST00000509398
ENST00000513650 ENST00000511005 ENST00000451010

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

RSAD1 expression in normal and diseased human tissues

1  / 2  / 3

3 probe-sets matching RSAD1 gene

Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
55024_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 N94998 1.00 1.00 1.00 1
218307_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018346 0.60 1.00 0.82 1
218307_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table

RSAD1 for expression           About GeneDecksing

Data from Genenote  (Publications) and GNF BioGPS
    About these images
RSAD1 gene expression
RSAD1 gene electronic northern expression
RSAD1 gene sage expression
About these images

CGAP SAGE TAG: GTTCTATTTA

SOURCE GeneReport for Unigene cluster: Hs.8033
Primers: OriGene genome-wide validated SYBR primer pairs: RSAD1
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for RSAD1: PPH07581A
    SABiosciences Custom PCR Arrays for RSAD1 


(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for RSAD1 gene from 5/6 species (see all 6)
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
RSAD11   -- radical S-adenosyl methionine domain containing 1 83.53(n)
82.83(a)
609330  XM_846575.1  XP_851668.1 
chimpanzee
(Pan troglodytes)
RSAD11   -- radical S-adenosyl methionine domain containing 1 99.32(n)
98.64(a)
455175  XM_511926.2  XP_511926.1 
cow
(Bos taurus)
RSAD11   -- radical S-adenosyl methionine domain containing 1 86.95(n)
86.43(a)
615755  XM_867904.2  XP_872997.2 
mouse
(Mus musculus)
Rsad11 , 5 11 (58.90 cM)5
radical S-adenosyl methionine domain containing 11, 5 83.79(n)1
84.62(a)1
2379261  NM_001013381.11  NP_001013399.11 
 AA5373595  AK0467065  (see all 13)
zebrafish
(Danio rerio)
rsad11   -- radical S-adenosyl methionine domain containing 1 59.07(n)
60.51(a)
565897  NM_001083557.1  NP_001077026.1 
About this table        Species with no ortholog for RSAD1

ENSEMBL Gene Tree for RSAD1 (if available)

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
  --

(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

10/80 NCBI SNPs in RSAD1 are shown (see all 80)
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidChr 17 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
----------
rs5571,2
C,F,O,A,48563234(-) TTCCCG/AAATTC 1 -- ut3114Minor allele frequency- A:0.16MN EA NS NA WA 2520
rs97751,2
C,F,48563289(-) TGTGAC/TCATTT 1 -- ut316Minor allele frequency- T:0.04MN NS EA WA 654
rs10439991,2
--48563146(+) CTCTTC/TTGAGC 1 -- ut31 ese30--------
rs10440251,2
--48563216(+) GACCAA/GAGAAA 1 -- ut31 ese3 trp31Minor allele frequency- G:0.00MN 184
rs10440351,2
--48563261(+) TAATTC/TTTCTT 1 -- ut311Minor allele frequency- T:0.00MN 184
rs11342931,2
--48560556(+) aaaaaA/Gaaaaa 1 -- int10--------
rs15517171,2
C,F,A,48560883(-) GGGGAT/CCCTGG 1 -- int18Minor allele frequency- C:0.14NS EA NA WA 1364
rs30630251,2
C48560553(+) aaaaaAAAG    
   AAA
/-
gaaag
1 -- int1 trp31Minor allele frequency- -:0.00NA 2
rs31808931,2
--48560562(+) agaaaA/Gaaaga 1 -- int10--------
rs32177161,2
C48556237(-) GCCCA-/GCCCA 
        
GCGCA
1 -- ut510--------
About this table

HapMap Linkage Disequilibrium images for RSAD1 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
      Database of Genomic Variants (DGV) variations for RSAD1: --

QIAGEN SeqTarget long-range PCR primers for resequencing RSAD1 

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

RSAD1 for disorders           About GeneDecksing

Human Genome Epidemiology (HuGE) Navigator: RSAD1 (1 document)

Export disorders and mutations for RSAD1 gene to outside databases

(Possibly Related Articles in Doctor's Guide)
About This Section
  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

6 PubMed articles for RSAD1 gene integrated from 7 sources:
(articles sorted by number of sources associating them with RSAD1)
  1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
  2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (2002)
  3. Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1 Hendrickson S.L....O'Brien S.J. (2010)
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
  5. Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)1 Suzuki Y.... Sugano S. (1997)
  6. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)1 Maruyama K. and Sugano S. (1994)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 55316 HGNC: 25634 AceView: FLJ11164 Ensembl:ENSG00000136444 euGenes: HUgn55316
ECgene: RSAD1 H-InvDB: RSAD1

(According to HUGE)
About This Section
  --

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
  --

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for RSAD1 gene:
Search GeneIP for patents involving RSAD1

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

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 "Your Favorite Gene" Pathways and Molecular Interaction Networks Browse Nano Scale siRNA at Sigma-Aldrich
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 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of RSAD1
 SeqTarget long-range PCR primers for resequencing RSAD1
 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for RSAD1
 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of RSAD1
 GenScript Custom Purified and Recombinant Proteins Services for RSAD1 GenScript cDNA clones with any tag delivered in your preferred vector for RSAD1
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 ELISAs and CLIAs for RSAD1
       

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