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ZAK Gene

protein-coding GIFtS: 51

GC02P165819

symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

		Products

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases & Descriptions
MRK² ³ ⁵		OTTHUMP00000205097²
Leucine zipper- and sterile alpha motif-containing kinase² ³		MLK7²
Human cervical cancer suppressor gene 4 protein² ³		mlklak²
Sterile alpha motif- and leucine zipper-containing kinase AZK² ³		pk²
MLK-like mitogen-activated protein triple kinase² ³		cervical cancer suppressor gene 4 protein²
HCCS-4² ³		mitogen-activated protein kinase kinase kinase MLT²
MLK-related kinase² ³		mixed lineage kinase 7²
MLTK² ⁵		protein kinase²
AZK²		mixed lineage kinase with a leucine zipper and a sterile alpha motif²
MLT²		EC 2.7.11.25³
sterile alpha motif and leucine zipper containing kinase AZK²		Mixed lineage kinase-related kinase³
mixed lineage kinase-related kinase MRK-beta²

External Ids:	Entrez Gene: 51776²	Ensembl: ENSG00000091436⁷	UniProtKB: Q9NYL2³

Export aliases for ZAK gene to outside databases

Previous GC identifers: GC02P171993 GC02P172601 GC02P173904 GC02P174143 GC02P173766 GC02P173648

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ZAK:

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal

kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding

protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to

cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein

also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been

characterized. (provided by RefSeq)

UniProtKB/Swiss-Prot: MLTK_HUMAN, Q9NYL2

Function: Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38

pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2.

Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in

neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at

'Ser-28'

Gene Wiki entry for ZAK

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ZAK gene promoter:
         SRF   Cart-1   C/EBPalpha   C/EBPbeta   FOXJ2 (long isoform)   NF-AT1   NF-AT3   NF-AT4   NF-AT2   Cdc5
         Other transcription factors

Search SABiosciences Chromatin IP Primers for ZAK

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q24.2 Ensembl cytogenetic band: 2q31.1

ZAK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZAK gene location

GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure

GeneLoc location for GC02P165819: view genomic region (about GC identifiers)

Start:	165,819,879 bp from pter
End:	166,012,160 bp from pter
Size:	192,282 bases
Orientation:	plus strand

1 alternative location:

Chr2+ 173,940,442-174,132,738

RefSeq DNA sequence:

NC_000002.11 NT_005403.17

(According to ¹UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: MLTK_HUMAN, Q9NYL2 (See protein sequence)

Recommended Name: Mitogen-activated protein kinase kinase kinase MLT

Size: 800 amino acids; 91155 Da

Cofactor: Magnesium

Subunit: Homodimer. Interacts with PKN1 and ZNF33A

Subcellular location: Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation

Sequence caution: Sequence=BAD92211.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

Secondary accessions: B3KPG2 Q53SX1 Q580W8 Q59GY5 Q86YW8 Q9HCC4 Q9HCC5 Q9HDD2 Q9NYE9

Alternative splicing: 3 isoforms: Q9NYL2-1 Q9NYL2-2 Q9NYL2-3

Explore the universe of human proteins at neXtProt for ZAK: NX_Q9NYL2

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins (2 alternative transcripts):

NP_057737.2 NP_598407.1

ENSEMBL proteins:

ENSP00000387259 ENSP00000364361 ENSP00000399787 ENSP00000340257 ENSP00000411923

Human Recombinant Proteins

	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: ZAK OriGene Protein Over-expression Lysate: ZAK
	GenScript Custom Purified and Recombinant Proteins Services for ZAK
	Novus Biologicals Proteins for ZAK
	Novus Biologicals Lysate for ZAK
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins

2 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005737	cytoplasm	ISS	--

About this table

ZAK for ontologies About GeneDecksing

Antibodies for ZAK:

	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for ZAK
	Browse R&D Systems for Antibodies
	Browse OriGene Antibodies
	GenScript Custom Superior Antibodies Services for ZAK
	Novus Biologicals Antibodies for ZAK
	Epitomics antibodies for ZAK

Assays for ZAK:

	Browse Kits and Assays available from Millipore
	Browse ELISAs at Sigma-Aldrich
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for ZAK
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for ZAK

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ZAK for domains About GeneDecksing

5/8 InterPro domains/families (see all 8):

IPR013761 SAM_type

IPR001660 SAM

IPR010993 Sterile_alpha_motif_homology

IPR021129 SAM_type1

IPR001245 Ser-Thr/Tyr-Pkinase

Graphical View of Domain Structure for InterPro Entry Q9NYL2

ProtoNet protein and cluster: Q9NYL2

2 Blocks protein families:

IPB001660 Sterile alpha motif SAM

IPB011510 Sterile alpha motif homology 2

UniProtKB/Swiss-Prot: MLTK_HUMAN, Q9NYL2

Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase

subfamily

Similarity: Contains 1 protein kinase domain

Similarity: Contains 1 SAM (sterile alpha motif) domain

(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI ,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: MLTK_HUMAN, Q9NYL2

Function: Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38

pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2.

Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in

neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at

'Ser-28'

Catalytic activity: ATP + a protein = ADP + a phosphoprotein

Enzyme regulation: Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165

Enzyme Number (IUBMB): EC 2.7.11.25

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Sigma-Aldrich Nano Scale siRNA for ZAK Sigma-Aldrich siRNA Panels and esiRNA and siRNA for ZAK Sigma-Aldrich shRNA Panels and shRNA for ZAK
		OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): ZAK OriGene shRNA RFP (see all 2): ZAK OriGene basic RS shRNA (see all 2): ZAK OriGene siRNA (see all 2): ZAK
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of ZAK

miRNA:		Sigma-Aldrich microRNA Mimics for ZAK Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of ZAK
		8/32 SABiosciences Assays for microRNAs that regulate ZAK (see all 32):
		hsa-miR-607 hsa-miR-15a hsa-miR-301a hsa-miR-342-3p hsa-miR-424 hsa-miR-130b hsa-miR-15b hsa-miR-301b

Gene
Editing:

Browse CompoZr Knockout ZFN at Sigma-Aldrich

Clones:		Browse Clones for the Expression of Recombinant Proteins Available from Millipore
		Browse iPSC Reprogramming Factors at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene untagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene 3'-UTR clone (see all 2): ZAK Browse MicroRNA Expression Plasmids
		GenScript Custom cDNA clone Services for ZAK
		Browse Sino Biological Human cDNA Clones

Cell
Lines:

GenScript Custom overexpressing Cell Line Services for ZAK

5/9 Gene Ontology (GO) molecular function terms (GO ID links to tree view) (see all 9):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	IEA	--
GO:0000287	magnesium ion binding	IDA	11836244
GO:0004672	protein kinase activity	--	--
GO:0004674	protein serine/threonine kinase activity	IDA	11836244
GO:0004709	MAP kinase kinase kinase activity	IDA	11836244

About this table

ZAK for ontologies About GeneDecksing

(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

ZAK for pathways About GeneDecksing

1 Millipore Pathway for ZAK

G-protein signaling Regulation of p38 and JNK signaling mediated by G-proteins

1 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathway for ZAK

SAPK/JNK Signaling

2 Kegg Pathways (Kegg details for ZAK):

	hsa04010 MAPK signaling pathway
	hsa04530 Tight junction

SABiosciences Pathway-Focused PCR Arrays including ZAK: PAHS-029A PAHS-3012A

Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for ZAK

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for ZAK

5/7 Interacting proteins for ZAK (ENSP00000364361³ Q9NYL2^{1, 2}) via UniProtKB, MINT, and/or STRING (see all 7)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ZAK	Q9NYL2¹	EBI-602273,EBI-602273
SFN	P31947²	MINT-3975752
ZNF567	Q8N184²	MINT-67507
PKN1	ENSP00000343325³	STRING: ENSP00000343325
TJP1	ENSP00000281537³	STRING: ENSP00000281537

About this table

5/15 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 15):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000075	cell cycle checkpoint	IDA	15342622
GO:0000077	DNA damage checkpoint	IMP	11836244
GO:0000186	activation of MAPKK activity	TAS	11836244
GO:0006468	protein phosphorylation	IDA	11836244
GO:0006950	response to stress	NAS	11836244

About this table

ZAK for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

ZAK for compounds About GeneDecksing

Browse Small Molecules at Sigma-Aldrich

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for ZAK
3 Novoseek chemical compound relationships for ZAK gene

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
leucine	34.3	4	11549352 (1)
threonine	33.3	1	11549352 (1)
serine	18.8	1	11549352 (1)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build ; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Sigma-Aldrich Nano Scale siRNA for ZAK Sigma-Aldrich siRNA Panels and esiRNA and siRNA for ZAK Sigma-Aldrich shRNA Panels and shRNA for ZAK
		OriGene 29mer shRNA kits in GFP-retroviral vector (see all 2): ZAK OriGene shRNA RFP (see all 2): ZAK OriGene basic RS shRNA (see all 2): ZAK OriGene siRNA (see all 2): ZAK
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of ZAK

miRNA:		Sigma-Aldrich microRNA Mimics for ZAK Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of ZAK
		8/32 SABiosciences Assays for microRNAs that regulate ZAK (see all 32):
		hsa-miR-607 hsa-miR-15a hsa-miR-301a hsa-miR-342-3p hsa-miR-424 hsa-miR-130b hsa-miR-15b hsa-miR-301b

Clones:		Browse FLAG tag genes at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene untagged cDNA clones in CMV expression vector (see all 2): ZAK OriGene 3'-UTR Clone (see all 2): ZAK Browse OriGene MicroRNA Expression Plasmids
		GenScript Custom cDNA clone Services for ZAK

Primers:		OriGene genome-wide validated SYBR primer pairs: ZAK Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for ZAK: PPH05541A

REFSEQ mRNAs for ZAK gene (2 alternative transcripts):

NM_016653.2 NM_133646.2

Additional cDNA sequence:

AB030034.1 AB041883.1 AB049733.1 AB049734.1 AB208974.1 AF238255.1 AF251441.1 AF325454.1

AF465843.1 AF480461.1 AF480462.1 AK056310.1 AK095825.1 AK096877.1 AK291825.1 AK298634.1

AL833180.1 BC001401.2 CR598198.1 CR606268.1

10 DOTS entries:

DT.423171 DT.452501 DT.87016635 DT.100783996 DT.91811205 DT.100678385 DT.120976575 DT.100028728

DT.95167694 DT.99932386

24/380 AceView cDNA sequences (see all 380):

AI276448 AI470677 AB049734 AW516988 BM755642 BF508076 AI346235 AA232772

BU608932 BC001401 CD676494 BQ775913 AI066646 AI452885 AI858232 AI468464

AA307684 CA397545 AI023521 R91202 AL701472 AI096766 CN479035 AW502467

highest scoring ESTs for ZAK:

AI799498 N95341 AA100075 AA236466 AA258354 AA307684 AA402496 AA402558 AA505991 AA626441

Unigene Cluster for ZAK:

Sterile alpha motif and leucine zipper containing kinase AZK

Hs.444451 [show with all ESTs]

Unigene Representative Sequence:

GeneLoc Exon Structure

8 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000409176(uc002uia.1) ENST00000375213(uc002uhz.2) ENST00000476618(uc002uhx.2)

ENST00000431503 ENST00000480606 ENST00000338983(uc002uhy.2 uc010zei.1)

ENST00000422149 ENST00000468408

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build ), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

ZAK expression in normal and diseased human tissues

¹ / ² / ³

20 probe-sets matching ZAK gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
85944_at²^{, 3}	U95-E	1	1.00	1.00	0.82	0.76	AA307684	0.80	1.00	0.91	1
76950_at²^{, 3}	U95-E	1	0.94	1.00	0.85	0.66	AI129413	0.60	1.00	0.82	1
61778_at²^{, 3}	U95-C	1	0.94	1.00	-0.18	0.87	N95341	1.00	1.00	1.00	1
65702_at²^{, 3}	U95-C	1	0.94	1.00	0.96	1.03	AA425744	0.60	1.00	0.82	1
60464_at²^{, 3}	U95-C	1	0.88	1.00	0.02	1.02	AI799498	1.00	1.00	1.00	1
48980_at²^{, 3}	U95-C	1	0.69	1.00	0.93	1.24	AI375684	0.80	1.00	0.91	1
54713_at²^{, 3}	U95-B	1	0.56	1.00	0.97	1.60	AI129320	0.80	1.00	0.91	1
222757_s_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AB030034	0.80	1.00	0.91	1
238613_at²^{, 3}	U133-B	1	0.91	1.00	--	--	AI475164	0.40	1.00	0.76	1
223519_at²^{, 3}	U133-B	1	0.91	1.00	--	--	AW069181	0.40	1.00	0.76	1
218833_at²^{, 3}	U133-A	1	0.73	1.00	--	--	NM_016653	0.60	1.00	0.82	1
225665_at²^{, 3}	U133-B	1	0.64	1.00	--	--	AI129320	0.80	1.00	0.91	1
225662_at²^{, 3}	U133-B	1	0.64	1.00	--	--	H28667	0.60	1.00	0.82	1
222757_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
238613_at²	U133Plus2	1	0.91	1.00	--	--	--	--	--	--	--
223519_at²	U133Plus2	1	0.91	1.00	--	--	--	--	--	--	--
218833_at²	U133Plus2	1	0.73	1.00	--	--	--	--	--	--	--
225665_at²	U133Plus2	1	0.64	1.00	--	--	--	--	--	--	--
225662_at²	U133Plus2	1	0.64	1.00	--	--	--	--	--	--	--
1555259_at²	U133Plus2	1	0.55	1.00	--	--	--	--	--	--	--

About this table

ZAK for expression About GeneDecksing

Data from Genenote (Publications) and GNF BioGPS
About these images

About these images

CGAP SAGE TAG: CCAGTAACCC

SOURCE GeneReport for Unigene cluster: Hs.444451

UniProtKB/Swiss-Prot: MLTK_HUMAN, Q9NYL2

Tissue specificity: Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for

liver, in which isoform 1 is more highly expressed

Primers:		OriGene genome-wide validated SYBR primer pairs: ZAK Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for ZAK: PPH05541A

SABiosciences Expression via Pathway-Focused PCR Arrays including ZAK: PAHS-029A PAHS-3012A

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI , with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for ZAK gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC478802¹	--	similar to mixed lineage kinase-related kinase MRK-beta more	91.12(n) 92.12(a)	478802 XM_535966.2 XP_535966.2
mouse (Mus musculus)	B230120H23Rik¹	--	RIKEN cDNA B230120H23 gene	87.65(n) 92.24(a)	65964 NM_023057.2 NP_075544.1
chicken (Gallus gallus)	ZAK¹	--	sterile alpha motif and leucine zipper containing kinase more	75.02(n) 76.58(a)	424149 XM_421996.2 XP_421996.2
zebrafish (Danio rerio)	LOC559247¹	--	similar to Mitogen-activated protein kinase kinase more	63.24(n) 62.94(a)	559247 XM_682568.2 XP_687660.2
tropical clawed frog (Silurana tropicalis)	AL898835.2²	--	--	81.39(n)	AL898835.2

About this table Species with no ortholog for ZAK

ENSEMBL Gene Tree for ZAK (if available)

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for ZAK gene

TNNI3K² KSR1² MAP3K12² MAP3K13² KSR2² ILK²

ZAK for paralogs About GeneDecksing

(SNPs according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

SNP ID	Valid	Chr 2 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample
Genomic Data				Transcription Related Data				Allele Frequencies
			--	--	--		--	--
rs8446^1,2	C,F,	174090542(+)	`AAAAAC/TGGTTT`	2	--	int¹ ut3¹		19	MN NS EA NA WA	1332
rs11249^1,2	C,F,O,A,H,	174086672(+)	`GACACG/ATAGTA`	2	--	ut3¹ int¹ ese³		19	MN NS EA NA WA	1443
rs722864^1,2	C,F,A,	173983204(-)	`CCCCCA/C/TCAAAA`	2	--	int¹		13	EA NA NS WA	2230
rs734993^1,2	C,F,A,	174025999(+)	`AGTGAA/CCTAGT`	2	--	int¹		14	MN NS EA NA WA	2460
rs744075^1,2	--	174026854(-)	`TATTGT/GGGTCA`	2	--	int¹		4	EA NA MN	238
rs746179^1,2	C,F,	174026324(+)	`ATTTTA/GTAAGA`	2	--	int¹		14	MN NS EA NA WA	2440
rs820006^1,2	C,H,	174129772(+)	`CACAAT/CAACAA`	1	--	int¹		14	EA NA NS WA	520
rs820007^1,2	C,F,O,A,	174123273(+)	`ATAGGA/CATCTA`	1	--	int¹		18	NA EA NS WA	1388
rs820008^1,2	C,F,A,	174123399(+)	`TCTCAG/AATTCT`	1	--	int¹		8	NS NA EA WA	338
rs820009^1,2	C,F,H,	174124316(+)	`GGGGCT/GTGTAG`	1	--	int¹		20	NA EA MN NS WA	1050

About this table

HapMap Linkage Disequilibrium images for ZAK (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ZAK: --

QIAGEN SeqTarget long-range PCR primers for resequencing ZAK

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

ZAK for disorders About GeneDecksing

OMIM: 609479

2 Novoseek disease relationships for ZAK gene

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
hypertrophy	47	3	15485649 (1), 19184368 (1), 15465036 (1)
cardiac hypertrophy	43.9	2	11549352 (1), 15465036 (1)

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Human Genome Epidemiology (HuGE) Navigator: ZAK (26 documents)

Export disorders and mutations for ZAK gene to outside databases

(Possibly Related Articles in Doctor's Guide)
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Improved Imaging for Identifying Breast Cancer in Overweight Women

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶UniProtKB/TrEMBL, and/or ⁷Novoseek)
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10/39 PubMed articles for ZAK gene, integrated from 7 sources (see all 39):
(articles sorted by number of sources associating them with ZAK)

MRK, a mixed lineage kinase-related molecule that plays a role in gamma-radiation-induced cell cycle arrest. (PubMed id 11836244)^{1, 2, 7} Gross E.A.... Ruggieri R. (2002)
Tissue distribution and functional expression of a cDNA encoding a novel mixed lineage kinase. (PubMed id 11549352)^{1, 2, 7} Bloem L.J.... Wang X. (2001)
Identification and characterization of a novel MAP kinase kinase kinase, MLTK. (PubMed id 11042189)^{1, 2, 7} Gotoh I.... Nishida E. (2001)
Cloning and expression of ZAK, a mixed lineage kinase-like protein containing a leucine-zipper and a sterile-alpha motif. (PubMed id 10924358)^{1, 2, 7} Liu T.-C.... Yang J.-J. (2000)
Regulation of a mitogen-activated protein kinase kinase kinase, MLTK by PKN. (PubMed id 12761180)^{1, 2, 7} Takahashi M.... Ono Y. (2003)
Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. (PubMed id 17192257)^{1, 2} Wissing J.... Daub H. (2007)
A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)^{1, 2} Beausoleil S.A.... Gygi S.P. (2006)
Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha. (PubMed id 15684425)^{1, 2} Choi H.S.... Dong Z. (2005)
Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)^{1, 2} Hillier L.W....Wilson R.K. (2005)
The stress kinase MRK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. (PubMed id 15342622)^{1, 2} Tosti E.... Ruggieri R. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 51776

AceView: ZAK

Ensembl:ENSG00000091436

euGenes: HUgn51776

Kegg: 51776

(According to HUGE)
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(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Intellectual Property for ZAK gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from XenneX, Inc.)

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Patent Information for ZAK gene:
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protein-coding GIFtS: 51

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ZAK (Gene Symbol)
MRK
Leucine zipper- and sterile alpha motif-containing kinase
Human cervical cancer suppressor gene 4 protein
Sterile alpha motif- and leucine zipper-containing kinase AZK
MLK-like mitogen-activated protein triple kinase
HCCS-4
MLK-related kinase
MLTK
AZK
MLT
sterile alpha motif and leucine zipper containing kinase AZK
mixed lineage kinase-related kinase MRK-beta
OTTHUMP00000205097
MLK7
mlklak
pk
cervical cancer suppressor gene 4 protein
mitogen-activated protein kinase kinase kinase MLT
mixed lineage kinase 7
protein kinase
mixed lineage kinase with a leucine zipper and a sterile alpha motif
EC 2.7.11.25
Mixed lineage kinase-related kinase

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ZAK Gene

protein-coding GIFtS: 51

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