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WDR65 Gene

protein-coding   GIFtS: 32

GC01P041757
WD repeat domain 65
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Products    

Aliases &
Descriptions
for WDR65 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases & Descriptions
WD repeat domain 651 2
FLJ320001 2
WD repeat-containing protein 652

External Ids:    HGNC: 264851   Entrez Gene: 1494652   Ensembl: ENSG000002437107   UniProtKB: Q96MR63   

Export aliases for WDR65 gene to outside databases

Previous GC identifers: GC01P043309 GC01P043410 GC01P043638


Summaries
for WDR65 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
  --

Genomic Views
for WDR65 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the WDR65 gene promoter:
         PPAR-gamma2   PPAR-gamma1   E2F   E2F-1   YY1   Elk-1   ARP-1   USF1   STAT3   RFX1   
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for WDR65

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for WDR65 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p34.2   Ensembl cytogenetic band:  1p34.2   HGNC cytogenetic band: 1p34.2

WDR65 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WDR65 gene location

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P041757:  view genomic region     (about GC identifiers)

Start:
 41,757,760 bp from pter
End:
 41,796,304 bp from pter
Size:
 38,545 bases
Orientation:
 plus strand

1 alternative location:
Chr1+ 43,638,026-43,676,549     
RefSeq DNA sequence:
NC_000001.10  NT_032977.9  

Proteins
for WDR65 gene

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section
UniProtKB/Swiss-Prot: WDR65_HUMAN, Q96MR6 (See protein sequence)
Recommended Name: WD repeat-containing protein 65  
Size: 1250 amino acids; 144961 Da
Secondary accessions: A6NKQ3 Q17RI9 Q5TAI0
Alternative splicing: 2 isoforms:  Q96MR6-1   Q96MR6-2   

Explore the universe of human proteins at neXtProt for WDR65: NX_Q96MR6 

REFSEQ proteins (4 alternative transcripts): 
NP_001161437.1  NP_001161438.1  NP_001182760.1  NP_689711.2  


ENSEMBL proteins: 
ENSP00000296394 


Human Recombinant Proteins 
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OriGene Protein Over-expression Lysate: WDR65
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Antibodies for WDR65: 
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Assays for WDR65: 
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OriGene Custom Immunoassay Development 
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GenScript Custom Assay Services for WDR65 
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Browse ELISAs and CLIAs at Uscn 

Protein Domains/
Families
for WDR65 gene

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
WDR65 for domains           About GeneDecksing

5/8 InterPro domains/families (see all 8):
 IPR019782 WD40_repeat_2
 IPR011046 WD40_repeat-like_dom
 IPR019781 WD40_repeat_sg
 IPR011047 Quino_AlcDH-like
 IPR009053 Prefoldin

Graphical View of Domain Structure for InterPro Entry Q96MR6

ProtoNet protein and cluster: Q96MR6

UniProtKB/Swiss-Prot: WDR65_HUMAN, Q96MR6
Similarity: Contains 10 WD repeats


Gene Function
for WDR65 gene

(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section
Inhib.
RNA:    		Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for WDR65
Sigma-Aldrich shRNA for WDR65
OriGene 29mer shRNA kits in GFP-retroviral vector (see all 4): WDR65
OriGene shRNA RFP (see all 4): WDR65
OriGene basic RS shRNA (see all 4): WDR65
OriGene siRNA (see all 4): WDR65
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of WDR65 
miRNA:Browse microRNA Mimics at Sigma-Aldrich
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of WDR65 
Search SABiosciences for Assays for microRNAs that regulate WDR65

Gene
Editing:		Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): WDR65
OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): WDR65
OriGene untagged cDNA clones in CMV expression vector: WDR65
OriGene 3'-UTR clone: WDR65 
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Cell
Lines:  		GenScript Custom overexpressing Cell Line Services for WDR65 


Pathways & Interactions
for WDR65 gene

(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

    SABiosciences Custom Pathway-Focused PCR Arrays for WDR65 

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for WDR65

Drugs & Compounds
for WDR65 gene

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for WDR65


Transcripts
for WDR65 gene

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section
Inhib.
RNA:     		Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for WDR65
Sigma-Aldrich shRNA for WDR65
OriGene 29mer shRNA kits in GFP-retroviral vector (see all 4): WDR65
OriGene shRNA RFP (see all 4): WDR65
OriGene basic RS shRNA (see all 4): WDR65
OriGene siRNA (see all 4): WDR65
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of WDR65 
miRNA: Browse microRNA Mimics at Sigma-Aldrich
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of WDR65 
Search SABiosciences for Assays for microRNAs that regulate WDR65
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): WDR65
OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): WDR65
OriGene untagged cDNA clones in CMV expression vector: WDR65
OriGene 3'-UTR Clone: WDR65 
Browse OriGene MicroRNA Expression Plasmids 
GenScript Custom cDNA clone Services for WDR65 
Primers: OriGene genome-wide validated SYBR primer pairs: WDR65
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for WDR65: PPH17971A

REFSEQ mRNAs for WDR65 gene (4 alternative transcripts): 

NM_001167965.1  NM_001167966.1  NM_001195831.1  NM_152498.3  

Additional cDNA sequence: 

AK056562.1 BC117280.1 BC117306.1 BC143804.1 CR936709.1 

10 DOTS entries:

DT.99938880  DT.105324  DT.121381894  DT.450421  DT.75147103  DT.121381838  DT.121381897  DT.91951808 
DT.100741532  DT.100649777 

5 AceView cDNA sequences:

NM_152498 BV190606 AA634516 CB157542 AK056562 

highest scoring ESTs for WDR65:

AK056562 AA634516 NM_152498 CB157542 DA817976 DB327723 AI076096 AI912957 AV659996 DA388943 

Unigene Cluster for WDR65:

WD repeat domain 65
Hs.527565  [show with all ESTs]
Unigene Representative Sequence: CR936709


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000296394(uc010ojz.1 uc001cip.1 uc001ciq.1)

Expression
for WDR65 gene

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

WDR65 expression in normal and diseased human tissues

1  / 2  / 3

1 probe-set matching WDR65 gene

Affymetrix
probe-set		 Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
1553425_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
About this table
Data from Genenote  (Publications) and GNF BioGPS
    About these images
WDR65 gene expression
WDR65 gene electronic northern expression
WDR65 gene sage expression
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.527565
Primers: OriGene genome-wide validated SYBR primer pairs: WDR65
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for WDR65: PPH17971A
    SABiosciences Custom PCR Arrays for WDR65 


Orthologs
for WDR65 gene

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section
Orthologs for WDR65 gene from 5/8 species (see all 8)
Organism Gene Locus Description Human
Similarity		 NCBI accessions
dog
(Canis familiaris)		 WDR651   -- WD repeat domain 65 88.89(n)
89.11(a)		 475390  XM_532614.2  XP_532614.2 
chimpanzee
(Pan troglodytes)		 WDR651   -- WD repeat domain 65 99.38(n)
99.32(a)		 469174  XM_524559.2  XP_524559.2 
cow
(Bos taurus)		 WDR651   -- WD repeat domain 65 88.77(n)
88.1(a)		 521560  XM_599825.3  XP_599825.3 
mouse
(Mus musculus)		 Wdr655 4 (54.84 cM) WD repeat domain 65   -- BC062805 
chicken
(Gallus gallus)		 WDR651   -- WD repeat domain 65 63.46(n)
56.52(a)		 424559  XM_422397.2  XP_422397.2 
About this table        Species with no ortholog for WDR65

ENSEMBL Gene Tree for WDR65 (if available)

Paralogs
for WDR65 gene

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
Paralogs for WDR65 gene
LOC1005067112  

WDR65 for paralogs           About GeneDecksing



Genomic Variants
for WDR65 gene

(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

10/380 NCBI SNPs in WDR65 are shown (see all 380)
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg		TypeMoreRecsAllele
freq		PopTotal
sample		More
----------
rs5130091,2
C,F,O,A,H,43663376(-) AAGCCC/TTTCAT 2 -- int120Minor allele frequency- T:0.25NA NS EA WA 1204
rs5869041,2
C,F,O,A,H,43645010(+) AGAGGC/TGAGGC 2 -- int17Minor allele frequency- T:0.18NA EA WA 298
rs5983361,2
C,F,A,H,43650741(-) AGAAGA/GCTGTC 2 -- int111Minor allele frequency- G:0.47NS EA NA WA 544
rs5987361,2
C,F,43663659(-) GGATAG/AGAGAG 2 -- int15Minor allele frequency- A:0.04NS EA WA 470
rs6026291,2
C,F,A,43670248(-) actgcT/Acacta 2 -- int110Minor allele frequency- A:0.27MN NS EA NA WA 810
rs6031231,2
C,F,O,A,43664218(-) ATGAAG/ATGTCC 6 /H ref1 syn113Minor allele frequency- A:0.20NS EA NA WA 692
rs6035601,2
C,F,O,A,43664119(-) TAAGCT/CTAGCT 2 -- int113Minor allele frequency- C:0.20NS EA NA WA 692
rs6053361,2
C,F,A,43669642(-) CCCACG/ATATGG 2 -- int19Minor allele frequency- A:0.30NS EA NA WA 634
rs6066551,2
--43644377(+) TTCAAC/GCATTG 2 -- int10--------
rs6129511,2
C,F,O,A,43664526(-) TAGTCT/GGGTGG 2 -- int112Minor allele frequency- G:0.18NS EA NA WA 556
About this table

HapMap Linkage Disequilibrium images for WDR65 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
      Database of Genomic Variants (DGV) variations for WDR65: --

QIAGEN SeqTarget long-range PCR primers for resequencing WDR65 

Disorders & Mutations
for WDR65 gene

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section
   --

Medical News
for WDR65 gene

(Possibly Related Articles in Doctor's Guide)
About This Section
   --

Publications
for WDR65 gene

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

9 PubMed articles for WDR65 gene integrated from 7 sources:
(articles sorted by number of sources associating them with WDR65)
  1. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
  4. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
  5. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
  6. Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein-protein interactions and complexes. (PubMed id 15867431)1 Miyamoto-Sato E....Yanagawa H. (2005)
  7. A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. (PubMed id 14743216)1 Bouwmeester T....Superti-Furga G. (2004)
  8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
  9. Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)1 Bonaldo M.F.... Soares M.B. (1996)

External Searches for WDR65 gene

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  
  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

Genome Databases showing WDR65 gene

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 149465 HGNC: 26485 AceView: FLJ32000 Ensembl:ENSG00000243710 euGenes: HUgn149465
ECgene: WDR65 H-InvDB: WDR65

Other Databases showing WDR65 gene

(According to HUGE)
About This Section
   --

Specialized Databases showing WDR65 gene

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
   --

Intellectual Property
for WDR65 gene

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for WDR65 gene:
Search GeneIP for patents involving WDR65

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



Products
for WDR65 gene

(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

 Millipore Custom Antibody & Bulk Services
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 miRNA Expression Plasmids

 		 
 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of WDR65
 SeqTarget long-range PCR primers for resequencing WDR65
 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for WDR65
 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of WDR65
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 Regulatory tfbs in WDR65 promoter
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