Aliases &
Descriptions
for STK3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases & Descriptions |
|---|
| serine/threonine kinase 31 2 | | MST-22 3 | | MST21 2 3 | | STE20-like kinase MST22 3 | | serine/threonine kinase 3 (Ste20, yeast homolog)1 2 | | serine/threonine kinase 3 (STE20 homolog, yeast)2 | | KRS11 2 | | FLJ907482 | | Serine/threonine-protein kinase Krs-12 3 | | serine/threonine-protein kinase 32 | | Mammalian STE20-like protein kinase 22 3 | | EC 2.7.11.13 |
Export aliases for STK3 gene to outside databasesPrevious GC identifers: GC08M098475 GC08M099540 GC08M099135 GC08M099423 GC08M099536 GC08M099466 |
Summaries
for STK3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for STK3:
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, orapoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmentalconditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascadethat is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by theproapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 (PubMed 8816758); Lee et al., 2001(PubMed 11278283)).(supplied by OMIM)
UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induceschromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathwaywhich plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promotingapoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatoryprotein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turnphosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits itstranslocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cellmigration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultativeadult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity)
Gene Wiki entry for STK3 |
Genomic Views
for STK3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 60),
Regulatory elements and Epigenetics data according to
Qiagen and/or
SABiosciences)
About This Section
|
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the STK3 gene promoter:
ITF-2 Tal-1beta Meis-1 E47 Tal-1 GR-beta GR-alpha TGIF AhR FOXL1
Other transcription factors
Search SABiosciences Chromatin IP Primers for STK3
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for STK3  |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 8q22.2 Ensembl cytogenetic band: 8q22.2 HGNC cytogenetic band: 8q22.2STK3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 8 GeneLoc Exon Structure GeneLoc location for GC08M094670: view genomic region
(about GC identifiers)
Start:
|
94,670,088 bp from pter |
End:
|
95,040,917 bp from pter |
Size:
|
370,830 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr8- 99,413,631-99,955,055 |
RefSeq DNA sequence:- NC_000008.10 NT_008046.16
|
Proteins
for STK3 gene
(According to
1UniProtKB,
neXtProt,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological, and/or
ProSpec,
Biochemical Assays by
Millipore,
Sigma-Aldrich,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Dec 2010 and
Entrez Gene,
Antibodies by
Millipore,
Sigma-Aldrich,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals, and/or
Epitomics)
About This Section
|
UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188 (See
protein sequence)Recommended Name: Serine/threonine-protein kinase 3 Size: 491 amino acids; 56301 Da
Cofactor: Magnesium
Subunit: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (Bysimilarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation.Interacts with RASSF1, which leads to enzyme activation
Subcellular location: Cytoplasm (By similarity). Nucleus (By similarity). Note=The caspase-cleaved form cycles betweennucleus and cytoplasm (By similarity)
Secondary accessions: A8K722 Q15445 Q15801 Q96FM6Explore the universe of human proteins at neXtProt for STK3: NX_Q13188
Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_006272.2
ENSEMBL proteins: ENSP00000341976 ENSP00000347009 ENSP00000428295 ENSP00000428167 ENSP00000390500 ENSP00000428014 ENSP00000429369 ENSP00000429744
Human Recombinant Proteins
2 Gene Ontology (GO) cellular component terms (GO ID links to tree view): About this table
STK3 for ontologies About GeneDecksing
Antibodies for STK3:
Assays for STK3: |
Protein
Domains/
Families
for STK3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
STK3 for domains About GeneDecksing
5/6 InterPro domains/families (see all 6):Graphical View of Domain Structure for InterPro Entry Q13188ProtoNet protein and cluster: Q13188 UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamilySimilarity: Contains 1 protein kinase domainSimilarity: Contains 1 SARAH domain |
Gene Function
for STK3 gene
(According to UniProtKB,
IUBMB,and/or
Genatlas, Animal models from MGI Dec 24 2010,
shRNA from
OriGene,
Sigma-Aldrich,
RNAi from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Clones from Millipore,
Sigma-Aldrich,
OriGene,
GenScript,
Sino Biological,
Cell Lines from GenScript,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene.)
About This Section
|
UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188Function: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induceschromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathwaywhich plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promotingapoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatoryprotein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turnphosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits itstranslocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cellmigration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultativeadult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity)Catalytic activity: ATP + a protein = ADP + a phosphoproteinEnzyme regulation: Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activationalso requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene productRAF1Enzyme Number (IUBMB): EC 2.7.11.1
Genatlas biochemistry entry for STK3:serine threonine kinase 35/9 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 9): About this table
STK3 for ontologies About GeneDecksing
Animal Models: 15/17 MGI mutant phenotypes (inferred from 6 alleles ) (MGI details for Stk3) (see all 17):
STK3 for phenotypes About GeneDecksing
|
Pathways & Interactions
for STK3 gene
(Pathways according to
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
PCR Arrays from
SABiosciences,
Proteins Network according to
SABiosciences,
Sigma-Aldrich,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Dec 2010 via
Entrez Gene).
About This Section
|
STK3 for pathways About GeneDecksing
1  Kegg Pathway (Kegg details for STK3):
SABiosciences Custom Pathway-Focused PCR Arrays for STK3
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for STK3
3 Interacting proteins for STK3 (Q131881 ENSP000003470093) via UniProtKB, MINT, and/or STRINGAbout this table
5/6 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 6): About this table
STK3 for ontologies About GeneDecksing
|
Drugs & Compounds
for STK3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
STK3 for compounds About GeneDecksing
 Browse Tocris compounds for STK3
2  Novoseek chemical compound relationships for STK3 gene
About this table
|
Transcripts
for STK3 gene(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
Millipore,
siRNAs from
Sigma-Aldrich,
OriGene,
Qiagen,
Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
microRNA from Sigma-Aldrich,
Qiagen,
SABiosciences,
Tagged/untagged cDNA clones from
OriGene,
Sigma-Aldrich,
GenScript,
Primers from
OriGene and/or
SABiosciences)
About This Section
|
REFSEQ mRNAs for STK3 gene: NM_006281.2 Additional cDNA sequence: AK075229.1 AK131363.1 AK291837.1 AK307787.1 AK312660.1 BC010640.2 CR627416.1 U26424.1 U60206.1 Z25422.1 11 DOTS entries: DT.215842 DT.100637314 DT.91721027 DT.121466825 DT.100754405 DT.102833033 DT.121466843 DT.86858028 DT.91875264 DT.92014795 DT.97793520 24/141 AceView cDNA sequences (see all 141): BM994152 BC010640 AA370618 R22038 CD742983 AI284049 AL708786 BQ423964 AA464529 AL527704 AA371498 BP335254 AA954682 BI964879 BU689201 AW300076 CB143850 AL546605 AK131363 AW168289 BQ723525 AL079539 BU182450 BF035235
 highest scoring ESTs for STK3:U26424 AL546605 AL702996 AU125963 AW408138 BG704885 BI544404 BI545659 BI553518 BQ362641 Unigene Cluster for STK3: Serine/threonine kinase 3 Hs.492333 [show with all ESTs]Unigene Representative Sequence: CR627416
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for STK3
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9 | ^ | 10 | ^ | 11 |
|---|
|
| SP1: | | | | | | | | | | | - | | | | - | | - | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | - | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for STK3
18/19 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19): ENST00000523159 ENST00000339879 ENST00000354930(uc003yio.2) ENST00000519420
ENST00000520053 ENST00000523960 ENST00000424861(uc004emd.2) ENST00000518582
ENST00000520653 ENST00000419617(uc003yip.2 uc010mbm.1) ENST00000522924
ENST00000518165 ENST00000520440 ENST00000517832 ENST00000523567 ENST00000521649
ENST00000521768 ENST00000518627
|
Expression
for STK3 gene
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 228 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Primers from
OriGene and/or
SABiosciences
)
About This Section
| STK3 expression in normal and diseased human tissues
1 / 2 / 3 5 probe-sets matching STK3 gene
Affymetrix
probe-set |
Array |
GeneAnnot data |
GeneNote data |
GeneTide data |
| # genes |
Sensitivity |
Specificity |
Correlation |
Length |
Gb_Accession |
Consensus |
Uniqueness |
Score |
Rank |
| 32142_at2, 3
|
U95-A |
1 |
0.81 |
1.00 |
1.00 |
1.00 |
U26424 |
1.00 |
0.90 |
0.95 |
1 |
| 211078_s_at2, 3
|
U133-A |
1 |
0.82 |
1.00 |
-- |
-- |
Z25422 |
0.40 |
1.00 |
0.76 |
1 |
| 204068_at2, 3
|
U133-A |
1 |
0.73 |
1.00 |
-- |
-- |
NM_006281 |
0.60 |
1.00 |
0.82 |
1 |
| 211078_s_at2
|
U133Plus2 |
1 |
0.82 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
| 204068_at2
|
U133Plus2 |
1 |
0.73 |
1.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
About this table
STK3 for expression About GeneDecksing
Data from
Genenote
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: TTCCTGATGA
SOURCE GeneReport for Unigene cluster: Hs.492333
Expression variation in blood from EXPOLDB for STK3 UniProtKB/Swiss-Prot: STK3_HUMAN, Q13188Tissue specificity: Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels inadult heart, lung and brain tissues
SABiosciences Custom PCR Arrays for STK3
|
Orthologs
for STK3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Dec 24 2010,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for STK3 gene from 5/10 species (see all 10)
About this table Species with no ortholog for STK3
ENSEMBL Gene Tree for STK3 (if available) |
Paralogs
for STK3 gene(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for STK3 gene
- STK242 STK42 STK252 MST42
STK3 for paralogs About GeneDecksing
|
Genomic Variants
for STK3 gene(SNPs according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Chr 8 pos | Sequence | Recs | AA
Chg | Type | More | Recs | Allele
freq | Pop | Total
sample | More |
|---|
About this tableHapMap Linkage Disequilibrium images for STK3 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for STK3
1 CNV: 100435 | | QIAGEN SeqTarget long-range PCR primers for resequencing STK3 |
|
Disorders & Mutations
for STK3 gene
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
LSDB, HGMD, GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
STK3 for disorders About GeneDecksing
OMIM: 605030
2 Novoseek disease relationships for STK3 gene
About this table
Export disorders and mutations for STK3 gene to outside databases
|
Medical News
for STK3 gene(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for STK3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6UniProtKB/TrEMBL, and/or
7Novoseek)
About This Section
|
10/45 PubMed articles for STK3 gene, integrated from 7 sources (see all 45):
(articles sorted by number of sources associating them with STK3)- Newly identified stress-responsive protein kinases, Krs-1 and Krs- 2. (PubMed id 8816758)1, 2, 3 Taylor L.K.... Erikson R.L. (1996)
- Role of the kinase MST2 in suppression of apoptosis by the proto- oncogene product Raf-1. (PubMed id 15618521)1, 2, 7 O'Neill E.... Kolch W. (2004)
- Cloning and characterization of a member of the MST subfamily of Ste20-like kinases. (PubMed id 8566796)1, 2, 7 Creasy C.L. and Chernoff J. (1995)
- The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. (PubMed id 15688006)1, 2, 7 Chan E.H.Y.... Sillje H.H.W. (2005)
- Role of the tumor suppressor RASSF1A in Mst1-mediated apoptosis. (PubMed id 16510573)1, 2 Oh H.J.... Lim D.-S. (2006)
- Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation. (PubMed id 16930133)1, 2 Callus B.A.... Vaux D.L. (2006)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. (PubMed id 8274451)1, 2 Schultz S.J. and Nigg E.A. (1993)
- Mammalian Ste20-like kinase (Mst2) indirectly support s Raf-1/ERK pathway activity via maintenance of protein phosphatase-2A catalyti c subunit levels and consequent suppression of inhibitory Raf-1 phosphorylation . (PubMed id 20212043)1, 7 Kilili G.K. and Kyriakis J.M. (2010)
|
External Searches for STK3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing STK3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing STK3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing STK3 gene(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for STK3 gene:
Search GeneIP for patents involving STK3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for STK3 gene(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or
Enzo Life Sciences)
About This Section
|
 | |
 |
| | | |
 |
 |
| | |
 |  |  |  | |
 | Antibodies & Assays for STK3 (MST2) |
| | | | Search Tocris compounds for STK3 |
| |  |  |  |  | | | | |
 | | | ELISAs and CLIAs for STK3 |
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