Aliases &
Descriptions
for SPHK2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| sphingosine kinase 21 2 | | SPK 22 3 | | SK 22 3 | | sphingosine kinase type 2 isoform2 | | EC 2.7.1.913 |
Export aliases for SPHK2 gene to outside databasesPrevious GC identifers: GC19P049767 GC19P049490 GC19P053798 GC19P053814 GC19P049122 |
Summaries
for SPHK2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator withboth intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine andL-threo-dihydrosphingosine. Binds phosphoinositides
|
Genomic Views
for SPHK2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the SPHK2 gene promoter:
AP-1 c-Jun c-Fos COUP-TF1 HNF-4alpha2 p53 STAT5A PPAR-gamma2 PPAR-gamma1 c-Myc
Other transcription factors
Search SABiosciences Chromatin IP Primers for SPHK2
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for SPHK2  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19q13.2 Ensembl cytogenetic band: 19q13.33 HGNC cytogenetic band: 19q13.2SPHK2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19P045501: view genomic region
(about GC identifiers)
Start:
|
45,501,739 bp from pter |
End:
|
45,513,198 bp from pter |
Size:
|
11,460 bases |
Orientation:
|
plus strand |
1 alternative location:
| Chr19+ 49,122,548-49,133,973 |
|
Proteins
for SPHK2 gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0 (See
protein sequence)Recommended Name: Sphingosine kinase 2 Size: 654 amino acids; 69217 Da
Cofactor: Magnesium (By similarity)
Subcellular location: Isoform 1: Cytoplasm. Membrane
Subcellular location: Isoform 2: Lysosome membrane
Secondary accessions: Q9BRN1 Q9H0Q2 Q9NWU7Alternative splicing: 3 isoforms: Q9NRA0-1 Q9NRA0-2 Q9NRA0-3 (Experimental confirmation may be lacking for some isoforms)Explore the universe of human proteins at neXtProt for SPHK2: NX_Q9NRA0
Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_064511.2
ENSEMBL proteins: ENSP00000385562 ENSP00000341091 ENSP00000245222 ENSP00000413369 ENSP00000410044
Human Recombinant Proteins
5 Gene Ontology (GO) cellular component terms (GO ID links to tree view): About this table
SPHK2 for ontologies About GeneDecksing
Antibodies for SPHK2:
Assays for SPHK2: |
Protein
Domains/
Families
for SPHK2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
SPHK2 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q9NRA0ProtoNet protein and cluster: Q9NRA0 UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0Similarity: Contains 1 DAGKc domain |
Gene Function
for SPHK2 gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: SPHK2_HUMAN, Q9NRA0Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator withboth intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine andL-threo-dihydrosphingosine. Binds phosphoinositidesCatalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphateCatalytic activity: ATP + sphingosine = ADP + sphingosine 1-phosphateEnzyme regulation: Inhibited by sulfatideBiophysicochemical properties: Kinetic parameters: KM=3.4 uM for sphingosine; pH dependence: Optimum pH is 7.5;Enzyme Number (IUBMB): EC 2.7.1.91
5/9 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 9): About this table
SPHK2 for ontologies About GeneDecksing
Animal Models: 9 MGI mutant phenotypes (inferred from 7 alleles ) (MGI details for Sphk2):
SPHK2 for phenotypes About GeneDecksing
|
Pathways & Interactions
for SPHK2 gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
SPHK2 for pathways About GeneDecksing
5/6  Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathways for SPHK2 (see all 6)
5  Kegg Pathways (Kegg details for SPHK2):
SABiosciences Pathway-Focused PCR Arrays including SPHK2: PAHS-037A PAHS-091A
Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for SPHK2
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for SPHK2
5/26 Interacting proteins for SPHK2 (Q9NRA01 ENSP000002452223) via UniProtKB, MINT, and/or STRING (see all 26)About this table
5/9 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 9): About this table
SPHK2 for ontologies About GeneDecksing
|
Drugs & Compounds
for SPHK2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
SPHK2 for compounds About GeneDecksing
 Compounds for SPHK2 available from Tocris Bioscience
| Compound | Action |
CAS
number |
|---|
| SKI II | Selective non-lipid inhibitor of sphingosine kinase; antitumor | [312636-16-1] |
About this table
2  Novoseek chemical compound relationships for SPHK2 gene
About this table
|
Transcripts
for SPHK2 gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for SPHK2 gene: NM_020126.3 11 AceView cDNA sequences: BC006161 NM_020126 AK122735 AL136701 CR614720 BC010671 AK000599 AF245447 AB100373 AK074601 CO246179
 highest scoring ESTs for SPHK2:AB100373 AL580072 BM793811 AA907391 AK125560 AL583449 AW196191 BG171363 BM803297 BQ337947
GeneLoc Exon Structure
5/20 Alternative Splicing Database (ASD) splice patterns (SP) for SPHK2 (see all 20)
| ExUns: | 1 | ^ | 2a | · | 2b | · | 2c | · | 2d | · | 2e | ^ | 3a | · | 3b | ^ | 4 | ^ | 5a | · | 5b | · | 5c | ^ | 6 | ^ | 7a | · | 7b | · | 7c | ^ | 8 | ^ | 9 | ^ | 10a | · | 10b | · | 10c | ^ | 11 | ^ | 12 | ^ | 13a | · | 13b | · | 13c | ^ |
|---|
|
| SP1: | | | | | | | | | | | | | - | | - | | - | | | | | | - | | - | | - | | - | | - | | - | | | | | | - | | - | | | | | | - | | | | | | |
| SP2: | | | | | | | | | | | | | - | | - | | - | | | | | | - | | - | | - | | - | | - | | - | | | | | | | | | | | | | | - | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | |
| SP4: | | | - | | - | | - | | - | | - | | - | | - | | - | | | | | | - | | - | | - | | - | | - | | - | | | | | | | | | | | | | | - | | | | | | |
| SP5: | | | | | | | | | | | | | - | | - | | - | | | | | | - | | - | | - | | - | | - | | - | | - | | - | | - | | - | | - | | - | | - | | | | | |
| ExUns: | 14 | ^ | 15a | · | 15b | · | 15c | · | 15d | ^ | 16a | · | 16b | ^ | 17a | · | 17b | ^ | 18 |
|---|
|
| SP1: | | | | | | | | | - | | | | | | | | | | | | |
| SP2: | | | | | | | | | - | | | | | | | | | | | | |
| SP3: | | | | | | | | | - | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | - | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for SPHK2
5 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000406269(uc002pjt.2) ENST00000340932(uc010xzu.1) ENST00000245222(uc002pjr.2 uc010xzt.1 uc002pjs.2 uc002pjv.2)
ENST00000443164(uc002pju.2 uc002pjw.2) ENST00000426514
|
Expression
for SPHK2 gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| SPHK2 expression in normal and diseased human tissues
1 / 2 / 3 6 probe-sets matching SPHK2 gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 40273_at2, 3
|
U133-A |
1 |
0.94 |
1.00 |
0.82 |
1.03 |
AA485440 |
0.60 |
0.75 |
0.68 |
1 |
| 88521_at2, 3
|
U95-E |
1 |
0.38 |
1.00 |
0.78 |
0.97 |
AA702497 |
0.20 |
0.50 |
0.38 |
1 |
| 209857_s_at2, 3
|
U133-A |
1 |
1.00 |
1.00 |
-- |
-- |
AF245447 |
0.60 |
0.75 |
0.68 |
1 |
| 40273_at2, 3
|
U133-A |
1 |
0.94 |
1.00 |
-- |
-- |
AA485440 |
0.60 |
0.75 |
0.68 |
1 |
| 209857_s_at2
|
U133Plus2 |
1 |
1.00 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 40273_at2
|
U133Plus2 |
1 |
0.94 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
SPHK2 for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: --
Expression variation in blood from EXPOLDB for SPHK2 SABiosciences Expression via Pathway-Focused PCR Arrays including SPHK2: PAHS-037A PAHS-091A
|
Orthologs
for SPHK2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for SPHK2 gene from 5/9 species (see all 9)
About this table Species with no ortholog for SPHK2
ENSEMBL Gene Tree for SPHK2 (if available) |
Paralogs
for SPHK2 gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for SPHK2 gene
- SPHK12 AGK2
SPHK2 for paralogs About GeneDecksing
|
Genomic Variants
for SPHK2 gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 19 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for SPHK2 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for SPHK2
1 CNV: 32261
1 Indel: 40287 | | QIAGEN SeqTarget long-range PCR primers for resequencing SPHK2 |
|
Disorders & Mutations
for SPHK2 gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
SPHK2 for disorders About GeneDecksing
OMIM: 607092
1 Novoseek disease relationship for SPHK2 gene
About this table
Human Genome Epidemiology (HuGE) Navigator: SPHK2 (1 document)
Export disorders and mutations for SPHK2 gene to outside databases
|
Medical News
for SPHK2 gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for SPHK2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/63 PubMed articles for SPHK2 gene, integrated from 7 sources (see all 63):
(articles sorted by number of sources associating them with SPHK2)- Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. (PubMed id 10751414)1, 2, 3, 7 Liu H.... Spiegel S. (2000)
- A lipid binding domain in sphingosine kinase 2. (PubMed id 19168031)1, 2, 7 Don A.S. and Rosen H. (2009)
- Recycling of sphingosine is regulated by the concerte d actions of sphingosine-1-phosphate phosphohydrolase 1 and sphingosine kinase 2. (PubMed id 17895250)1, 3 Le Stunff H....Spiegel S. (2007)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (2001)
- Role of sphingosine kinase 2 in cell migration toward epidermal growth factor. (PubMed id 15951439)1, 7 Hait N.C....Spiegel S. (2005)
- Expression of sphingosine kinase 2 in synovial fibrob lasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analog ue, FTY720. (PubMed id 19490468)1, 7 Kamada K....Nose M. (2009)
- Protein kinase D-mediated phosphorylation and nuclear export of sphingosine kinase 2. (PubMed id 17635916)1, 7 Ding G....Nakamura S. (2007)
- Sphingosine kinase type 2 activation by ERK-mediated phosphorylation. (PubMed id 17311928)1, 7 Hait N.C....Spiegel S. (2007)
|
External Searches for SPHK2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing SPHK2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing SPHK2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing SPHK2 gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for SPHK2 gene:
Search GeneIP for patents involving SPHK2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for SPHK2 gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
|
 | |
 |
| | | |
 |
 |
| | |
 |  |  |  | |
| |  | Tocris compounds for SPHK2 |
| |  |  |  |  | | | | |
 | | | ELISAs and CLIAs for SPHK2 |
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